Clinical significance of HSP27 expression in colorectal cancer

Abstract

Abstract. The heat shock protein 27-kda (HSP27) has been found overexpressed in several types of human cancer and is associated with treatment resistance and poor prognosis. Recent proteomic studies demonstrate that HSP27 is significantly overexpressed in colorectal cancer (crc). However, the relationship between HSP27 expression and patient prognosis remains nascent. in the present study, we aimed to investigate the expression of HSP27 and its correlation with clinicopathological parameters in crc patients. dysregulated expression of HSP27 was observed in neoplastic lesions, and appears to be involved in disease progression. immunohistochemical analysis showed that detectable HSP27 expression was found in 145/182 (79.7%) CRC cases. There was a significant correlation between HSP27 expression and TnM stage (P=0.003). Patients with low HSP27 expression had better survival than those with high HSP27 expression. additionally, multivariate analysis indicated that HSP27 expression is an independent prognostic marker for crc. These results suggest that elevated expression of HSP27 protein is a frequent event during the progression of crc. HSP27 might be used as a valuable prognostic marker for patients with crc. Introductioncolorectal cancer (crc) is one of the leading causes of malignancy-related deaths worldwide (1). The prognosis of crc patients remains poor, with a 5-year survival rate of ~45% reported in most studies, despite significant improvements in early diagnosis, surgery, chemotherapy and radiotherapy (2). although TnM (tumor-node-metastasis) stage has been accepted as the most significant and independent prognostic factor, there are still many patients at the same stage who have different clinical outcomes (3). Therefore, the investigation and application of molecular markers responsible for the development and progression of crc are of utmost importance.Heat shock proteins (HSPs) are detectable in virtually all organisms, from prokaryotes to mammals, and play a fundamental role in the maintenance of cellular homeostasis (4). under physiological conditions, HSPs are constitutively expressed at a low level and function as molecular chaperones that fulfill important roles for protein folding, cellular signaling and protein degradation (5). HSPs have a stimulated synthesis in response to a variety of stressful stimuli (e.g., heat, heavy metal, infection or inflammation) and promote the refolding of damaged proteins (6). HSPs are classified by their molecular weight; the main HSP families comprise HSP110, HSP90, HSP70, HSP60 and the small HSPs (7).HSP27, a small HSP, is ubiquitously expressed at low levels in normal cells (8). in contrast, its aberrant expression has been reported in a variety of human cancers, including breast (9), ovarian (10), gastric (11), prostate (12), endometrial (13), liver (14), bladder (15) and leukemia (16). Furthermore, elevated HSP27 levels in breast, ovarian, gastric, and prostate cancer is associated with aggressive growth and resistance to chemotherapy or radiotherapy, and hen...

Stromal Expression of Heat-Shock Protein 27 Is Associated with Worse Clinical Outcome in Patients with Colorectal Cancer Lung Metastases

“…On the one hand, this small heat-shock protein has been examined in CRC tumor cells themselves [ 34 – 36 ]. An increased expression of Hsp27 in tumor cells of primary CRC was described as negative prognosticator for survival [ 37 , 38 ]. On the other hand, Hsp27 expressing fibroblasts were examined in the context of wound healing and fibrosis.…”

Identification of biomarkers for lymph node metastasis in early-stage cervical cancer by tissue-based proteomics

“…Its functions can be triggered by different types of stress such as inflammation, oxidative stress, and malignant transformation (Ciocca and Calderwood, 2005). HspB1 is overexpressed in several types of malignancies (Huang et al , 2010; Yu et al , 2010) and has been correlated with poor prognosis (Yu et al , 2010). Moreover, it has been reported that upregulation of HspB1 is associated with tumour metastasis (Nagaraja et al , 2012).…”

Expression of heat shook protein 27 and proliferating cell nuclear antigen in human retinoblastoma

“…Heat shock protein 27 expression reflects the malignant potentials and prognoses of tumors [21–23]. Heat shock protein 27 overexpression is associated with tumor infiltration in breast cancer, such that HSP27 antibody treatment can increase the survival rate of patients with breast cancer [12]. Therefore, further investigation into HSP27 expression in patients with tumors and the regulatory mechanism of HSP27 expression is of great clinical significance in the treatment of tumors.…”