Classification of Lectins by Pattern Recognition Using Glyconanoparticles

Jayawardena, H. Surangi N.; Wang, Xin; Yan, Mingdi

doi:10.1021/ac402069j

Cited by 31 publications

(22 citation statements)

References 39 publications

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“…This protocol could reproducibly generate nanoparticles with predicable sizes. Similarly, in our photocoupling protocol, gold nanoparticles are first subjected to thiol/disulfide-terminated perfluoroaryl azides, and are then subjected to light activation in the presence of carbohydrates (Jayawardena et al, 2013b). With this protocol, a wide range of mono-, oligo-, and polysaccharides have been conjugated to different nanomaterials.…”

Section: Synthesis Of Glyconanomaterialsmentioning

confidence: 99%

Glyconanomaterials for biosensing applications

Hao

Neranon

Ramström

et al. 2016

Biosensors and Bioelectronics

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Nanomaterials constitute a class of structures that have unique physiochemical properties and are excellent scaffolds for presenting carbohydrates, important biomolecules that mediate a wide variety of important biological events. The fabrication of carbohydrate-presenting nanomaterials, glyconanomaterials, is of high interest and utility, combining the features of nanoscale objects with biomolecular recognition. The structures can also produce strong multivalent effects, where the nanomaterial scaffold greatly enhances the relatively weak affinities of single carbohydrate ligands to the corresponding receptors, and effectively amplifies the carbohydrate-mediated interactions. Glyconanomaterials are thus an appealing platform for biosensing applications. In this review, we discuss the chemistry for conjugation of carbohydrates to nanomaterials, summarize strategies, and tabulate examples of applying glyconanomaterials in in vitro and in vivo sensing applications of proteins, microbes, and cells. The limitations and future perspectives of these emerging glyconanomaterials sensing systems are furthermore discussed.

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Section: Synthesis Of Glyconanomaterialsmentioning

confidence: 99%

Glyconanomaterials for biosensing applications

Hao

Neranon

Ramström

et al. 2016

Biosensors and Bioelectronics

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“…Many studies have taken advantage of the change in LSPR, often resulting in a color change visible by naked eyes, as a means to study carbohydrate-mediated interactions or as a detection mechanism for sensing carbohydrates [72–75]. We employed carbohydrate-conjugated Au nanoparticles (AuNPs) to differentiate plant-legume lectins [76]. Various AuNPs were treated with lectins, and changes in LSPR were subjected to linear discriminant analysis to successfully differentiate all lectins.…”

Section: Preparation Of Glyconanomaterialsmentioning

confidence: 99%

“…For the photoinitiated carbohydrate conjugation to SNPs, a method developed in our laboratory the nanoparticles were first functionalized with PFPA-silane, after which the carbohydrates were conjugated by irradiation in the presence of carbohydrates. Using this methodology we have for example prepared carbohydrate-functionalized SNPs from different mono- and oligosaccharides [15, 76, 122–124]. …”

Section: Preparation Of Glyconanomaterialsmentioning

confidence: 99%

Glyconanomaterials: Emerging applications in biomedical research

2014

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Carbohydrates constitute the most abundant organic matter in nature, serving as structural components and energy sources, and mediating a wide range of cellular activities. The emergence of nanomaterials with distinct optical, magnetic, and electronic properties has witnessed a rapid adoption of these materials for biomedical research and applications. Nanomaterials of various shapes and sizes having large specific surface areas can be used as multivalent scaffolds to present carbohydrate ligands. The resulting glyconanomaterials effectively amplify the glycan-mediated interactions, making it possible to use these materials for sensing, imaging, diagnosis, and therapy. In this review, we summarize the synthetic strategies for the preparation of various glyconanomaterials. Examples are given where these glyconanomaterials have been used in sensing and differentiation of proteins and cells, as well as in imaging glycan-medicated cellular responses.

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“…Glyconanoparticles presenting different sugars have been shown to differentiate between a set of 4 lectins using a pattern recognition approach. 54 …”

Section: Introductionmentioning

confidence: 99%

Electrochemical synthesis of nanostructured gold film for the study of carbohydrate–lectin interactions using localized surface plasmon resonance spectroscopy

Bhattarai

Sharma

Fujikawa

et al. 2015

Carbohydrate Research

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Localized surface plasmon resonance (LSPR) spectroscopy is a label-free chemical and biological molecular sensing technique whose sensitivity depends upon development of nanostructured transducers. Herein, we report an electrodeposition method for fabricating nanostructured gold films (NGFs) that can be used as transducers in LSPR spectroscopy. The NGF was prepared by electrodepositing gold from potassium dicyanoaurate solution onto a flat gold surface using two sequential controlled potential steps. Imaging by scanning electron microscopy reveals a morphology consisting of randomly configured block-like nanostructures. The bulk refractive index sensitivity of the prepared NGF is 100 ± 2 nm RIU−1 and the initial peak in the reflectance spectrum is at 518 ± 1 nm under N2(g). The figure of merit is 1.7. In addition, we have studied the interaction between carbohydrate (mannose) and lectin (Concanavalin A) on the NGF surface using LSPR spectroscopy by measuring the interaction of 8-mercaptooctyl-α-D-mannopyranoside (αMan-C8-SH) with Concanavalin A by first immobilizing αMan-C8-SH in mixed SAMs with 3,6-dioxa-8-mercaptooctanol (TEG-SH) on the NGF surface. The interaction of Con A with the mixed SAMs is confirmed using electrochemical impedance spectroscopy. Finally, the NGF surface was regenerated to its original sensitivity by removing the SAM and the bound biomolecules. The results from these experiments contribute toward the development of inexpensive LSPR based sensors that could be useful for studying glycan–protein interactions and other bioanalytical purposes.

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Classification of Lectins by Pattern Recognition Using Glyconanoparticles

Cited by 31 publications

References 39 publications

Glyconanomaterials for biosensing applications

Glyconanomaterials for biosensing applications

Glyconanomaterials: Emerging applications in biomedical research

Electrochemical synthesis of nanostructured gold film for the study of carbohydrate–lectin interactions using localized surface plasmon resonance spectroscopy

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