Clinical significance of EphA2 expression in squamous-cell carcinoma of the head and neck

Abstract

These findings suggested that EphA2 may contribute to SCCHN progression and represent a novel prognostic indicator for patients with SCCHN.

Downregulation of EphA2 expression suppresses the growth and metastasis in squamous-cell carcinoma of the head and neck in vitro and in vivo

“…Recent studies in our laboratory have demonstrated that EphA2 overexpression was signiWcantly associated with SCCHN aggressive behavior and poor prognosis from the analysis of clinical tissue specimens (Liu et al 2011). Similar results in clinical tissue level have been obtained by other investigators (Wu et al 2011;Shao et al 2008;Rivera et al 2008).…”

“…In our previous publication (Liu et al 2011), close associations between the expression of EphA2 protein and several clinicopathological parameters, such as tumor site, T classiWcation, clinical stage, recurrence and lymph node metastasis, have been clearly conWrmed in a relative large SCCHN tissue specimens. Moreover, overexpression of EphA2 predicts a poor prognosis in patients with SCCHN.…”

“…The details of RT-PCR have been previously described (Liu et al 2011). BrieXy, total RNA of cells was isolated and transcribed to cDNA, and the PCR cycling conditions was as follows: 95°C for 5 min; then 32 cycles at 95°C for 30 s, 58°C for 30 s, and 72°C for 30 s; and an extension at 72°C for 5 min.…”

EphA2 silencing in nasopharyngeal carcinoma leads to decreased proliferation, invasion and increased sensitization to paclitaxel

“…Our previous investigations have clearly demonstrated EphA2 overexpression in human laryngeal and hypopharyngeal squamous cell carcinoma, and EphA2 regulation of the growth and cervical lymph node metastasis of human laryngeal and hypopharyngeal squamous cell carcinoma in vitro and in vivo (20,22). Although NPC is also classified as a subtype of head and neck squamous cell carcinoma, it possesses unique and distinct epidemiology, clinical characteristics, etiology and histopathology (2).…”

“…The western blot analyses were performed as described in previous studies (19,20). In brief, total protein (50 µg/sample) was extracted and separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto PVDF membranes (Millipore, Bedford, MA, USA).…”