Circulating p53 and cytochrome c levels in acute myocardial infarction patients

Dinçer, Yıldız; Himmetoglu, Solen; Bozçalı, Evin; Vural, Vural Ali; Akçay, Tülay

doi:10.1007/s11239-009-0328-0

Cited by 6 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore in present study, we investigated 8-OHdG which is one of the most abundant oxidative DNA products and a sensitive DNA damage marker and found increased oxidative DNA damage in the serum of patients with AMI, so oxidative stress is also a condition under which DNA damage occurs. In agreement with previous studies, our results showed an increase in p53 and 8-OHdG in the circulation [23,24]. Furthermore, the bcl-2 gene family is a complex network regulating apoptosis which some of the candidates can suppress apoptosis while the others can induce it [50].…”

Section: Discussionsupporting

confidence: 93%

“…Serum levels of p53 and 8-OHdG were measured in the patients and healthy individuals with competitive ELISA kits from Bender MedSystems (Burlingame, CA, USA) [23] and GENTAUR (8-OHdG Check; Japan Institute for the Control of Aging, Fukuroi, Shizuoka, Japan) [29] respectively. Measurement was performed according to the manufacturer's instructions.…”

Section: P53 and 8-ohdg Measurement By Elisa Analysismentioning

confidence: 99%

“…The most reliable serum markers of apoptosis and oxidative DNA damage are p53 and 8-OHdG respectively [23,24]. Evidence of apoptosis is provided by p53-stimulated transcription of the proapoptotic bax gene [25][26][27], and repression of the antiapoptotic bcl-2 gene [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The role of p53, bax, bcl2, and 8-OHdG in human acute myocardial infarction

et al. 2010

View full text Add to dashboard Cite

Apoptosis is implicated in unfavorable remodeling of the left ventricle during acute myocardial infarction (AMI). Both DNA damage and p53 play important roles in regulating apoptosis. Expression patterns of apoptotic regulating genes such as p53, bax, and bcl-2 highlight the importance of inhibiting ventricle remodeling and subsequent injuries. In the present study, serum levels of p53 and 8-hydroxy-2-deoxyguanosine (8-OHdG) as well as p53, bax, and bcl-2 expression were examined after the onset of AMI in Iranian patients. Serum levels of p53 and 8-OHdG were measured by enzyme-linked immunosorbent assay (ELISA) and the presence of p53 protein and mRNA expression of p53, bax, and bcl-2 were analyzed by Western blotting and real time RT-PCR methods respectively. In patients presenting with AMI, serum levels of p53 and 8-OHdG were increased in comparison to healthy controls. Likewise, transcripts of p53 and bax were also elevated in patients while bcl-2 was decreased. Collectively, our data suggest the novel use of p53 and 8-OHdG as markers of apoptosis and DNA damage following AMI. Our results also revealed that apoptosis occurs in concert with an up-regulation of p53 and bax and a down-regulation of bcl-2 which may suggest a possible therapeutic intervention in patients recovering from AMI.© Versita Sp. z o.o.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: P53 and 8-ohdg Measurement By Elisa Analysismentioning

confidence: 99%

See 1 more Smart Citation

The role of p53, bax, bcl2, and 8-OHdG in human acute myocardial infarction

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Experimental simulation of myocardial ischemia showed that the apoptotic process is mediated by a well-known protein p53 (2). An involvement of the protein p53 in CAD is also supported by human observation showing the elevated cell-free protein p53 in patients after myocardial infarction (3).…”

Section: Introductionmentioning

confidence: 75%

TP53 rs1042522 and rs8064946 variants in myocardial infarction

Hakobjanyan¹,

Stahelova²,

Mrázek³

et al. 2019

BLL

View full text Add to dashboard Cite

OBJECTIVE: This study investigated the hypothesis that the single nucleotide polymorphisms (SNPs) of TP53 gene are related to a risk of myocardial infarction. METHODS: The coding SNP at codon 72 (rs1042522) and non-coding rs8064946 SNP were genotyped by polymerase chain reaction with sequence specifi c primers in 205 Czech patients with myocardial infarction and 148 Czech control subjects. RESULTS: The distribution of both SNPs was in agreement with the Hardy-Weinberg equilibrium and was similar to other European populations. Our power analysis showed 96 % of probability to detect an odd ratio equal to 2. Neither rs1042522 nor rs8064946 were associated with the risk of myocardial infarction. The haplotypes combined of rs1042522 and rs8064946 were not associated with myocardial infarction in the present study. CONCLUSION: The TP53 SNPs are not strongly associated with genetic predisposition to myocardial infarction

show abstract

“…These authors also observed a time-dependent decrease in p53 levels. Such a relationship could suggest, that p53 may be a marker of apoptosis after myocardial infarction [14]. In addition, it was demonstrated that expression of proapoptotic genes including p53 was higher in unstable than in stable atherosclerotic plaques [15].…”

Section: Discussionmentioning

confidence: 95%