Circular RNA Circ_0000064 promotes the proliferation and fibrosis of mesangial cells via miR-143 in diabetic nephropathy

Ge, Xin; Xi, Liuqing; Wang, Qianqian; Li, Huihua; Xia, Lili; Cang, Zhen; Peng, Wenfang; Huang, Shan

doi:10.1016/j.gene.2020.144952

Cited by 41 publications

(25 citation statements)

References 42 publications

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“…Recently, increasing circRNAs are identified to exhibit crucial roles in developmental processes and dysregulation of circRNAs is correlated with many diseases [23, 24]. Some circRNAs have been found to be associated with DN, such as circ_0080425 [25], circ_0000064 [26], and circ_0000285 [27]. More importantly, circ_0000491 was reported to be significantly augmented in both DN mouse model and HG-simulated mouse mesangial cells, and circ_0000491 knockdown inhibited ECM accumulation and fibrosis in HG-induced mouse mesangial cells [15].…”

Section: Discussionmentioning

confidence: 99%

Circ_0000491 Promotes Apoptosis, Inflammation, Oxidative Stress, and Fibrosis in High Glucose-Induced Mesangial Cells by Regulating miR-455-3p/Hmgb1 Axis

Wang

Yang

et al. 2021

Nephron

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Background: Diabetic nephropathy (DN) is a severe microvascular complication of diabetes. Recently, many circular RNAs can exert crucial roles in DN progression. This study intended to explore the role and mechanism of circ_0000491 in DN. Methods: The DN mouse model was constructed by streptozotocin injection, and the DN cell model was established using high glucose (HG) treatment in mouse mesangial cells (SV40-MES13). The expression of circ_0000491 and microRNA-455-3p (miR-455-3p) was detected by quantitative real-time polymerase chain reaction. Cell apoptosis was evaluated by flow cytometry. The expression levels of high-mobility group box 1 (Hmgb1) protein, apoptosis-related proteins, and fibrosis-related proteins were examined by the Western blot assay. The release of inflammatory cytokines was assessed by enzyme-linked immunosorbent assay. The oxidative stress factors were analyzed by corresponding kits. The predicted interaction between miR-455-3p and circ_0000491 or Hmgb1 was verified by dual-luciferase reporter assay and RNA immunoprecipitation assay. Results: Circ_0000491 was overexpressed in the DN mouse model and HG-induced SV40-MES13 cells. Knockdown of circ_0000491 weakened HG-induced apoptosis, inflammation, oxidative stress, and fibrosis in SV40-MES13 cells. miR-455-3p was a direct target of circ_0000491, and miR-455-3p inhibition could reverse the role of circ_0000491 silencing in HG-induced SV40-MES13 cells. Moreover, Hmgb1 was a target gene of miR-455-3p, and miR-455-3p played a protective role against HG-induced cell injury by targeting Hmgb1. In addition, circ_0000491 regulated Hmgb1 expression by sponging miR-455-3p. Conclusion: Circ_0000491 knockdown inhibited HG-induced apoptosis, inflammation, oxidative stress, and fibrosis in SV40-MES13 cells by regulating miR-455-3p/Hmgb1 axis.

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Section: Discussionmentioning

confidence: 99%

Circ_0000491 Promotes Apoptosis, Inflammation, Oxidative Stress, and Fibrosis in High Glucose-Induced Mesangial Cells by Regulating miR-455-3p/Hmgb1 Axis

Wang

Yang

et al. 2021

Nephron

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“…Finally, even though their exact mechanistic role remains relatively elusive, sscRNAs have been associated with contribution to fibrosis [124]. In diabetic nephropathy, the knockdown of various sscRNAs, including circ_0123996 [125], circ_0000064 [126] and circ_WBSCR17 [127], in mesangial and tubular cells by siRNA or miRNA mimetics resulted in reduction of fibronectin, collagen, and TGF-β1. Mechanistic exploration of the role of these sscRNAs revealed that their pro-fibrotic actions rely on the sponging of endogenous regulator miRNAs that inhibit the production of fibrotic proteins.…”

Section: Mirna Lncrna and Sscrna Nucleotides As Anti-fibrotic Targetsmentioning

confidence: 99%

Therapeutic and diagnostic targeting of fibrosis in metabolic, proliferative and viral disorders

Sofias

Lorenzi

Peña

et al. 2021

Advanced Drug Delivery Reviews

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“…Interestingly, results of several studies in DKD indicated that different circRNAs could act as the same miRNA's sponge. For example, miR-143 was confirmed to be targeted by both circ_DLGAP4 and circ_0000064 in MCs, whose increased expression consistently promoted cell proliferation and fibrosis ( 71 , 101 ). As mentioned above, circ_WBSCR17 functioned through sponging miR-185-5p in TECs.…”

Section: Circrnas As Therapeutic Targets For Kidney Diseasesmentioning

confidence: 99%

Circular RNAs as Novel Diagnostic Biomarkers and Therapeutic Targets in Kidney Disease

Xie²,

Huang

et al. 2021

Front. Med.

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Circular RNAs (circRNAs) are a novel type of non-coding RNAs that have aroused growing attention in this decade. They are widely expressed in eukaryotes and generally have high stability owing to their special closed-loop structure. Many circRNAs are abundant, evolutionarily conserved, and exhibit cell-type-specific and tissue-specific expression patterns. Mounting evidence suggests that circRNAs have regulatory potency for gene expression by acting as microRNA sponges, interacting with proteins, regulating transcription, or directly undergoing translation. Dysregulated expression of circRNAs were found in many pathological conditions and contribute to the pathogenesis and progression of various disorders, including renal diseases. Recent studies have revealed that circRNAs may serve as novel reliable biomarkers for the diagnosis and prognosis prediction of multiple kidney diseases, such as renal cell carcinoma (RCC), acute kidney injury (AKI), diabetic kidney disease (DKD), and other glomerular diseases. Furthermore, circRNAs expressed by intrinsic kidney cells are shown to play a substantial role in kidney injury, mostly reported in DKD and RCC. Herein, we review the biogenesis and biological functions of circRNAs, and summarize their roles as promising biomarkers and therapeutic targets in common kidney diseases.

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Circular RNA Circ_0000064 promotes the proliferation and fibrosis of mesangial cells via miR-143 in diabetic nephropathy

Cited by 41 publications

References 42 publications

Circ_0000491 Promotes Apoptosis, Inflammation, Oxidative Stress, and Fibrosis in High Glucose-Induced Mesangial Cells by Regulating miR-455-3p/Hmgb1 Axis

Circ_0000491 Promotes Apoptosis, Inflammation, Oxidative Stress, and Fibrosis in High Glucose-Induced Mesangial Cells by Regulating miR-455-3p/Hmgb1 Axis

Therapeutic and diagnostic targeting of fibrosis in metabolic, proliferative and viral disorders

Circular RNAs as Novel Diagnostic Biomarkers and Therapeutic Targets in Kidney Disease

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