Circular RNA Atp9b, a competing endogenous RNA, regulates the progression of osteoarthritis by targeting miR-138-5p

Zhou, Zhengming; Du, Di; Huang, Gaoxiang; Chen, Aimin; Zhu, Lei

doi:10.1016/j.gene.2017.12.064

Cited by 89 publications

(75 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our result revealed that Circ_0136474 was highly expressed in OA cartilage tissues compared with normal cartilage tissues and played its role in the cytoplasm. In addition, the overexpression of Circ_0136474 suppressed cell proliferation yet enhanced cell apoptosis by regulating inflammatory mediators and collagen type II in OA, which was similar to the stimulative roles of Circ_Atp9b in OA progression 6. miRNAs controlled a large set of biological processes through direct interaction with their target mRNAs.…”

mentioning

confidence: 65%

“…Circular RNAs (circRNAs) are endogenous RNAs featuring for covalent loop structures with neither 5′ to 3′ polarity nor a polyadenylated tail . It was reported that circRNAs regulate the gene expression in both transcriptional and post‐transcriptional process .…”

Section: Introductionmentioning

confidence: 99%

“…Circular RNAs (circRNAs) are endogenous RNAs featuring for covalent loop structures with neither 5′ to 3′ polarity nor a polyadenylated tail. 6 It was reported that circRNAs regulate the gene expression in both transcriptional and post-transcriptional process. 7 Recently, circRNAs, novel noncoding RNAs with varied biological functions and pathological implications, have been shown to be closely related with various diseases, including osteoarthritis, atherosclerotic vascular disease risk, neurological disorders, Parkinson's disease, Alzheimer's disease, schizophrenia and so on.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circ_0136474 and MMP‐13 suppressed cell proliferation by competitive binding to miR‐127‐5p in osteoarthritis

Yuan

Pei

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

Osteoarthritis (OA) is a prevalent degenerative joint disease whose pathogenesis remains unclear. The research aims to investigate the roles of Circ_0136474/miR‐127‐5p/MMP‐13 axis in OA. Differentially expressed circRNAs and miRNAs in OA cartilage tissue were screened out and visualized by R project based on RNA‐seq data and microarray data respectively. qRT‐PCR was carried out for detection of relative expression levels of Circ_0136474, miR‐127‐5p, MMP‐13 and other inflammatory factors and Western blot analysis was conducted to detect the protein expression level of MMP‐13. CCK‐8 assay and flow cytometry were conducted to determine cell proliferation and cell apoptotic ability respectively. RNA‐fluorescence in situ hybridization (RNA‐FISH) experiments were conducted to confirm the immune‐localization of the Circ_0136474 and MMP‐13 in human tissues. Targeted relationships were predicted by bioinformatic analysis and verified by dual‐luciferase reporter assay. Our findings revealed that the expression levels of both Circ_0136474 and MMP‐13 in OA cartilage tissue were significantly higher than that in normal cartilage tissue. Circ_0136474 could suppress cell proliferation by facilitating MMP‐13 expression and suppressing miR‐127‐5p expression in OA. Overexpression of miR‐127‐5p negatively regulated MMP‐13 expression to enhance cell proliferation. Our study demonstrated that Circ_0136474 and MMP‐13 suppressed cell proliferation, while enhanced cell apoptosis by competitive binding to miR‐127‐5p in OA, which may well provide us with a new therapeutic strategy for osteoarthritis.

show abstract

mentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Circ_0136474 and MMP‐13 suppressed cell proliferation by competitive binding to miR‐127‐5p in osteoarthritis

Yuan

Pei

et al. 2019

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Previous studies have implicated circular RNAs as sponges for miR-136 in association with OA 34 , and have found substantial elevations of circRNAs in the synovial fluid of OA patients 35 . This year, we have two papers by Zhou and colleagues further elucidating the role of circRNAs in OA; the first demonstrating increases in the expression of a variety of circRNAs following IL1b treatment of mouse articular chondrocytes 36 , the other being a specific study of circRNA_Atp9b, which functions as a sponge for miR-138-5p, also implicated in the osteoarthritic response of mouse chondrocytes to IL1b treatment 37 .…”

Section: Epigenetics: Noncoding Rnasmentioning

confidence: 99%

Osteoarthritis year in review 2018: genetics and epigenetics

Jeffries

2019

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

Objective: This review was designed to identify highlights of the osteoarthritis (OA) genetics and epigenetics literature published between April 2017 and January 2018. Design: A Pubmed literature search was conducted using the keywords 'osteoarthritis' and each of the following: 'genomic', 'genetic', 'epigenomic', 'epigenetic', 'histone', 'noncoding RNA', 'miRNA', 'lncRNA', 'DNA methylation', 'DNA hydroxymethylation', 'DNMT', and 'TET'. The dates of publication were restricted to 4/1/2017e1/15/2018. Results were compared to the same search terms limited to 4/1/2016e1/15/2017. Results: Virtually all search term combinations demonstrated a decrease in papers published this year compared to last, with epigenetic and miRNA/lncRNA research being stable. Despite this, numerous advances were made this year, including the second large genome-wide association study (GWAS) study of hand OA, a new twin study of hip and knee OA concordance, an extensive study of GDF5 evolution, analyses of the contribution of Dnmt3a to OA, a description of DNA methylation in a nonhuman primate model of OA, and an integrated, multi-omics analysis of DNA methylation, mRNA, and protein expression in human OA samples, among others. A variety of micro-and a few circular-RNA studies were also published, highlighting the importance of noncoding RNA in both the pathogenesis and potential treatment of OA. Conclusion: Although publications have decreased slightly in the last year, genetics and epigenetics continue to be a topic of substantial research in OA, and considerable progress continues to be made in the field.

show abstract

“…For example, hsa_circ_0005105 and circRNA.33186 were shown to promote chondrocyte apoptosis by activating the miR‐26a/NAMP axis and negatively regulating miR‐127‐5p (Wu, Zhang, et al, ; Zhou, Huang, et al, ). In addition, circRNA_Atp9b was found to promote chondrocyte apoptosis in a mouse model of OA by negatively regulating miR‐138‐5p (Zhou et al, ).…”

Section: Chondrocyte Injuriesmentioning

confidence: 99%

Noncoding RNAs: New regulatory code in chondrocyte apoptosis and autophagy

Jiang

Liu

et al. 2020

WIREs RNA

View full text Add to dashboard Cite

Osteoarthritis (OA) is a bone and joint disease characterized by progressive cartilage degradation. In the face of global trends of population aging, OA is expected to become the fourth most common disabling disease by 2020. Nevertheless, the detailed pathogenesis of OA has not yet been elucidated. Noncoding RNAs (ncRNAs), including long noncoding RNAs, microRNAs, and circular RNAs, do not encode proteins but have recently emerged as important regulators of apoptosis and autophagy of chondrocytes, thereby highlighting a potential role in chondrocyte injury leading to OA onset and progression. We here review recent findings on these regulatory roles of ncRNAs to provide new directions for research on the pathogenesis of OA and offer new therapeutic targets for prevention and treatment. This article is categorized under: RNA in Disease and Development > RNA in Disease RNA in Disease and Development > RNA in Development

show abstract

Circular RNA Atp9b, a competing endogenous RNA, regulates the progression of osteoarthritis by targeting miR-138-5p

Cited by 89 publications

References 29 publications

Circ_0136474 and MMP‐13 suppressed cell proliferation by competitive binding to miR‐127‐5p in osteoarthritis

Circ_0136474 and MMP‐13 suppressed cell proliferation by competitive binding to miR‐127‐5p in osteoarthritis

Osteoarthritis year in review 2018: genetics and epigenetics

Noncoding RNAs: New regulatory code in chondrocyte apoptosis and autophagy

Contact Info

Product

Resources

About