circBICD2 targets miR‐149‐5p/IGF2BP1 axis to regulate oral squamous cell carcinoma progression

Abstract

Background Circular RNAs (circRNAs) are related to oral squamous cell carcinoma (OSCC) progression. circRNA bicaudal D cargo adaptor 2 (circBICD2) has been reported to be abnormally expressed in OSCC. However, the function and mechanism of this circRNA in OSCC progression remain largely unknown. Methods circBICD2, microRNA‐149‐5p (miR‐149‐5p), and insulin‐like growth factor 2 mRNA‐binding protein 1 (IGF2BP1) abundances were examined via quantitative reverse transcription polymerase chain reaction or Western blot. The function of circBICD2 was investigated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), colony formation, flow cytometry, wound healing, transwell, specific kits, Western blot, and xenograft analyses. Dual‐luciferase reporter analysis and RNA immunoprecipitation were carried out to analyze the binding interaction. Results circBICD2 expression was enhanced in OSCC tissues and cells. circBICD2 silence suppressed OSCC cell proliferation, migration, invasion, and glutaminolysis and facilitated apoptosis. miR‐149‐5p was targeted via circBICD2 and decreased in OSCC tissues and cells. miR‐149‐5p knockdown attenuated silence of circBICD2 on the influence of OSCC cell proliferation, apoptosis, migration, invasion, and glutaminolysis. IGF2BP1 was targeted via miR‐149‐5p, and circBICD2 could regulate IGF2BP1 via miR‐149‐5p. IGF2BP1 interference constrained OSCC cell proliferation, migration, invasion, and glutaminolysis and promoted apoptosis. circBICD2 silence reduced OSCC cell growth in xenograft model. Conclusion circBICD2 knockdown repressed OSCC cell proliferation, migration, invasion, and glutaminolysis and increased apoptosis via modulating miR‐149‐5p/IGF2BP1 axis, which might act as a potential target for OSCC treatment.

CircRNA HIPK3 promotes the progression of oral squamous cell carcinoma through upregulation of the NUPR1/PI3K/AKT pathway by sponging miR-637

“…Several studies confirmed that circRNAs were ceRNAs of miRNA. For instance, circBICD2 enhanced IGF2BP1 as ceRNA via miR-149-5p, thus accelerating the proliferation and migration of OSCC cells (28). In addition, overexpressed circPHIP downregulated miR-142-5p and upregulated PHIP and ACTN4, thus aggravating OSCC (29).…”

Circular RNA mitochondrial translation optimization 1 homologue (CircMTO1) induced by zinc finger protein 460 (ZNF460) promotes oral squamous cell carcinoma progression through the microRNA miR-320a / alpha thalassemia/mental retardation, X-linked (ATRX) axis

“…Hsa_circRNA_100533 inhibits OSCC development through regulating GNAS by targeting hsa_miR_933 [ 29 ]. CircBICD2 regulates OSCC progression by targeting miR-149-5p/IGF2BP1 [ 30 ]. Based on the crucial role of the circRNA/miRNA/mRNA network in OSCC development, we performed bioinformatics analysis and a serial experiment to demonstrate the underlying mechanisms of circMTO1.…”

MiR-149-5p: An Important miRNA Regulated by Competing Endogenous RNAs in Diverse Human Cancers

“…Functional analysis showed that miR-149-5p could enhance the chemosensitivity of OSCC cells to cisplatin by targeting TGF b2, inhibit cell proliferation, migration and invasion, and promote apoptosis (75). Notably, Qiu et al confirmed that CircBICD2 knockdown could inhibit the proliferation, migration, invasion, glutamine degradation and increase apoptosis of OSCC cells by regulating the miR-149-5p/IGF2BP1 axis (70). Similarly, down-regulation of lncRNA DLEU1 could inhibit the occurrence of OSCC by regulating the miR-149-5p/CDK6 axis (76).…”

“…In addition to GC, HCC and CRC, miR-149-5p has also been studied in other digestive system cancers, including oral cancer, esophageal cancer and ductal adenocarcinoma of the pancreas (70)(71)(72)(73)(74)(75)(76).…”