Clinical Efficacy of Macrophage-Activating Chinese Mixed Herbs (MACH) in Improvement of Embryo Qualities in Women with Long-Term Infertility of Unknown Etiology

Abstract

Despite the recent technological advances in in vitro fertilization and embryo transfer (IVF-ET), a significant proportion of women still do not become pregnant after long-term infertility, whether it is originally due to older age or other undetermined factors. In the present study, macrophage activating Chinese herbs (MACH) were evaluated for their effects on embryo qualities in women who were undergoing repeated IVF-ET because of long-term infertility. Thirty women, who had significantly low rates of developing good quality cleaved embryos and did not become pregnant after three or more cycles of Assisted Reproductive Technology (ART) procedure, were included in the study. Oral administration of MACH significantly increased the percentage of good quality early stage blastocysts (the number of grade 1 or grade 2 cleaved embryos/the number of retrieved oocytes) from 18.7 ± 16.2% to 36.1 ± 27.1% (1.9-fold increase, p < 0.01). The rate of good quality early stage blastocysts increased in all patients. In 19 patients who desired embryo transfer using late stage blastocysts, MACH significantly increased the percentage of late stage blastocysts from the initial value of 14.8 ± 11.2% to 21.1 ± 23.1% (1.4-fold increase, p < 0.05). The rate of embryonic progress into late stage blastocyst increased in 52.6% (10/19) of the patients. Furthermore, treatment with MACH significantly decreased the plasma follicle stimulating hormone (FSH) concentration on the day of oocyte retrieval from 14.4 ± 3.2 to 10.5 ± 2.4 mIU/ml (p < 0.05). No adverse events were observed with MACH supplementation, and there was no patient dropout. Administration of MACH resulted in improved embryo quality in the difficult cases. The present study demonstrates a new benefit of this herbal blend in women with refractory infertility of unknown etiology.

Oocyte quality improvement using a herbal medicine comprising 7 crude drugs

“…Our herbal medicine was based on MACH, which comprises Plantaginis semen, Carthami flos, Lonicerae flos, and Cucurbita moschata Duch. Each of the 4 crude drugs induces inferferon [17]. MACH modulates the macrophage function and is involved in the production of immunoglobulin E antibody [17].…”

“…Each of the 4 crude drugs induces inferferon [17]. MACH modulates the macrophage function and is involved in the production of immunoglobulin E antibody [17]. After MACH intake, blood pressure and skin surface blood flow decreased [22].…”

“…Several studies attempted to improve oocyte quality and ovum function by adjuncts to assisted reproductive technology (ART), such as dehydroepiandrosterone [13,14] or herbal medicine [15][16][17] administration, low reactive-level laser therapy (LLLT) [18], or acupuncture [19]. Reportedly [15,17,20,21], successful pregnancies were achieved after herbal medicine intake.…”

“…Reportedly [15,17,20,21], successful pregnancies were achieved after herbal medicine intake. Recently, it was reported [17,20,21] that women who repeatedly failed to conceive by ART became pregnant after macrophageactivating Chinese mixed herbs (MACH) intake.…”

“…Reportedly [15,17,20,21], successful pregnancies were achieved after herbal medicine intake. Recently, it was reported [17,20,21] that women who repeatedly failed to conceive by ART became pregnant after macrophageactivating Chinese mixed herbs (MACH) intake. MACH comprises Plantaginis semen, Cucurbita moschata Duch, Lonicerae flos, and Carthami flos; it is known to ameliorate the clinical phenomena associated with menopause [22].…”