Circular RNAs in cancer: an emerging key player

Dong, Yeping; He, Dan; Zhang, Peng; Peng, Wei; Shi, Weisong; Wang, Jun; Li, Bin; Zhang, Chunfang; Duan, Chaojun

doi:10.1186/s13045-016-0370-2

Cited by 243 publications

(210 citation statements)

References 56 publications

(70 reference statements)

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“…Similar to lncRNA, circRNAs were mainly used as a miRNA sponge to compete with miRNAs, reversing the inhibitory effect of miRNA downstream genes, thus increasing the expression of target genes [31]. Memczak found that CDR1as had 63 conservative binding sites to miR-7 in nervous tissue.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8

Liu

Wang

Long

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs) play a significant role in the development and progression of various human cancers. However, the expression and function of circRNAs in cervical cancer (CC) have rarely been explored. The aim of this study was to investigate the biological function of circRNA8924 in CC and elucidate the possible molecular mechanism involved. Methods: Quantitative polymerase chain reaction was used to determine mRNA expression of circRNA8924, miR-518d-5p/519-5p and CBX8 in CC tissues and cells. CBX8 protein expression was measured by Western blotting. The CCK-8 assay was used to evaluate cell proliferation, and the transwell assay to determine cell migration and invasion. The luciferase reporter assay was used to determine the direct regulation of miR-518d-5p/519-5p and circRNA8924 or CBX8 Results: The study demonstrated that the expression level of circRNA8924 in CC was significantly higher than that in the adjacent normal tissues (P < 0.001), and that it was also associated with tumor size, FIGO staging and myometrial invasion. The knockdown of circRNA8924 significantly inhibited the proliferation, migration and invasion of CC cells SiHa and HeLa. The expression level of miR-518d-5p/519-5p was negatively correlated with circRNA8924, and circRNA8924 regulated CBX8 by competitively binding to miR-518d-5p/519-5p. Conclusions: CircRNA8924 is highly expressed in CC tissue and can be considered a competitive endogenous RNA of the miR-518d-5p/519-5p family to promote the malignant biological behavior of CC cells. It is suggested that it may serve as a new biomarker for CC diagnosis and disease progression and provide potential targets for targeted therapy.

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Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8

Liu

Wang

Long

et al. 2018

Cell Physiol Biochem

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Section: Introductionmentioning

confidence: 99%

“…Recently much attention has been paid to Circular RNAs (circRNAs), which is one of a class of noncoding RNAs, with circular structure by joining the 3′end of the RNA to the 5′end. 4 They were important in regulating the expression of other genes. For example, Liu et al found 2:27713879|27755789 and 2:240822115| 240867796 participated in regulation network in osteogenic differentiation.…”

mentioning

confidence: 99%

CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

Yin

Wang

et al. 2018

J Cellular Molecular Medi

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ObjectiveWe aimed to discover the molecular mechanism of hsa_circ_0076694 (circRUNX2) on osteogenic differentiation. We also explored the interaction between circRUNX2, miR‐203 and RUNX2.MethodsClinical samples obtained from femoral neck fracture patients’ bone tissues were used to collect circRUNX2, miR‐203, and RUNX2 expression data, while their expression changes were observed in human bone mesenchymal stem cells (hBMSCs) during osteogenic differentiation. QRT‐PCR and Western blot were used to analyse levels of RNAs and proteins. Biotin pull down, RIP, RNA FISH, and Dual‐Luciferase Reporter assays demonstrated the relationship between circRUNX2, miR‐203, and RUNX2. ALP and ARS staining were used to measure the degree of osteogenic differentiation under the control of circRUNX2, miR‐203.ResultsCircRUNX2 were down‐regulated in osteoporotic patients’ bone tissues. CircRUNX2 could inhibit miR‐203 expression by sponging miR‐203. MiR‐203 inhibited osteogenic differentiation by targeting the 3′‐UTR of RUNX2 and down‐regulate RUNX2 expression. Overexpression of circRUNX2 promoted the expression of osteogenic differentiation‐related proteins such as RUNX2, OCN, OPN, BSP, and prevented osteoporosis.ConclusioncircRUNX2 could sponge miR‐203 and enhance RUNX2 expression, thus circRUNX2 prevents osteoporosis and may provide a novel therapeutic strategy for it.

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“…The stability of circRNA partly contributes to their abundant expression. It is indicated that the expression level of some circRNAs is higher than that of their corresponding linear mRNAs [42]. Therefore, circRNAs seem to possess distinct advantages as candidate biomarkers for cancer diagnosis.…”

Section: Discussionmentioning

confidence: 99%

A Meta-Analysis of the Diagnostic Accuracy of Circular RNAs in Digestive System Malignancy

Chen¹,

Zhang²,

Han³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs), a novel class of noncoding RNAs, have been found to be dysregulated in various cancers. However, the clinical application value of these circRNAs in digestive system cancers remains to be clarified. We aimed to comprehensively explore the potential role of circRNAs as diagnostic indicators in digestive system malignancies. Methods: Relevant studies were systematically retrieved from PubMed, Web of Science and the Cochrane Library. The data that were required to complete 2 × 2 contingency tables were obtained from the included studies. Stratified analyses by cancer type, sample size and publication year were performed. Results: Thirteen studies with 2,276 individuals were included in the meta-analysis. The pooled sensitivity and specificity of circRNAs in the diagnosis of digestive system malignancy were 0.72 [95% confidence interval (CI): 0.65-0.77] and 0.77 (95% CI: 0.72-0.81), respectively. The overall positive likelihood ratio was 3.09 (95% CI: 2.64-3.62), and the overall negative likelihood ratio was 0.37 (95% CI: 0.31-0.44). The pooled diagnostic odds ratio was 8.38 (95% CI: 6.86-10.25), and the overall area under the curve was 0.81 (95% CI: 0.77-0.84), indicating good discriminative ability of circRNAs as biomarkers for digestive system malignancy. Conclusion: circRNAs distinguish patients with digestive system cancer from controls with relatively high diagnostic accuracy. circRNAs may be used as potential biomarkers for the diagnosis of digestive system malignancy.

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Circular RNAs in cancer: an emerging key player

Cited by 243 publications

References 56 publications

CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8

CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8

CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

A Meta-Analysis of the Diagnostic Accuracy of Circular RNAs in Digestive System Malignancy

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