Circulation of Chikungunya virus in Aedes aegypti in Maranhão, Northeast Brazil

Aragão, Carine Fortes; Cruz, Ana Cecília Ribeiro; Neto, Joaquim Pinto Nunes; Monteiro, Hamilton Antônio de Oliveira; Silva, Eliana Vieira Pinto da; Silva, Sandro Patroca da; Andrade, Aylane Tamara dos Santos; Tadei, Wanderli Pedro; Pinheiro, Valéria Cristina Soares

doi:10.1016/j.actatropica.2018.06.022

Cited by 18 publications

(17 citation statements)

References 12 publications

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“…aegypti occurs even in the months of low rainfall, due to the residents' habit to store water for domestic activities in large deposits, such as tanks and barrels, providing continuous availability of breeding sites. This vector's behavior has been reported in other studies performed in the municipalities of the interior of Maranhão [31][32][33]35,36].…”

Section: Discussionsupporting

confidence: 79%

“…Recently, CHIKV was detected in Ae. aegypti in two municipalities of Maranhão, Caxias and São Mateus do Maranhão [36], corroborating the clinical data of the occurrence of this arbovirus in the region. In this study, we investigated the natural infection by DENV, ZIKV, and CHIKV in Ae.…”

Section: Introductionsupporting

confidence: 77%

“…As this study was carried out in residences located in urban neighborhoods, few specimens were collected. However, it is necessary to monitor their density, as this species have been showing changes in behavior and were reported close to urban areas [64,65], including studies conducted in the municipality of Caxias, Maranhão [35,36].…”

Section: Discussionmentioning

confidence: 99%

“…CHIKV had already been detected in Ae. aegypti in the municipalities of Caxias and São Mateus do Maranhão, in an investigation performed during the first outbreak that occurred in the state in 2016, however, no information on the circulating genotype was available at that time [36].…”

Section: Discussionmentioning

confidence: 99%

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Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Aragão

Pinheiro

Neto

et al. 2019

Viruses

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“…aegypti vector that is abundant throughout Brazil [71]. In line with this, CHIKV-ECSA was recently detected in Aedes aegypti from Maranhão [72] and Rio de Janeiro states [73].…”

Section: Discussionmentioning

confidence: 70%

Chikungunya virus outbreak in the Amazon region: replacement of the Asian genotype by an ECSA lineage

Naveca

Claro

Giovanetti

et al. 2018

Preprint

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48 49 50 51 52 Corresponding authors: luiz.alcantara@ioc.fiocruz.br and nuno.faria@zoo.ox.ac.uk 3 53 Abstract 54 Background 55 Since its first detection in the Caribbean in late 2013, chikungunya virus (CHIKV) has 56 affected 51 countries in the Americas. The CHIKV epidemic in the Americas was 57 caused by the CHIKV-Asian genotype. In August 2014, local transmission of the 58 CHIKV-Asian genotype was detected in the Brazilian Amazon region. However, a 59 distinct lineage, the CHIKV-East-Central-South-America (ECSA)-genotype, was 60 detected nearly simultaneously in Feira de Santana, Bahia state, northeast Brazil. The 61 genomic diversity and the dynamics of CHIKV in the Brazilian Amazon region 62 remains poorly understood despite its importance to better understand the 63 epidemiological spread and public health impact of CHIKV in the country. 64 65 Methodology/Principal Findings 66 We report a large CHIKV outbreak (5,928 notified cases between August 2014 and 67 August 2018) in Boa vista municipality, capital city of Roraima's state, located in the 68 Brazilian Amazon region. In just 48 hours, we generated 20 novel CHIKV-ECSA 69 genomes from the Brazilian Amazon region using MinION portable genome 70 sequencing. Phylogenetic analyses revealed that despite an early introduction of the 71 Asian genotype in 2015 in Roraima, the large CHIKV outbreak in 2017 in Boa Vista 72 was caused by an ECSA-lineage most likely introduced from northeastern Brazil. 73 Epidemiological analyses suggest a basic reproductive number of R 0 of 1.66, which 74 translates in an estimated 39 (95% CI: 36 to 45) % of Roraima's population infected 75 with CHIKV-ECSA. Finally, we find a strong association between Google search 76 activity and the local laboratory-confirmed CHIKV cases in Roraima. 77 78 Conclusions/Significance 79 This study highlights the potential of combining traditional surveillance with portable 80 genome sequencing technologies and digital epidemiology to inform public health 81 surveillance in the Amazon region. Our data reveal a large CHIKV-ECSA outbreak in 82 Boa Vista, limited potential for future CHIKV outbreaks, and indicate a replacement 83 of the Asian genotype by the ECSA genotype in the Amazon region. 84 85 4 86 Author Summary 87 Until the end of 2017, Brazil notified the highest number of infections caused by 88 chikungunya virus (CHIKV) in the Americas. We investigated a large CHIKV 89 outbreak in Boa vista municipality in the Brazilian Amazon region. Rapid portable 90 genome sequencing of 20 novel isolates and subsequent genetic analysis revealed that 91 ECSA lineage was introduced from northeastern Brazil to Roraima around July 2016. 92 Epidemiological analyses suggest a basic reproductive number of R 0 of 1.66, which 93 suggests that approximately 39% of Roraima's population was infected with CHIKV-94 ECSA. Given the dominance of the CHIKV-Asian genotype in the Americas, our data 95 highlights the rapid spread of a less understood and poorly characterized CHIKV-96 ECSA genotype in Brazil. Investigations on p...

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Genetic characterization of Chikungunya virus circulating in individuals from Paraná, Brazil

et al. 2022

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Circulation of Chikungunya virus in Aedes aegypti in Maranhão, Northeast Brazil

Cited by 18 publications

References 12 publications

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Chikungunya virus outbreak in the Amazon region: replacement of the Asian genotype by an ECSA lineage

Genetic characterization of Chikungunya virus circulating in individuals from Paraná, Brazil

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