Clinical and Biological Significances of FBLN5 in Gastric Cancer

Abstract

Abnormal FBLN5 expression levels are related to various cancer types. This study is the first to explore its clinical and biological significances in gastric cancer (GC). We used The Cancer Genome Atlas-GC (TCGA-GC) and Gene Expression Omnibus (GEO) databases to identify the differential expression of FBLN5, and its association with clinical pathological characteristics was analyzed. A Kaplan–Meier plotter was used to calculate the impact of FBLN5 on GC patient prognosis, and the biological functions of FBLN5 were analyzed. In addition, we constructed a GC tissue microarray, and performed an immunohistochemical staining of FBLN5 to verify our findings. Western blotting was conducted simultaneously to confirm that FBLN5 was overexpressed in GC. We found that the high level of FBLN5 mRNA in GC was associated with a poor prognosis. High FBLN5 expression levels were significantly correlated with INFc and N3 lymph node metastasis. Univariate and multivariate analyses showed that FBLN5 expression levels and lymph node metastasis rate were independent risk factors related to GC patient prognosis, which can be combined to construct a nomogram to serve patients. Therefore, we believe that FBLN5 is significantly related to the poor prognosis of GC patients. FBLN5 is a valuable prognostic indicator to evaluate the prognosis of GC.

Development of a novel lipid metabolism-related model predicting the prognosis of gastric cancer and exploration the role of NPR3 in gastric cancer metastasis

“…Among these 6 genes, FBLN7, NPY1R, VTN and EPHB3 have previously been reported to be involved in GC tumor development [16][17][18][19]. X Bian et al also reported that the co-family member FBLN7 was one of the independent risk factors related to GC patient prognosis [27]. Additionally, EPHB2 was reported to be associated with intestinal phenotype of gastric cancer and indicates better prognosis by suppressing gastric cancer migration [28].…”

Expression of human dCTP pyrophosphatase 1 (DCTPP1) and its association with cisplatin resistance characteristics in ovarian cancer

“… 21 And Sangerbox 22 was utilized to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of these functional protein association networks. 1‐year survival ROC, 2‐year survival ROC, 3‐year survival ROC and Kaplan–Meier (KM) survival of OC patients were also analysed by Sangerbox tool and KM Plotter database 23,24 . Furthermore, bioinformatics and text mining performed by Coremine Medical (http://www.coremine.com/medical/#search) was used to confirm whether DCTPP1 associated with OC and drug resistance 25,26…”

“…1‐year survival ROC, 2‐year survival ROC, 3‐year survival ROC and Kaplan–Meier (KM) survival of OC patients were also analysed by Sangerbox tool and KM Plotter database. 23 , 24 Furthermore, bioinformatics and text mining performed by Coremine Medical ( http://www.coremine.com/medical/#search ) was used to confirm whether DCTPP1 associated with OC and drug resistance. 25 , 26…”