CK-19 Expression by RT-PCR in the Peripheral Blood of Breast Cancer Patients Correlates with Response to Chemotherapy

Manhani, Ana Rita; Manhani, Reinaldo; Soares, Heloisa P.; Bendit, Israel; Lopes, Fabiana; Nicoletti, André Gustavo Bombana; Fonseca, Fernando L. A.; Novaes, Marcel; Zatta, Simone Meneghetti; Arias, Vítor; Giralt, S.; Giglio, Auro del

doi:10.1023/a:1010621901102

Cited by 19 publications

(7 citation statements)

References 12 publications

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“…We can, however, assess its rate of positivity. In this study it reached 31% (95% CI: 30-33%) which is similar to that of RT-PCR in the peripheral blood of BC patients according to several authors [24,25] and with a recent metaanalysis conducted by one of the authors [26]. It is possible, therefore, that less invasive and easier methodologies such as the detection of circulating epithelial cells in the peripheral blood of BC patients may, in the future, substitute BM IC.…”

Section: Discussionsupporting

confidence: 83%

Immunocytochemical detection of epithelial cells in the bone marrow of primary breast cancer patients: a meta-analysis

Weinschenker

Soares

Clark³

et al. 2004

Breast Cancer Res Treat

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Section: Discussionsupporting

confidence: 83%

Immunocytochemical detection of epithelial cells in the bone marrow of primary breast cancer patients: a meta-analysis

Weinschenker

Soares

Clark³

et al. 2004

Breast Cancer Res Treat

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“…The accuracy of CTC load comparison by Q-RT-PCR would depend on the degree of constancy of CK19 or other chosen marker expression over time. Indirect evidence from a study following patients during the course of chemotherapy suggests that stability of CK19 was sufficient to detect the correlation with treatment outcome [7]. Highly correlative gene expression profiles of core biopsies and resected tumors temporally spaced within days and weeks also suggest relative constancy of the marker genes within short but clinically important periods of time [21].…”

Section: Discussionmentioning

confidence: 99%

“…A multicenter prospective study compared different quantities of CTCs in relation to outcome and showed that patients with more than five CTCs per 7.5 ml of blood had a shorter progression-free and overall survival (2.7 months vs. 7.0 months and 10.1 months vs. >18 months) [6]. Noticeably decreased levels of CTCs are seen in response to chemotherapy suggesting a role for measurement of CTCs in treatment monitoring [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection of circulating epithelial cells by Q-RT-PCR

Iakovlev

Goswami

Vecchiarelli

et al. 2007

Breast Cancer Res Treat

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“…Such an example is the utilisation of tyrosinase, an enzyme present only in melanocytes, for the detection of MRD in melanoma patients (Li et al, 2000). In breast cancer patients, molecules such as CK, expressed in epithelial cells but not expressed in lymphocytes, were investigated (Kruger et al, 1996;Manhani et al, 2001;Ismail et al, 2003). Using PCR to detect nonspecific epithelial markers may yield a robust and simple assay for the detection of MRD in patients with epithelial-derived tumours.…”

Section: Discussionmentioning

confidence: 99%

Multimarker RT–PCR assay for the detection of minimal residual disease in sentinel lymph nodes of breast cancer patients

et al. 2006

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The presence of metastases in lymph nodes is the most powerful prognostic factor in breast cancer patients. Routine histological examination of lymph nodes has limited sensitivity for the detection of breast cancer metastases. The aim of the present study was to develop a multimarker reverse transcriptase -polymerase chain reaction (RT-PCR) assay for the detection of minimal residual disease in sentinel nodes of breast cancer patients. RNA was extracted from 30 sentinel lymph nodes (SLN) obtained from 28 patients, three primary breast cancers (positive controls), three lymph nodes from patients with benign diseases, and peripheral blood lymphocytes of 10 healthy volunteers (negative controls). RT -PCR was performed using the following markers; cytokeratin (CK)-19, NY-BR-1 and mammaglobin B. RT -PCR results were compared to enhanced histopathologic examination and immunohistochemistry (IHC). All three positive controls showed strong PCR amplification for all three markers. None of the 13 negative controls was amplified by any of the three markers. Among the 30 SLN analysed, breast cancer metastases were detected in six SLNs by routine histology, in eight by IHC and in 15 by RT -PCR. We conclude that a multimarker RT -PCR assay probing for NY-BR-1, mammaglobin-B, and CK-19 is more sensitive compared to enhanced pathologic examination. This method may prove to be of value in breast cancer staging and prognosis evaluation. Although up to 80% of breast cancer patients are identified at an early stage when disease is apparently localised, 30 -35% of nodenegative patients will develop clinically detectable distant metastases within 5 years of treatment of the primary breast carcinoma and ultimately suffer tumour-related mortality (Nemoto et al, 1980). Routine histological examination of lymph nodes has limited sensitivity for the detection of breast cancer metastases. It is usually performed with a single haematoxilyn and eosin (H&E)-stained section through the examined node and therefore small metastases can go undetected. In a landmark study conducted by the International (Ludwig) Breast Cancer Group (International (Ludwig) Breast Cancer Study Group, 1990), serial sectioning of axillary lymph nodes judged to be disease free after routine histological examination revealed micrometastases in 83 (9%) of 921 breast cancer patients. These patients had a poorer disease-free (P ¼ 0.003) and overall (P ¼ 0.002) survival after 5 years' median follow-up than did patients whose nodes remained negative on serial sectioning. A later study performed by the same group (Cote et al, 1999) examined lymph nodes negative for metastases on routine histology from 736 patients by serial sectioning and by immunohistochemistry (IHC) with two anticytokeratins AE-1 and CAM 5.2. Occult nodal metastases were detected by serial sectioning and H&E in 7% of the patients and by IHC in 20%. After median follow-up of 12 years, occult metastases, detected by either method, were associated with significantly poor disease-free and overall survival in pos...

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CK-19 Expression by RT-PCR in the Peripheral Blood of Breast Cancer Patients Correlates with Response to Chemotherapy

Cited by 19 publications

References 12 publications

Immunocytochemical detection of epithelial cells in the bone marrow of primary breast cancer patients: a meta-analysis

Immunocytochemical detection of epithelial cells in the bone marrow of primary breast cancer patients: a meta-analysis

Quantitative detection of circulating epithelial cells by Q-RT-PCR

Multimarker RT–PCR assay for the detection of minimal residual disease in sentinel lymph nodes of breast cancer patients

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