Citrate Metabolism in
            <i>Aerobacter cloacae</i>

O'Brien, R.W.; Geisler, Jarmila

doi:10.1128/jb.119.3.661-665.1974

Cited by 11 publications

(13 citation statements)

References 21 publications

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“…Growth of the organism. E. cloacae NCTC 10005 was grown on Na+ citrate anid K+ citrate medium as described by O'Brien and Geisler (10). Anaerobic cultures were grown in 1-liter bottles filled to capacity with medium rendered anaerobic by the addition of sterile sodium dithionite.…”

Section: Methodsmentioning

confidence: 99%

“…Preparation of cell extracts and enzyme assays. The frozen cells were thawed in 0.05 M tris(hydroxymethyl)aminomethane-chloride buffer, pH 7, and extracts were prepared according to the method of O'Brien and Geisler (10).…”

Section: Methodsmentioning

confidence: 99%

“…In the case of K. aerogenes, NCTC 418, Na+ is essential for the anaerobic growth of the organism (12) and for the activation of the OAA decarboxylase (14). Anaerobic growth of A. cloacae, on the other hand, is independent of Na + and the OAA decarboxylase does not require any metal ion activator (10).…”

mentioning

confidence: 99%

“…The aerobic catabolism of citrate by both K. aerogenes and A. cloacae proceeds via the citric acid cycle and is independent of Na+ (8,10,15). Under aerated conditions (defined as those under which no dissolved oxygen could be measured in the medium even though the culture was aerated), K. aerogenes metabolized citrate via the citric acid cycle in the absence of Na+.…”

mentioning

confidence: 99%

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Induction of citrate lyase in Enterobacter cloacae grown under aerated conditions and its effect on citrate metabolism

O'Brien¹

1975

J Bacteriol

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Growth of Enterobacter cloacae on K+ citrate under aerated conditions (no detectable oxygen tension in the medium even though it was aerated) was slower (mean generation time, 130 min) than under aerobic conditions (mean generation time, 72 min), but with a faster utilization of citrate, resulting in a molar growth yield of 10.6 g (dry weight) of cells per mol of citrate utilized versus 40 g (dry weight) of cells per mol of citrate utilized for aerobic growth. The rapid utilization of citrate under aerated conditions was apparently due to the induction of citrate lyase and was supported by the finding that cells excreted acetate and a small amount of oxalacetate under aerated conditions, but not under aerobic conditions when the cells were devoid of citrate lyase activity. The activity of oxalacetate decarboxylase in aerated cells was slightly lower than in aerobic cells, indicating that little of the oxalacetate produced by the citrate lyase was metabolized by the decarboxylase. Oxalacetate was probably metabolized by malate dehydrogenase, previously shown to be present in anaerobic and aerobic cells. Thus, about 70% of the citrate was cleaved by the citrate lyase, resulting in little or no production of energy for growth. The remaining citrate was metabolized via the citric acid cycle under aerated conditions, since the cells contained a-ketoglutarate dehydrogenase at the same level as in aerobically grown cells. The presence of the other enzymes of the cycle was shown in earlier studies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Induction of citrate lyase in Enterobacter cloacae grown under aerated conditions and its effect on citrate metabolism

O'Brien¹

1975

J Bacteriol

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“…Most of the enterobacteria which are able to utilize citrate possess an inducible citrate transport system under aerobic as well as under anaerobic conditions [5,6,[85][86][87][88]98,99]. Bacteria like E. aerogenes, Aerobacter indologenes, A. cloacae, Proteus rettgeri and Klebsiella pneumoniae, therefore, are able to utilize citrate aerobically as well as anaerobically.…”

Section: Citrate Metabolism In Enteric Bacteriamentioning

confidence: 99%

Citrate metabolism in anaerobic bacteria

Antranikian

Giffhorn

1987

FEMS Microbiology Letters

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The regulation of anaerobic citrate metabolism is very diverse among different groups of bacteria. In organisms like Streptococcus lactis and Clostridium sporosphaeroides which lack citrate synthase, the activity of its antagonistic enzyme, citrate lyase, need not be regulated. Many anaerobes like Rhodocyclus gelatinosus and Clostridium sphenoides are able to synthesize their own l‐glutamate and contain citrate synthase. In these bacteria the activity of citrate metabolizing enzymes which are involved in a cascade system are under strict control. In Rc. gelatinosus activation/inactivation of citrate lyase is controlled by acetylation/deacetylation which is catalyzed by its corresponding regulatory enzymes, citrate lyase ligase and citrate lyase deacetylase. In C. sphenoides inactivation of citrate lyase is accomplished by deacetylation as well as by changing in the enzyme conformation. Activation of citrate lyase is catalyzed by citrate lyase ligase whose activity in addition is modulated by phosphorylation/dephosphorylation. Further, electron transport process also seems to play a role in the inactivation of citrate metabolizing enzymes in enteric bacteria.

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Acetylene reduction (N2-fixation) by Enterobacteriaceae isolated from industrial wastewaters and biological treatment systems

Neilson¹,

Allard²

1985

Appl Microbiol Biotechnol

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Citrate Metabolism in Aerobacter cloacae

Cited by 11 publications

References 21 publications

Induction of citrate lyase in Enterobacter cloacae grown under aerated conditions and its effect on citrate metabolism

Induction of citrate lyase in Enterobacter cloacae grown under aerated conditions and its effect on citrate metabolism

Citrate metabolism in anaerobic bacteria

Acetylene reduction (N2-fixation) by Enterobacteriaceae isolated from industrial wastewaters and biological treatment systems

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