Circulating MicroRNAs as Promising Biomarkers in Forensic Body Fluids Identification

Dumache, Raluca; Ciocan,; Muresan, Camelia; Af, Rogobete; Enache, Alexandra

doi:10.7754/clin.lab.2015.150207

Cited by 22 publications

(20 citation statements)

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“…Besides the diagnostic potential of circulatory miRNAs in AD and other various other disease, they also characterized as important candidate molecules in forensic bioanalytical procedures due to their tiny size and much more stability and specificity [100–101]. Important body fluids in forensic settings are blood, saliva, semen, vaginal secretion and menstrual blood, where different miRNAs are expressed differently eg.…”

Section: Critical Analysis and Discussion Of Circulatory Mirna Studiesmentioning

confidence: 99%

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Kumar

Reddy

2016

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

248

181

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Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by memory loss, multiple cognitive abnormalities and intellectual impairments. Currently, there are no drugs or agents that can delay and/or prevent the progression of disease in elderly individuals, and there are no peripheral biomarkers that can detect AD early in its pathogenesis. Research has focused on identifying biomarkers for AD so that treatment can be begun as soon as possible in order to restrict or prevent intellectual impairments, memory loss, and other cognitive abnormalities that are associated with the disease. One such potential biomarker is microRNAs that are found in circulatory biofluids, such as blood and blood components, serum and plasma. Blood and blood components are primary sources where miRNAs are released in either cell-free form and then bind to protein components, or are in an encapsulated form with microvesicle particles. Exosomal miRNAs are known to be stable in biofluids and can be detected by high throughput techniques, like microarray and RNA sequencing. In AD brain, enriched miRNAs encapsulated with exosomes crosses the blood brain barrier and secreted in the CSF and blood circulations. This review summarizes recent studies that have identified miRNAs in the blood, serum, plasma, exosomes, cerebral spinal fluids, and extracellular fluids as potential biomarkers of AD. Recent research has revealed only six miRNAs–miR-9, miR-125b, miR-146a, miR-181c, let-7g-5p, and miR-191-5p – that were reported by multiple investigators. Some studies analyzed the diagnostic potential of these six miRNAs through receiver operating curve analysis which indicates the significant area-under-curve values in different biofluid samples. miR-191-5p was found to have the maximum area-under-curve value (0.95) only in plasma and serum samples while smaller area-under-curve values were found for miR-125, miR-181c, miR-191-5p, miR-146a, and miR-9. This article shortlisted the promising miRNA candidates and discussed their diagnostic properties and cellular functions in order to search for potential biomarker for AD.

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Section: Critical Analysis and Discussion Of Circulatory Mirna Studiesmentioning

confidence: 99%

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Kumar

Reddy

2016

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

248

181

View full text Add to dashboard Cite

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“…Furthermore, some chemical and catalytic assays may suffer from poor sensitivity or specificity, and although immunological assays have been exploited as confirmatory tests, there are still reports of false indications in certain situations [3,4]. Alternative methods have been developed recently using the tissue-specific expression of mRNA and miRNA [5,6]. The intrinsic instability of RNA limits their applications [7,8].…”

Section: Introductionmentioning

confidence: 98%

Novel identification of biofluids using a multiplex methylation sensitive restriction enzyme-PCR system

Lin

Tsai

Lee

et al. 2016

Forensic Science International: Genetics

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“…Additionally, circulating miRNAs could be useful as diagnostic or/and prognostic biomarkers for various cardiovascular diseases, such as atrial fibrillation, myocardial infarction (MI), heart failure and cardiac hypertrophy [5,6,[10][11][12][13][14][15][16]. Interestingly, miRNAs have been found in plasma and other body fluids as stable molecules protected from endogenous RNase activity, which suggests that circulating miRNAs might be ideal biomarkers in cardiovascular diseases [17,18].…”

Section: Introductionmentioning

confidence: 99%

Plasma MicroRNA-126-5p is Associated with the Complexity and Severity of Coronary Artery Disease in Patients with Stable Angina Pectoris

Zhao

Liu

et al. 2016

Cell Physiol Biochem

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Background: Coronary artery disease (CAD) is a major problem worldwide. As an endothelium-enriched microRNA (miRNA), miR-126 has been reported to serve as a potential biomarker of acute myocardial infarction. However, the relationship between miR-126 and the severity of CAD remains unknown. This study was designed to test whether circulating miR-126 levels are associated with the severity of CAD. Methods: The present study enrolled 40 patients who had risk factors for CAD without angiographically significant CAD, and 110 patients presenting with stable angina pectoris, who were validated left main coronary artery disease (LMCA) and/or multi-vessel disease by coronary angiography. The expression levels of plasma miR-126-5p from all enrolled subjects were estimated by quantitative real-time polymerase chain reaction (qRT-PCR). Then, the relationships between plasma miR-126-5p levels, number of diseased vessels and the corresponding Synergy between PCI with Taxus and Cardiac surgery (SYNTAX) score were analyzed. Results: The expression of circulating miR-126-5p was affected by some CAD risk factors including aging, dyslipidemia and DM. Furthermore, plasma miR-126-5p levels were significantly down-regulated in CAD patients with multi-vessel disease, higher SYNTAX score, rather than isolated LMCA and low SYNTAX score. Conclusion: Circulating miR-126-5p has emerged as a potential biomarker for complexity and severity of CAD in patients with stable angina pectoris.

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Circulating MicroRNAs as Promising Biomarkers in Forensic Body Fluids Identification

Cited by 22 publications

References 0 publications

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Are circulating microRNAs peripheral biomarkers for Alzheimer's disease?

Novel identification of biofluids using a multiplex methylation sensitive restriction enzyme-PCR system

Plasma MicroRNA-126-5p is Associated with the Complexity and Severity of Coronary Artery Disease in Patients with Stable Angina Pectoris

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