Circulating endothelial cell count, plasma vWF and soluble ICAM-1 levels following primary or elective percutaneous coronary intervention

Level of Adenosine Diphosphate Receptor P2Y12 Blockade During Percutaneous Coronary Intervention Predicts the Extent of Endothelial Injury, Assessed by Circulating Endothelial Cell Measurement

“…These observations are consistent with previous reports of an association between these clinical factors and reduced responsiveness to antiplatelet agents (31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42). In addition, diabetes has already been associated with high numbers of CEC (43).…”

“…No significant difference in preprocedural CEC counts was observed between GR and HTPR patients (8.3 Ϯ 8.8 cells/ml vs. 6.8 Ϯ 8 cells/ml, respectively; p ϭ 0.3). Consistent with the inclusion criteria limited to patients with stable CAD, these baseline values were low and within expected normal range (31). In both groups of patients, PCI induced a significant rise in CEC levels detectable 6 h after the procedure compared with pre-procedural values (GR and HTPR groups: 26.9 Ϯ 22.5 cells/ml vs. 8.3 Ϯ 8.8 cells/ml, p Ͻ 0.0001 and 59.6 Ϯ 27.5 cells/ml vs. 6.8 Ϯ 8 cells/ml, p 0.0001, respectively).…”

Role of Endothelial Progenitor Cell Mobilization After Percutaneous Angioplasty Procedure

“…A following study gave opposite results on the extent of endothelial injury induced by PCI in acute ST-elevation myocardial infarction (STEMI) or in SA [131]. In STEMI, after PCI a substantial increase in CEC count was observed (significant peak at 24h), with a positive correlation with baseline creatine kynase MB, an index of tissue necrosis, in accordance with a pioneer study by Mutin [132] that examined CEC counts in STEMI patients with fibrinolysis, detecting a very similar kinetics.…”

Circulating endothelial cells in coronary artery disease and acute coronary syndrome

“…Bethel et al [70] CD146 þ , CD45 À , CD105 þ , and DAPI þ IB/FM (CS) CD146 þ , CD45 À , vWF þ , and DAPI þ Fluid phase biopsy Bonello et al [49] CD146 þ IB Boos et al [10] CD146 þ , UEL, and size 10-50 μm IB/FM Boos et al [27] CD146 þ , UEL, and size 10-50 μm IB/FM Boos et al [62] CD146 þ , UEL, and size 10-50 μm IB/FM Boos et al [63] CD146 þ , UEL, and size 10-50 μm IB/FM Boos et al [26] CD146 þ , UEL, and size 10-50 μm IB/FM Damani et al [69] CD146 þ , CD45 À , CD105 þ , and DAPI þ IB/FM (CS) Dignat-George et al [77] CD146 þ IB Freestone et al [25] CD146 þ and size 420 μm IB/FM Hladovec et al [78] Size and shape Serial centrifugation Lampka et al [79] CD146 þ , CD45 À , and CD31 þ Flow cytometry Lee et al [29] CD146 þ and size 20-50 μm IB/FM Lee et al [32] CD146 þ and size 20-50 μm IB/FM Li et al [66] CD146 þ , CD45 À , Hoechst 33342 þ , CD31 þ , and CD133 À FWD/SSC Flow cytometry Makin et al [24] CD146 þ and size 420 μm IB/FM Mutin et al [17] CD146 þ , size 20-50 μm IB/FM Mourino-Alvarez et al [64] CD45 À , CD31 bright , CD34 þ , CD 133 À , and FWD/SSC Flow cytometry Quilici et al [67] CD146 þ and size 20-50 μm IB/FM Vargová et al [80] CD146 þ and size 20-50 μm IB/FM Wang et al [81] CD146 þ , CD31 þ , vWF þ , and size 20-50 μm IB/EM DAPI, nuclear staining of AT-rich regions; CS, CellSearch s platform; UEL, Ulex Europeus Lectin; IB, immunobead isolation; FM, fluorescence microscopy; EM, electron microscopy; FWD/SSC, size and density by forward scatter and side scatter. [4,6,44].…”