Clinical significance of microRNA-24 expression in esophageal squamous cell carcinoma

Abstract

microRNA-24 has been reported to participate in tumorgenesis and progression by several signaling pathways in various tumors. However, its potential as a serum diagnostic factor and predictive biomarker for esophageal squamous cell carcinoma (ESCC) has not been studied. in the present study, serum samples were collected from 105 pathologically proven ESCC patients and 30 healthy volunteers. All patients were treated with concurrent chemotherapy and radiotherapy. Real-time polymerase chain reaction was carried out to measure the serum miR-24 expression level in all patients and volunteers. The data were compared among radio-sensitive group (CR+PR, 62 patients), radio-resistant group (SD+PD, 43 patients) and healthy volunteers to elucidate the diagnostic value of serum miR-24 testing for ESCC and the predictive value of miR-24 expression of treatment response. in the result, of the 105 ESCC patients enrolled in the study, 62 patients achieved partial or complete response. The serum miR-24 level in ESCC patients is 4.82 times as high as that in healthy subjects(P<0.01), indicating that serum miR-24 expression could be an excellent diagnostic factor. The mean miR-24 serum levels differ by 2.05 folds between radiosensitive group and radioresistant group, indicating that it may serve as a biomarker for predicting the response of ESCC patient to CRT. Furthermore, the responsiveness of therapy is significantly correlated with Cyfra21-1(P<0.05), serum miR-24 level (P<0.05) and the myelosuppression (P<0.01). in the prsent study, we come to the conclusion that serum miR-24 has the potential to serve as a noninvasive biomarker for both ESCC diagnosis and predicting treatment responses to concurrent chemo-radiation therapy. ESCC patients with lower Cyfra21-1, higher miR-24, and severer myelosupression were much more sensitive to CRT. Key words: miR-24 expression, esophageal squamous cell carcinoma, chemo-radiation therapy, radiosensitivityEsophageal cancer (EC) is the sixth most common cancer and the fifth leading cause of cancer death in men worldwide, there was an estimated 482,300 new EC cases and 406,800 EC-caused deaths in 2008 worldwide [1]. Despite improved treatments in recent years, curative surgical resection, which is initially recommend for early stage cases, feasible for only 30-40% of patients [2]. While the outcome of surgery for patients with such an aggressive tumor has been unsatisfactory, with a 5-year survival rate less than 20% [3].Prognostic assessment is critical for making better therapeutic choices for ESCC patients, and the tumor-nodemetastasis (TNM) staging system is the key prognostic determinant. However, the variations in clinical responses to CRT are most evident for esophageal squamous cell carcinoma (ESCC), and the survival rates between responders and non-responders are quite different even in the same clinical stage [4,5]. Rapidly increasing findings on cancer biology provide prognostic information that complements and, in some cases, is more relevant than anatomical extent [6]. The discove...

MicroRNA-1285 inhibits malignant biological behaviors of human pancreatic cancer cells by negative regulation of YAP1

“…The connections between microRNAs and chemoresistance have been the subject of much investigation in recent years. MicroRNAs may regulate chemosensitivity through regulating drug excretion, cell survival signal, cell cycle, cell apoptosis, EMT, cancer stem cell property, etc [11,12].…”

Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma

“…Clinical data of enrolled patients including gender, age, tumor locations, status of lymph node and distant metastases, the maximum diameter of tumor and tumor differentiation were recorded. Entry criteria and treatment regimen for the present study have been described in our published study (31). Immediately after collection, the serum samples were snap-frozen in liquid nitrogen and then stored at -80˚C for RNA extraction for quantitative RT-PCR (qRT-PCR).…”

“…miR-7 was detected by qRT-PCR using the mirVana™ qRT-PCR primer set and the mirVana™ qRT-PCR miRNA detection kit (Applied Biosystems, San Diego, CA, uSA) according to the manufacturer's instructions, which were previously described (31).…”

“…Real-time polymerase chain reaction (RT-PCR) was carried out on the ABI 7900 Real-Time PCR System (Applied Biosystems). Relative microRNA expression was calculated with the 2 -ΔΔCt method (31,33), where ΔΔCt = (Ct gene of interest -Ct normalized gene) of (CR+PR) -(Ct gene of interest -Ct normalized gene) of (SD+PD).…”

A abordagem de <i>high-content screening</i> para identificação de miRs com potencial terapêutico no câncer de cabeça e pescoço

“…neoplásicos oriundos de TSCC e ESCC, em comparação com tecidos saudáveis(DONG et al, 2015;ZHAO et al, 2016). Por outro lado, em linha com nossos achados, estudos envolvendo análises de expressão gênica e ensaios funcionais encontraram a subexpressão do miR-24 em tecidos neoplásicos oriundos de LSCC, em comparação com tecidos saudáveis, bem como sua atividade supressora tumoral em células oriundas de linhagens derivadas de LSCC(GUO et al, 2012;XU et al, 2015).…”