Clinical evaluation of the CA530‐VET hematology analyzer for use in veterinary practice

Roleff, Sabine; Arndt, Gisela; Bottema, Bram; Junker, Lothar; Grabner, A; Kohn, Barbara

doi:10.1111/j.1939-165x.2007.tb00202.x

Cited by 22 publications

(28 citation statements)

References 10 publications

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“…10,16 In horses, cats, and dogs, GRAN are predominant in the blood (Table 1), therefore imprecision for this leukocyte subtype is low, and the Mythic 18 showed excellent agreement with both the Sysmex XT-2000iV and the manual WBC differentiation. 17 Agreement in canine and feline LYM counts in the Mythic 18 was not satisfactory. This finding has already been demonstrated for both species in the VetScan HMT, 6 and for canine samples in the CA530-Vet 17 and the Heska CBC (Becker M: 2007, A comparative study of seven in-house and two laboratory hematology instruments.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Mythic 18 hematology analyzer for use with canine, feline, and equine samples

et al. 2011

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Abstract. The Mythic 18 is a fully automated hematology bench-top analyzer using impedance technology for a complete blood cell count (CBC) and a 3-part white blood cell count (WBC) differential. The purpose of the current study was to evaluate the Mythic for assessment of agreement, precision, linearity, carry-over, stability, and usability under practice conditions. Ethylenediamine tetra-acetic acid-blood samples from 122 dogs, 140 cats, and 123 horses were analyzed with the Mythic and reference methods (Sysmex XT-2000iV, manual hematocrit, and microscopic WBC differentiation). Pearson's coefficient of correlation, Passing-Bablok regression analysis, and Bland-Altman difference plots were performed to determine agreement. For precision, standard deviation and coefficients of variation were calculated. Linearity was determined according to Emancipator-Kroll. Red blood cell parameters showed excellent correlation and small biases, except for red cell distribution width and mean corpuscular hemoglobin concentration. Total WBC correlated excellently in canine and equine and very well in feline samples. In 23 feline specimens with platelet aggregates, the Mythic overestimated WBC. In all 3 species, absolute granulocyte counts correlated excellently. Equine lymphocyte counts showed good correlation whereas canine and feline lymphocyte counts correlated poorly. Feline platelets showed good correlation with a negative bias. The instrument showed good to excellent precision. The whole 3-part differential was found to be accurate in horses. In dogs and cats, absolute granulocyte counts were reliable. As with all impedance-based hematological instruments, evaluation of a blood smear is absolutely indicated to check for the presence of platelet aggregates, to verify WBC differentiation, and to identify possible abnormalities.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Mythic 18 hematology analyzer for use with canine, feline, and equine samples

et al. 2011

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“…Os achados obtidos neste estudo foram semelhantes aos encontrados por Roleff et al (2007), que utilizaram a mesma metodologia em amostras das espécies felina e equina e aos de Vieira et al (2007) e que verificaram uma boa correlação entre a determinação do VG por impedância e método de Strumia em cães. Dewhurst et al (2003), observaram excelente correlacao do VG determinado por impedância utilizando dois aparelhos comerciais em amostras de cães e gatos.…”

Section: Discussionunclassified

“…Os contadores hematológicos eletrônicos, inicialmente desenvolvidos para humanos, têm sido adaptados para animais que apresentam células sangüíneas com maior variabilidade morfométrica e morfológica, além de maior tendência para aglutinar do que o sangue humano (Roleff et al, 2007). A maioria desses equipamentos veterinários foi recentemente introduzida no mercado e necessitam de validação para diferentes espécies.…”

Section: Introductionunclassified

“…A maioria desses equipamentos veterinários foi recentemente introduzida no mercado e necessitam de validação para diferentes espécies. Neste sentido, são escassos os estudos que avaliaram a correlação entre os valores de VG obtidos por impedância e método de Strumia (Strumia et al, 1954) para as espécies felina, equina e bovina (Dawson, et al, 2000;Dewhurst et al, 2003;Roleff et al, 2007;Weingand et al, 1992). Recentemente, Vieira et al (2007) verificaram que existe uma boa correlação entre os métodos utilizando amostras de cães.…”

Section: Introductionunclassified

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Uso Da Impedância Na Determinação Do Volume Globular Das Espécies Felina, Equina E Bovina

Vieira¹,

Viol²,

Barbosa³

et al. 2008

AVS

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Endereço para correspondência: Paulo Cesar Cialini -ciarlini@fmva.unesp.br R RE ES SU UM MO OObjetivou-se determinar a correlação entre os valores de volume globular (VG) das espécies felina, equina e bovina com dois diferentes métodos: por impedância e pelo método de Strumia. Para tal, amostras de sangue de 70 gatos, 80 cavalos e 77 bois foram analisadas. Os valores de VG, em ambos os métodos, foram obtidos num período compreendido entre 20 -120 minutos após a colheita. Os valores de VG obtidos pelos métodos de Strumia e impedância apresentaram correlação (r = 0,98 gatos, r = 0,98 cavalos e r = 0,96 bois) extremamente significante (p < 0,001) para as três espécies. Concluiu-se que o valor do VG determinado por impedância revelou-se confiável e é recomendado para o uso clínico nas espécies felina, equina e bovina. Palavras-chave: hematócrito, Strumia, volume globular, impedância A AB BS ST TR RA AC CT TThe aim of this study was to determinate the correlation of packed cell volume (PCV) between cats, horses and cows with two different methods: by impedance and by the Strumia method. For this purpose, blood samples from 70 cats, 80 horses and 77 cows were analyzed. The PCV data, in both methods, were determined at 20 -120 minutes after blood collection. The PCV data obtained by Strumia and impedance methods (r = 0.98 cats, r = 0.98 horses and r = 0.96 cows) were extremely significant (p < 0.0001) for the three species. In conclusion, the PCV data determined by impedance was reliable and it is recommended for clinical use in feline, equine and bovine species.

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“…It is available with multiple preprogrammed settings for the commonest domestic species. Although comparative studies have been conducted between automated hematology systems and manual techniques using samples of most of the domestic species Science Publications AJAVS (Hennessy et al, 1998;Bienzle et al, 2000;Dawson et al, 2000;Papasouliotis et al, 2006;Roleff et al, 2007), such a comparison has not been made for ovine blood except for a comparison of blood samples from lambs collected into two types of vacutainer tubes, one containing EDTA and the other heparin (Vatn et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of the Ovine Haemogram: Cell-Dyn 3500 Versus Manual Methods

Athanasiou¹

2013

American Journal of Animal and Veterinary Sciences

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Manual hematology techniques traditionally applied in farm animal medicine are time consuming and laborintensive, especially when large numbers of samples have to be processed. As a result, several automated hematology instruments have been developed for use in these species. An automated hematology analyzer (Abbott Cell-Dyne 3500 system) was used in performing a complete blood cell count and differential counts of white blood cells in sheep blood samples. The system was compared with basic manual hematologic techniques. A linear regression was used to assess correlation between the two methods. Correlation coefficients (R2) were good for the hematocrit, the total white blood cell count, the neutrophils, the lymphocytes and the platelet count, while a poor correlation existed in monocytes, eosinophils and basophils. The automated and the manual technique were also compared in terms of sensitivity using the Sensitivity Ratio (SR). The automated analyzer was slightly more sensitive than the manual technique for all parameters tested except for monocytes, eosinophils and basophils were the difference was greater and the automated analyzer was 1.5, 2.5 and 2 times more reliable, respectively. The method bias was also calculated. It seems that the overall performance of the automated analyzer justifies its utilization in sheep blood analysis, although as for any analyzer used in any species, a stained blood film evaluation remains an indispensable technique to confirm the results being reported by the automated analyzer and provide additional information for the ovine haemogram.

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Clinical evaluation of the CA530‐VET hematology analyzer for use in veterinary practice

Abstract: The CA530-VET can be considered useful for routine canine, feline, and equine blood cell analyses. It should not be considered accurate, however, for PLT counts, feline total WBC counts in the subnormal and normal range, and leukocyte differentials, except for granulocytes.

Cited by 22 publications

References 10 publications

Evaluation of the Mythic 18 hematology analyzer for use with canine, feline, and equine samples

Evaluation of the Mythic 18 hematology analyzer for use with canine, feline, and equine samples

Uso Da Impedância Na Determinação Do Volume Globular Das Espécies Felina, Equina E Bovina

A Comparative Study of the Ovine Haemogram: Cell-Dyn 3500 Versus Manual Methods

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