Clinical Outcome After Intra‐Articular Administration of Bone Marrow Derived Mesenchymal Stem Cells in 33 Horses With Stifle Injury

Abstract

Objective To report outcome of horses with femorotibial lesions (meniscal, cartilage or ligamentous) treated with surgery and intra‐articular administration of autologous bone marrow derived mesenchymal stem cells (BMSCs). Study Design Prospective case series. Animals Horses (n = 33). Methods Inclusion criteria included horses that had lameness localized to the stifle by diagnostic anesthesia, exploratory stifle arthroscopy and subsequent intra‐articular administration of autologous BMSCs. Case details and follow‐up were gathered from medical records, owner, trainer or veterinarian. Outcome was defined as returned to previous level of work, returned to work, or failed to return to work. Results Follow‐up (mean, 24 months) was obtained; 43% of horses returned to previous level of work, 33% returned to work, and 24% failed to return to work. In horses with meniscal damage (n = 24) a higher percentage in the current study (75%) returned to some level of work compared to those in previous reports (60–63%) that were treated with arthroscopy alone, which resulted in a statistically significant difference between studies (P = .038). Joint flare post injection was reported in 3 horses (9.0%); however, no long‐term effects were noted. Conclusions Intra‐articular administration of BMSC postoperatively for stifle lesions appeared to be safe, with morbidity being similar to that of other biologic agents. Improvement in ability to return to work may be realized with BMSC treatment compared to surgery alone in horses with stifle injury.

Osteoarthritis in horses - Part 2: a review of the intra-articular use of corticosteroids as a method of treatment

“…Nevertheless, several medications are used intra-articularly, such as COs, hyaluronic acid (HA)/sodium hyaluronate (SH)/hyaluronate (HA), polysulfated glycosaminoglycans, sodium pentosan polysulfate, sodium chondroitin sulfate, somatostatin, N-acetyl- D-glucosamine, radiation synovectomy, dextrose prolotherapy, keratan sulfate orgotein, saline for washout, silicone, mucopolysaccharide polysulfuric acid ester, chloroquine, lactic acid solution, amikacin, gentamicin morphine, autologous serum, platelet-rich plasma, mesenchymal stem cells derived from bone marrow or adipose stromal vascular fraction, gene therapy using IL-1 antagonists, and ethanol. In addition, studies have used a topical liposomal formulation that contains diclofenac, lyophilized products from green-lipped mussel, extracorporeal shockwave, acupuncture, magnetic field, transcutaneous electrical nerve stimulation, therapeutic ultrasound, laser therapy, manual therapy, aquatic physical therapy and nutraceuticals food suplement (Uthman et al 2003;Caron 2005;Revenaugh 2005;Carmona et al 2007;Frisbie et al 2009;Goodrich and Nixon 2006;Santos et al 2009;Lindegaard et al 2010;Cayzer et al 2011;Kawcak et al 2011;Baccarin et al, 2012;McIIwraith et al 2011McIIwraith et al , 2012aCarmalt et al 2012;Lamas et al 2012;Vanderweerd et al 2012;Frisbie et al 2013;King et al 2013;Neuman et al 2013;Ferris et al 2014;Koenig et al 2014, de Grawn et al 2016. The use of controlled physical activity, as a complementary treatment, possibly improves the comfort level of the horse and decreases the inflammatory process (Kerbyson et al 2013).…”

Into the eyes of bone marrow-derived mesenchymal stem cells therapy for myocardial infarction and other diseases

“…Besides, BM-MSCs are relatively primitive cells, whose immunogenicity is weak and can inhibit the mixed lymphocyte reaction. However, previous studies also reported the clinical application of BM-MSCs is not matched with donor, host immune rejection or graft versus host reaction (50)(51)(52). Moreover, allogeneic bone marrow transplantation of mesenchymal stem cells in severe idiopathic aplastic anemia patients have also been shown to improve the effect of bone marrow stromal function (53,54).…”

Advances in osteosynthesis in the horse

“…Die minimal-invasive Frakturfixation wird bei Kleintieren schon seit einigen Jahren praktiziert (Palmer 1999, Pozzi et al 2013 (Jang et al 2008, Fayaz et al 2011, Rolim Filho et al 2013, Ferris et al 2014 …”