Clinical significance of fluoroscopic patterns specific for the mitotic spindle in patients with reumatic diseases

Abstract

Objective: we proposed to determine the clinical significance of anti-NuMA and anti-HsEg5 antibodies in a group of patients affected with rheumatic diseases. Materials and methods: indirect immunofluorescence on HEp-2000 cells at serum dilution of 1:40 was used to examin 26 sera which had previously showed a “mitotic spindle” fluoroscopic pattern type during laboratory routine. Results: 21 sera (80,7%) were identified with NuMA and 5 (19,3%) with HsEg5 patterns alone or associated with other ANA patterns. However only patients with isolated positiveness and that is 15 with NuMA and 4 with HsEg5 stainings were included in this study. Of the NuMA positive patients 5 were affected with arthropathies associated to different forms of thyroiditis, 2 with seronegative arthritis, 2 with antiphospholipid syndrome, 1 with systemic lupus erythematosus (SLE), 1 with rheumatoid arthritis, 1 with sicca syndrome, 1 with undifferentiated connective tissue disease, 1 with Mycoplasma pneumaniae infection and 1 with retinal thrombosis. Of the HsEg5 positive patients 3 were affected with SLE and 1 with seronegative arthritis. Conclusions: NuMA does not prevail in any defined rheumatic disease, while HsEg5 staining were more frequent (75%) in patients affected with SLE all of whom showing high antibody titres

Predictive value of antinuclear antibodies in autoimmune diseases classified by clinical criteria: Analytical study in a specialized health institute, one year follow-up

“…In this study 2% of patients had NuMA, and they were associated with primary AS, one of them with optic neuritis and a possible Devic syndrome. The prevalence of these antibodies and their clinical significance has been previously reported in the literature [43] , [44] .…”

Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA patterns (ICAP) perspective

“…The clinical relevance of the five mitotic patterns is summarised in table 3,102–122 with more detailed information in online supplementary table S3. As for the cytoplasmic patterns, clinical associations for the mitotic patterns are primarily based on antigen-specific immunoassays and not on the HEp-2 IIFA pattern as such.…”

Anti‐Mitotic Spindle Apparatus Antoantibodies: Prevalence and Disease Association in Chinese Population

“…Anti-mitotic spindle apparatus (anti-MSA) antibodies, described by McCarty et al in 1981 (5), are infrequently detected in ANA routine IIF (indirect immunofluorescence) screening of human pathological sera (6). MSA, a unique microtubule or protein structure involved in mitosis and postmitotic nuclear reorganization (7,8), is composed of two main antigens, the nuclear mitotic apparatus protein (NuMA1) and spindle kinesin-like protein HsEg5 (NUMA2) (9)(10)(11).…”