Abstract. Stable isotopes in tree-ring cellulose are important
tools for climatic reconstructions even though their interpretation could be
challenging due to nonclimate signals, primarily those related to tree
aging. Previous studies on the presence of tree-age-related trends during
juvenile as well as adult growth phases in δD, δ18O, and
δ13C time series yielded variable results that are not coherent
among different plant species. We analyzed possible trends in the extracted
cellulose of tree rings of 85 larch trees and 119 cembran pine trees, i.e.,
in samples of one deciduous and one evergreen conifer species collected at
the tree line in the Alps, covering nearly the whole Holocene. The age trend
analyses of all tree-ring variables were conducted on the basis of mean
curves established by averaging the cambial-age-aligned tree series. For
cambial ages over 100 years, our results prove the absence of any
age-related effect in the δD, δ18O, and δ13C time series for both the evergreen and the deciduous conifer
species, with the only exception being larch δD. However, for lower
cambial ages, we found trends that differ for each isotope and species;
i.e., mean δ13C values in larch do not vary with aging and can
be used without detrending, whereas those in cembran pine show a juvenile
effect, and the data should be detrended. Mean δ18O values
present two distinct aging phases for both species, complicating detrending.
Similarly, mean δD values in larch change in the first 50 years, whereas
cembran pine changes between 50 and 100 years. Values for these two periods of cambial age
for δD and δ18O should be used with caution for
climatic reconstructions, ideally complemented by additional information
regarding mechanisms for these trends.