Circular linkage map of Rhizobium meliloti chromosome

Kondorosi, Ádám; Kiss, G. B.; Forrai, T.; Vincze, Éva; Bánfalvi, Zsófia

doi:10.1038/268525a0

Cited by 146 publications

(74 citation statements)

References 14 publications

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“…aeruginosa, Ps. putida (Haas & Holloway, 1976), R. leguminosarum, E. coli (Beringer & Hopwood, 1976) and R. meliloti (Kondorosi et al, 1977), but fails to do so in P. mirabilis (unpublished observations). Specificity is thought (Jacob et al, 1976(Jacob et al, , 1977 to depend on the presence of homologous insertion sequences (ISs) on chromosome and plasmid, respectively, in much the same manner as the F factor of E. coli mobilizes that chromosome (Hu et al, 1975).…”

Section: Discussionmentioning

confidence: 99%

“…With other P-1 plasmids the requirements for donor ability are less strict but still very specific. Thus RP4 does not mobilize the Pseudomonas aeruginosa chromosome (Stanisich & Holloway, 197 1 ; Haas & Holloway, I976), has only marginal activity in Rhizobium leguminosarum (Beringer & Hopwood, 1976) but promotes chromosome transfer in R. meliloti (Meade & Signer, 1977) and Acinetobacter calcoaceticus (Towner & Vivian, 1976a, b, 1977. Another member of the P-1 group, R68.45, promotes chromosome transfer in strains of Ps.…”

Section: Discussionmentioning

confidence: 99%

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Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Coetzee

1978

Journal of General Microbiology

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The P-1 incompatibility group plasmid R772 can mobilize the chromosome of Proteus mirabilis strain ~~5 0 0 6 .The decreasing gradient of recombinant recovery frequencies found for markers which were increasingly distal to 0 min with plasmid D donors was not found with R772. Instead, it produced recombinants for all markers at frequencies of about 5 x per donor. This is about 10-fold lower than the plasmid transfer frequency. Recombinants were stable and recombination was only detected over short segments of the chromosome which corresponded to about 10 min on the D plasmid map of the chromosome. All recombinants had inherited R772 and expressed all properties of the plasmid. Attempts to isolate variant plasmids with increased frequencies of recombinant formation were unsuccessful.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Coetzee

1978

Journal of General Microbiology

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“…An advantage of the relatively short linkage maps of R. leguminosarum and R. meliloti (Meade & Signer, 1977;Kondorosi et al, 1977) is that only a few crosses need to be done to arrive at an approximate map location for any new mutations.…”

Section: Discussionmentioning

confidence: 99%

Linkage Mapping in Rhizobium leguminosarum by means of R Plasmid-mediated Recombination

Beringer¹,

Hoggan²,

Johnston³

1978

Journal of General Microbiology

115

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“…Single R plasmids of the multiple chromosomal association type, on the other hand, have been used in plate-mating experiments to derive linkage data which indirectly established circularity of chromosome linkage groups in Rhizobium meliloti (Meade & Signer, 1977;Kondorosi et al, 1977) and Rhizobium Ieguminosarum (Beringer et a]., 1978).…”

Section: Discussionmentioning

confidence: 99%

Genetic Circularity of the Proteus mirabilis Linkage Map

Coetzee

1979

Journal of General Microbiology

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171The T incompatibility group plasmid R394 can mobilize the chromosome of Pruteus mirabilis strain ~~5 0 0 6 .It transferred relatively large segments, corresponding to at least 20 inin on the D plasmid chromosomal map of the organism. The frequency of recombination for a large number of selected markers was nearly constant at 5 x lo-* per donor cell and it is concluded that mobilization takes place from a number of chromosomal sites. All recombinants were R f and displayed all properties of the plasmid. By analysing crosses for co-inheritance frequencies of unselected markers, a number of chromosomal loci were assembled in linear array. Linkage between markers at the ends of this linkage group was established to markers at the respective termini of the existing D plasmid linkage group. This established a composite circular linkage map of genes of the P. mirabilis strain ~~5 0 0 6 chromosome. I N T R O D U C T I O NMobilization of the chromosome of P. mirabilis strain ~~5 0 0 6 by the recombinant plasmid P-lacRldrd19 has been described (Coetzee, 1975). Polarized transfer occurred from an origin near his-2 and involved a gradient of eight markers. As a result of a modification in the conjugation procedure (Coetzee, 1978a) the linkage map was extended by a further eight chromosomal markers. This plasmid is now named plasmid D (Coetzee, 1978a).R plasmid R772 can also mobilize the ~~5 0 0 6 chromosome (Coetzee, 1978b). R772-mediated transfer occurred from a number of chromosomal sites and, possibly because predominantly short segments of chromosome were mobilized, co-inheritance of the terminal markers of the D plasmid-derived linkage group could not be demonstrated.A number of other R plasmids may mobilize the ~~5 0 0 6 chromosome (J. N. Coetzee and R. W. Hedges, unpublished observations) and the use of one of these, R394 (Coetzee et d., 1972), to establish indirectly the genetic circularity of the ~~5 0 0 6 chromosomal linkage group is described here. M E T H O D SBacteria and plasmid. These are listed in Table 1. Media. These were as described previously (Coetzee, 1978 b). Antibacterial drugs. Nalidixic acid, ampicillin, kanamycin, tetracycline (each 50 pg ml-I) or rifampicin (200 ,ug ml-I) were added to media when necessary.Plate matings. These were done by the method of Coetzee (1978a). Briefly, mating mixtures were constituted on non-selective MM (i.e. supplemented with growth requirements of recipient and donor) and incubated overnight before harvesting in saline. Control experiments lacked either donor or recipient. After washing, suitable dilutions were plated on selective MM and incubated for 48 h.Consfruction of P. mirabilis UP strains containing plasmid R394. The liquid mating technique of Coetzee et al. (1973) was used. Donor ~53(R394) was counterselected with tetracycline and transfer of the plasmid was selected with kanamycin or ampicillin. Transfer of the markers of plasmid R394 to various UP strains occurred at frequencies of about 1 x per donor cell. All donor strains were maintained on nutrient ...

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Circular linkage map of Rhizobium meliloti chromosome

Cited by 146 publications

References 14 publications

Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Mobilization of the Proteus mirabilis Chromosome by R Plasmid R772

Linkage Mapping in Rhizobium leguminosarum by means of R Plasmid-mediated Recombination

Genetic Circularity of the Proteus mirabilis Linkage Map

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