Circulating Endothelial Microparticles in Diabetes Mellitus

Tramontano, Anthony F; Lyubarova, Radmila; Tsiakos, J.; Palaia, Thomas; DeLeon, Joshua; Ragolia, Louis

doi:10.1155/2010/250476

Cited by 123 publications

(120 citation statements)

References 35 publications

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“…These results complement and extend the important findings of Nioks Werner et al [7], who demonstrated that apoptotic EMP are involved in the pathogenesis of ED in CAD. A previous study showed that a CD62E + /CD31 + ratio ≤ 1.0 suggests that endothelial cell apoptosis plays the main role in the vessel damage associated with CAD [8]. Here, we showed that the CD62E + / CD31 + ratio in the CAD group was <1.0; significantly lower than that in the healthy group (p<0.001).…”

Section: Discussionsupporting

confidence: 41%

Increased Circulating Apoptotic CD31+/CD42b- and Activated CD62E+ Endothelial Micro Particles in Coronary Artery Disease

Hu¹,

Zhang

Zhang³

et al. 2013

J Hypertens

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Section: Discussionsupporting

confidence: 41%

Increased Circulating Apoptotic CD31+/CD42b- and Activated CD62E+ Endothelial Micro Particles in Coronary Artery Disease

Hu¹,

Zhang

Zhang³

et al. 2013

J Hypertens

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“…Type 2 diabetes is especially a chronic inflammatory disease and circulating microvesicles seem to be elevated in these patients [36,37]. It was, therefore, relevant to measure inflammation parameters and microvesicle numbers in patients with diabetes who manifest increased susceptibility to air pollution [29].…”

Section: Discussionmentioning

confidence: 99%

Air pollution‐associated procoagulant changes: the role of circulating microvesicles

Emmerechts

Jacobs

kerckhoven

et al. 2012

Journal of Thrombosis and Haemostasis

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Summary. Background: Epidemiological studies suggest an association between exposure to particulate matter (PM) in air pollution and the risk of venous thromboembolism (VTE). Objectives: To investigate the underlying pathophysiological pathways linking PM exposure and VTE. Patients and methods: We assessed potential associations between PM exposure and coagulation and inflammation parameters, including circulating microvesicles, in a group of 233 patients with diabetes. Results: The numbers of circulating blood platelet‐derived and annexin V‐binding microvesicles were inversely associated with the current levels of PM2.5 or PM10, measured on the day of sampling. Recent past exposure to PM10, up to 1 week prior to blood sampling, estimated at the patients’ residential addresses, was associated with elevated high‐sensitivity C‐reactive protein (CRP), leukocytes and fibrinogen, as well as with tissue factor (TF)‐dependent procoagulant changes in thrombin generation assays. When longer windows of past exposure were considered, up to 1 year preceding blood sampling, procoagulant changes were evident from the strongly increased numbers of red blood cell‐derived circulating microvesicles and annexin V‐binding microvesicles, but they no longer associated with TF. Past PM exposure was never associated with activated partial thromboplastin time (aPTT), prothrombin time (PT), or factor (F) VII, FVIII, FXII or D‐dimers. Residential distance to a major road was only marginally correlated with procoagulant changes in FVIII and thrombin generation. Conclusions: Increases in the number of microvesicles and in their procoagulant properties, rather than increases in coagulation factors per se, seem to contribute to the risk of VTE, developing during prolonged exposure to air pollutants.

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“…T2DM was used as the disease model because of the high risk for vascular complications, and previous works have reported the pathological implications 33,34 and clinical utilities 35,36 of circulating MPs in T2DM patients. Interestingly, both immune MPs (LMPs and NMPs) were strongly correlated to established cardiovascular risk factors, including body mass index, carotid intima-media thickness and triglyceride levels (Po0.05, Po0.01 and Po0.001, respectively) in T2DM patients (Figure 5d), suggesting a link between inflammation and lipid metabolism in diabetes patients.…”

Section: Mps Separation Using Hidffmentioning

confidence: 99%

Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation

et al. 2017

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Efficient separation of sub-micrometer synthetic or biological components is imperative in particle-based drug delivery systems and purification of extracellular vesicles for point-of-care diagnostics. Herein, we report a novel phenomenon in spiral inertial microfluidics, in which the particle transient innermost distance (D inner ) varies with size during Dean vortices-induced migration and can be utilized for small microparticle (MP) separation; aptly termed as high-resolution Dean flow fractionation (HiDFF). The developed technology was optimized using binary bead mixtures (1-3 μm) to achieve~100-to 1000-fold enrichment of smaller particles. We demonstrated tunable size fractionation of polydispersed drug-loaded poly(lactic-co-glycolic acid) particles for enhanced drug release and anti-tumor effects. As a proof-of-concept for microvesicles studies, circulating extracellular vesicles/ MPs were isolated directly from whole blood using HiDFF. Purified MPs exhibited well-preserved surface morphology with efficient isolation within minutes as compared with multi-step centrifugation. In a cohort of type 2 diabetes mellitus subjects, we observed strong associations of immune cell-derived MPs with cardiovascular risk factors including body mass index, carotid intima-media thickness and triglyceride levels (Po0.05). Overall, HiDFF represents a key technological progress toward highthroughput, single-step purification of engineered or cell-derived MPs with the potential for quantitative MP-based health profiling. NPG Asia Materials (2017) 9, e434; doi:10.1038/am.2017.175; published online 29 September 2017 INTRODUCTIONEnabling technologies for continuous, size-based separation of submicrometer engineered or biological components are highly desirable in clinical applications, such as particle-based drug delivery systems 1 and the purification of extracellular vesicles in clinical diagnostics. 2 In microparticle fabrication, conventional 'bottom-up' self-assembly emulsification techniques yield a broad particle size distribution, which can affect the drug release kinetics and biotransport in blood. 3,4 Although well-controlled and monodisperse particles can be produced by 'top-down' approaches using specific lithographic techniques 5,6 and microfluidics, 7,8 microfabricated particles are prone to damage during mechanical harvesting, a problem further aggravated at the smaller/nanoscale level. Similarly, microfluidic synthesis of drug-loaded polymeric particles requires compatible drug/surfactant chemistry with additional steps to remove solvent prior use. Developing novel tools to achieve tunable size fractionation of polydispersed synthetic particles would enable optimal biodistribution and controlled drug release. Such technologies also facilitate physical isolation of smaller biological targets (o2 μm) including platelets, microbes and extracellular vesicles in a label-free manner for unbiased downstream analysis.

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Circulating Endothelial Microparticles in Diabetes Mellitus

Cited by 123 publications

References 35 publications

Increased Circulating Apoptotic CD31+/CD42b- and Activated CD62E+ Endothelial Micro Particles in Coronary Artery Disease

Increased Circulating Apoptotic CD31+/CD42b- and Activated CD62E+ Endothelial Micro Particles in Coronary Artery Disease

Air pollution‐associated procoagulant changes: the role of circulating microvesicles

Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation

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