CircRNA microarray profiling identifies a novel circulating biomarker for detection of gastric cancer

Tang, Weiwei; Fu, Kai; Sun, Handong; Ding, Rong; Wang, Hanjin; Cao, Hongxin

doi:10.1186/s12943-018-0888-8

Cited by 215 publications

(165 citation statements)

References 6 publications

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“…Therefore, circulating tumor circRNAs (ct circRNAs) can be applied as potential signatures for the diagnosis of cancer. Tang et al (13) had demonstrated that plasma circ-KIAA1244 might be a potential signature in patients with GC. As far as we know, however, there are few studies on circRNAs in the plasma of patients with GC.…”

Section: Introductionmentioning

confidence: 99%

A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

et al. 2020

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Gastric cancer (GC) is the TOP3 leading cause of human mortality in malignant tumors. Notwithstanding, the association between GC and circRNAs is not clear. The purpose of this research was to determine the association between GC progression and circRNAs. The data of circRNAs was obtained from the Gene Expression Omnibus (GEO) database to identify gene, which differentially expressed circRNAs in GC tissues and paired normal tissues. The expression of circRNAs in cancer tissues and normal tissues were tested, and the target circRNA was verified before and after surgery in the plasma. A circRNA-micro(mi)RNA-mRNA competing endogenous RNAs (ceRNAs) network was established, and GO and KEGG analysis are performed. Five candidate circRNAs were identified through bioinformatics analysis. Hsa_circ_0021087 and hsa_circ_0005051 were both downregulated in GC tissues, cells and plasma by RTq-PCR. Additionally, there was a significant difference in the expression of plasma hsa_circ_0021087 in patients with GC at the preoperative and postoperative stages (P < 0.001). Hsa_circ_0021087 also promoted the proliferation of GC cells in vitro. Next, the circRNA-miRNA-mRNA network of hsa_circ_0021087 was predicted, which may be associated with the development of GC by bioinformatics analysis. In summary, the aforementioned dual-circular RNAs may have important implications on the potential, novel and non-invasive diagnostic method for patients with GC.

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Section: Introductionmentioning

confidence: 99%

A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

et al. 2020

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“…9 In gastric cancer, circ-KIAA1244 is correlated with cancer progression and patients' outcome. 10 In non-small cell lung cancer, hsa_circ_0033155 acts as a tumour suppressor to regulate cell proliferation, colony formation and migration. 11 However, there are only a few reports exploring circRNAs in ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

circEPSTI1 regulates ovarian cancer progression via decoying miR‐942

Xie

Wang

et al. 2019

J Cellular Molecular Medi

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Increasing studies show that circular RNAs (circRNAs) play vital roles in tumour progression. But, how circRNAs function in ovarian cancer is mostly unclear. Here, we detected the expression of circEPSTI1 in ovarian cancer and explored the function of circEPSTI1 in ovarian cancer via a series of experiments. Then, we performed luciferase assay and RNA immunoprecipitation (RIP) assay to explore the competing endogenous RNA (ceRNA) function of circEPSTI1 in ovarian cancer. qRT‐PCR verified that circEPSTI1 was overexpressed in ovarian cancer. Inhibition of circEPSTI1 suppressed ovarian cancer cell proliferation, invasion but promoted cell apoptosis. Luciferase assays and RIP assay showed that circEPSTI1 and EPSTI1 (epithelial stromal interaction 1) could directly bind to miR‐942. And circEPSTI1 could regulate EPSTI1 expression via sponging miR‐942. In summary, circEPSTI1 regulated EPSTI1 expression and ovarian cancer progression by sponging miR‐942. circEPSTI1 could be used as a biomarker and therapeutic target in ovarian cancer.

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“…Increasing evidence indicated that circRNAs act as potential biomarkers in patients with GC. Upregulation of circ-DON-SON, circAKT3, and circDLST [8,11,13] or downregulation of KIAA1244, circPSMC3, and circFAT1(e2) [14][15][16] is regarded as independent prognostic factors of poor prognosis in patients with GC. Herein, we identified a novel circDUSP16, and it was resistant to RNase R and localized in the cytoplasm of GC cells.…”

Section: Discussionmentioning

confidence: 99%

“…CircAKT3 and circDLST act as the sponges of miR-198/-502-5p to favor the tumorigenesis and cisplatin resistance in GC cells [11,13]. In addition, circ-KIAA1244 [14], circPSMC3 [15], and circFAT1(e2) [16] are downregulated in GC tissues and plasmas, and their decreased expression is related to tumor invasiveness and poor survival in GC patients [14][15][16]. These circRNAs may provide potential biomarkers for the treatment of GC.…”

Section: Introductionmentioning

confidence: 99%

CircDUSP16 promotes the tumorigenesis and invasion of gastric cancer by sponging miR-145-5p

et al. 2019

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Background Circular RNAs (circRNAs) as a novel subgroup of non-coding RNAs act a critical role in the pathogenesis of gastric cancer (GC). However, the underlying mechanisms by which hsa_circ_0003855 (circDUSP16) contributes to GC are still undocumented. Materials The differentially expressed circRNAs were identified by GEO database. The association of circDUSP16 or miR-145-5p expression with clinicopathological features and prognosis in GC patients was analyzed by FISH and TCGA-seq data set. Loss-and gain-of-function experiments as well as a xenograft tumor model were performed to assess the role of circDUSP16 in GC cells. circDUSP16-specific binding with miR-145-5p was confirmed by bioinformatic analysis, luciferase reporter, and RNA immunoprecipitation assays. Results The expression levels of circDUSP16 were markedly increased in GC tissue samples and acted as an independent prognostic factor of poor survival in patients with GC. Knockdown of circDUSP16 repressed the cell viability, colony formation, and invasive potential in vitro and in vivo, but ectopic expression of circDUSP16 reversed these effects. Moreover, circDUSP16 possessed a co-localization with miR-145-5p in the cytoplasm, and acted as a sponge of miR-145-5p, which attenuated circDUSP16-induced tumor-promoting effects and IVNS1ABP expression in GC cells. MiR-145-5p had a negative correlation with circDUSP16 expression and its low expression was associated with poor survival in GC patients. Conclusions CircDUSP16 facilitates the tumorigenesis and invasion of GC cells by sponging miR-145-5p, and may provide a novel therapeutic target for GC.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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CircRNA microarray profiling identifies a novel circulating biomarker for detection of gastric cancer

Cited by 215 publications

References 6 publications

A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

circEPSTI1 regulates ovarian cancer progression via decoying miR‐942

CircDUSP16 promotes the tumorigenesis and invasion of gastric cancer by sponging miR-145-5p

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