Clindamycin Resistance among Methicillin Resistant &amp;lt;i&amp;gt;Staphylococcus aureus&amp;lt;/i&amp;gt; Isolated from Human and Respective Household Swine in Greater Kabale Region—South Western Uganda

Baguma, Andrew; Musinguzi, Benson; Mpeirwe, Moses; Bazira, Joel

doi:10.4236/aid.2019.94022

Cited by 4 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Çetin et al [47] reported that iML resistance was found in 15% of S. aureus isolates. However, iML resistance was determined at a higher rate in other investigations [32,39,48,49].…”

Section: Discussionmentioning

confidence: 96%

“…Staphylococcus species are reported to be the most frequently isolated bacterial agents from mastitis cases [36][37][38]. S. aureus causes gangrenous and contagious mastitis and can also infect humans through contaminated food products or direct contact [1,2,39]. For this reason, antimicrobial agents are used in the treatment of infections caused by S. aureus in order to protect both animal and public health and to prevent economic losses in cattle breeding.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

et al. 2023

View full text Add to dashboard Cite

In this study, penicillin, oxacillin, and macrolide-lincosamide-streptogramin (MLS) resistance in S. aureus strains that were isolated from bovine mastitis cases, and human patients were investigated. Inducible clindamycin resistance (iML) was not found in 30 bovine isolates, while it was detected in 3 (10%) of 30 human isolates. MIC90 values of penicillin, oxacillin and macrolide-lincosamides (ML) were 2, 0.19, >256 µg/ml in bovine isolates and were 3, 3 and 0.19-1.5 µg/ml in human isolates, respectively. Streptogramin resistance was not found in both bovine and human isolates. Although the mecA gene was detected in all of the oxacillin resistant isolates, blaZ gene could not be detected in penicillin resistant isolates. The erm(B) gene was detected in 5 (38.6%) of 13 ML-resistant bovine isolates, and the mph(C) gene was detected in 2 (66.66%) of 3 human isolates. As a result, resistance to penicillin and oxacillin was found to be higher in human S. aureus isolates, while ML resistance was found to be higher in bovine isolates in this investigation. It was concluded that the presence of genes in extra-chromosomal elements associated to penicillin and macrolide resistance should be investigated. The data obtained from this study will contribute to the studies on antimicrobial susceptibility in the field of human and veterinary medicine.

show abstract

“…Çetin et al [47] reported that iML resistance was found in 15% of S. aureus isolates. However, iML resistance was determined at a higher rate in other investigations [32,39,48,49].…”

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Similarly, other studies also reported higher inducible clindamycin resistance in Africa. 14 , 29 , 30 …”

Section: Discussionmentioning

confidence: 99%

“…Similarly, other studies also reported higher inducible clindamycin resistance in Africa. 14,29,30 Two isolates showed resistance to oxacillin but were susceptible to cefoxitine. Cefoxitine is the best indicator for the mecA gene and this was confirmed by the molecular characterization which showed that these isolates did not harbor mecA gene.…”

mentioning

confidence: 99%

Antimicrobial Resistance and Virulence Gene Profile of Clinical Staphylococcus aureus: A Multi-Center Study from Ethiopia

Ibrahim¹,

Berhe²,

Mekuria³

et al. 2023

IDR

View full text Add to dashboard Cite

Background Staphylococcus aureus causes a wide range of infections from mild skin and soft tissue to severe life-threatening bacteremia. The pathogenicity of S. aureus infections is related to various bacterial surface components and extracellular proteins such as toxic-shock syndrome (TSS) toxin and Panton-Valentine leukocidin (PVL). In this study we determine the antimicrobial resistance of isolated strains and their virulence genes in Ethiopia. Methods A total of 190 archived S. aureus isolates from four Ethiopia Antimicrobial Resistance (AMR) Surveillance sites were analyzed. The identification of S. aureus was done by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF Biotyper) and antimicrobial susceptibility test (AST) was done using VITEK ® 2. Multiplex PCR was used to detect mecA, mecC, pvl and spa genes and super-antigens ( sea, seb, sec, seh and sej staphylococcal enterotoxins). Results A total of 172 isolates were confirmed as S. aureus , 9 (5.23%) were methicillin-resistant S. aureus (MRSA) and 163 (94.76%) were methicillin-susceptible S. aureus (MSSA). AST showed that 152 (88.4%) isolates were resistant to penicillin; 90 (52.32%) resistant to trimethoprim-sulfamethoxazole; and 45 (26.16%) resistant to tetracycline. A total of 66 (38.37%) isolates harbored at least one staphylococcal enterotoxin gene and 31 (46.96%) isolates had more than one. The most frequent enterotoxin gene encountered was seb 28 (16.28%). The TSST-1 gene was detected in 23 (13.37%). Presence of staphylococcal enterotoxin gene showed significant association with antibiotic resistance to cefoxitin, benzylpenicillin, oxacillin, erythromycin, clindamycin, tetracycline and SXT. The pvl gene was detected in 102 (59.3%) of isolates. Isolates from patients below 15 years of age showed significantly high numbers of pvl gene (P = 0.02). Presence of sej (P = 0.011) and TSST-1 (P <0.001) genes were associated with the presence of pvl gene. Conclusion In this study, isolates were highly resistant to oral antibiotics and the pvl, seb, sea and TSST-1 genes were prevalent.

show abstract

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

AL-Salihi¹,

Karim²,

Al-Bayati³

et al. 2023

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

The carriage of community-acquired methicillin-resistant S. aureus (CA-MRSA) has become a source of community-associated infections, particularly in the anterior nares of people worldwide. This study was aimed at determining the prevalence of community-acquired S. aureus (CA-SA) and CA-MRSA nasal carriage among healthy individuals of various ages in Kirkuk City and evaluating the susceptibility of isolates to various antibiotics. A total of 597 healthy individuals were recruited in the community between December 1, 2021, and December 30, 2022. Nasal swabs obtained from participants were taken to the laboratory, where bacteria were isolated and identified using phenotypic characteristics. The MRSA isolates were identified by applying the modified Kirby Bauer disc diffusion technique. The results showed that the prevalence of CA-SA and CA-MRSA nasal carriers was 16.6 and 4.5%, respectively. The average age of the participants was 33.2 years, with a male-to-female ratio of 1.1: 1. The highest resistance of the isolates was observed against oxacillin (27.3%), followed by penicillin G (24.3%), amoxicillin (15.2%), erythromycin (12.1%), and tetracycline (6.1%). There was a 9.1% resistance rate to clindamycin, rifampin, gentamycin, and ciprofloxacin. However, all CA-MRSA isolates were multi-drug resistant. However, all the isolates were sensitive (100 %) to vancomycin, linezolid, and mupirocin. The findings of the present study highlight the potential for CA-SA and CA-MRSA acquisition in this population, which may be related to antibiotic abuse or overuse as well as poor hygiene. To lessen the impact of community-associated strains of MRSA nasal carriage, this necessitates the probable need for infection prevention measures and adequate antibiotic therapy.

show abstract

Clindamycin Resistance among Methicillin Resistant <i>Staphylococcus aureus</i> Isolated from Human and Respective Household Swine in Greater Kabale Region—South Western Uganda

Cited by 4 publications

References 30 publications

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

Phenotypic and Genotypic Characterization of Macrolide-Lincosamide-Streptogramin Resistance in Staphylococcus aureus Isolates from Bovine and Human

Antimicrobial Resistance and Virulence Gene Profile of Clinical Staphylococcus aureus: A Multi-Center Study from Ethiopia

Prevalence of Methicillin-Resistant and Methicillin Sensitive Staphylococcus aureus Nasal Carriage and their Antibiotic Resistant Patterns in Kirkuk City, Iraq

Contact Info

Product

Resources

About