Clinical utility of red blood cell carbonic anhydrase I and zinc concentrations in patients with thyroid diseases

Yoshida, Katsumi; Kiso, Yoshinori; Watanabe, Takao; Kaise, Kazuro; Kaise, Nobuko; Fukazawa, Hiroshi; Mori, Keiichiro; Abe, Keishi; Yoshinaga, Kaoru

doi:10.1016/0026-0495(91)90128-j

Cited by 15 publications

(13 citation statements)

References 13 publications

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“…Following an antithyroid therapy, normalization of these parameters, lagged behind that of plasma thyroid hormone levels by about 2 months (5). In another study, RBC Zn concentration in patients with hyperthyroidism before and after treatment with antithyroid drugs was reported (4). Therein, the normalization of RBC Zn concentration lagged about 2 months behind the normalization of plasma T 4 and T 3 levels after antithyroid drug treatment.…”

Section: Resultsmentioning

confidence: 95%

“…It has been reported that activity levels of CA isoenzymes in human erythrocytes vary considerably under certain pathological and physiological conditions. CA activity has been associated with metabolic diseases such as diabetes mellitus and hypertension and thyroid diseases (3,4). Since most of the Zn in RBC is present as the metal of CA (5), and since thyroid hormones inhibit the synthesis of RBC CAI isoenzyme, the concentration of RBC CAI, as well as Zn, is decreased in hyperthyroidism (4,6,7).…”

Section: Introductionmentioning

confidence: 99%

“…CA activity has been associated with metabolic diseases such as diabetes mellitus and hypertension and thyroid diseases (3,4). Since most of the Zn in RBC is present as the metal of CA (5), and since thyroid hormones inhibit the synthesis of RBC CAI isoenzyme, the concentration of RBC CAI, as well as Zn, is decreased in hyperthyroidism (4,6,7). It has been reported that clinical utility of determining RBC CAI and Zn concentration was found in patients with various forms of thyroid disease (4,8).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of Red Blood Cell Carbonic Anhydrase, Glucose 6-Phosphate Dehydrogenase, Hexokinase Enzyme Activities, and Zinc Concentration in Patients With Hyperthyroid Diseases

et al. 2002

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We have recently reported on the activities of glucose-6-phosphate dehydrogenase (G6PD), hexokinase (HK) and carbonic anhydrase I (CA-I) and II (CA-II) isoenzymes obtained from erythrocytes of healthy subjects and untreated patients with hyperthyroid diseases. Also, erythrocyte zinc concentrations were measured. Red blood cell (RBC) zinc (Zn) concentration was measured by atomic absorption spectrophotometry. Activities of carbonic anhydrase II and I isoenzymes were determined with CO2-hydratase activity method by using selective inactivation with bromopyruvate. G6PD and HK enzyme activities were measured spectrophotometrically via absorbance change (at 340 nm) in NADPH formed as a result of the reactions catalysed by these enzymes. In statistical analysis of all these parameters, activity of CA-I was 4388 +/- 207 (EU/gHb) and 2881 +/- 869 (EU/gHb) in healthy and untreated hyperthyroid subjects, respectively. The activity values for CA-II were 5391 +/- 257 (EU/gHb) and 4688 +/- 12.6 (EU/gHb) in healthy and untreated hyperthyroid subjects. Glucose 6-phosphate dehydrogenase (G6PD) activity was 10.19 +/- 1.87 (EU/gHb) in healthy group and 4.92 +/- 2.49 (EU/gHb) in patient group. While hexokinase enzyme activity was 1.575 +/- 0.898 in healthy subjects, it was 0.651 +/- 0.418 (EU/gHb) in the patient group. While erythrocyte zinc concentration in the healthy subjects was 49.32 +/- 23.5 (mg/gHb), this concentration for patients with uncontrolled hyperthyroid diseases was significantly decreased to 29.62 +/- 4.26 (mg/gHb). As a conclusion, CA-I isoenzyme, G6PD, hexokinase activities and erythrocyte zinc concentration had decreased in untreated patients carrying hyperthyroid diseases as compared to those of the healthy subjects.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of Red Blood Cell Carbonic Anhydrase, Glucose 6-Phosphate Dehydrogenase, Hexokinase Enzyme Activities, and Zinc Concentration in Patients With Hyperthyroid Diseases

et al. 2002

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confidence: 99%

“…We have previously reported [4][5][6] in patients with hyperthyroidism that the RBC Zn concentration reflects a patient's mean thyroid hormone level over the preceding months as glycosilated hemoglobin does in diabetic patients [7]. Measurement of the RBC Zn concentration is therefore useful in differentiating hyperthyroid Graves' disease from transient thyrotoxicosis [5,6]. But our previously reported method for measuring the RBC Zn concentration is troublesome [4]: packed RBC is block digested in the presence of acid (0.8 ml of concentrated nitric acid and 0.2 ml of concentrated perchloric acid in a micro Kjeldahl tube) and measured by atomic absorption spectrophotometry.…”

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confidence: 99%

Measurement of Red Blood Cell Zinc Concentration with Zn-Test Kit: Discrimination between Hyperthyroid Graves' Disease and Transient Thyrotoxicosis.

et al. 1998

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Abstract.We have previously reported in patients with hyperthyroidism that the red blood cell (RBC) zinc (Zn) concentration reflects the mean thyroid hormone concentration over the preceding months. In the present study, the concentration of RBC Zn was measured by a simple and easy method with a Zntest Wako kit.Within-run and between-run precision were 1.4% and 1.3%, respectively. The relationship between RBC concentration and dilution was linear. The average recovery was 103%. A good correlation (r=0.97) was obtained between this method and atomic absorption spectrophotometry.

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Calcium, magnesium, and zinc status in experimental hyperthyroidism

Şimşek

Andican

Unal

et al. 1997

Biol Trace Elem Res

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In this study, experimental hyperthyroidism was established and used to investigate possible alterations in the calcium (Ca), magnesium (Mg), and zinc (Zn) homeostasis by assessing their concentrations in plasma and erythrocytes. In the L-thyroxine-induced hyperthyroidism condition, the experimental animals show a significant decrease in erythrocyte Ca, Mg, and Zn concentrations, and a significant decrease in plasma Mg concentration. Significant positive correlations were found for Mg and Zn both in plasma and in erythrocytes. The results suggest that the homeostasis of Ca, Mg, and Zn is altered during experimental hyperthyroidism.

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Clinical utility of red blood cell carbonic anhydrase I and zinc concentrations in patients with thyroid diseases

Cited by 15 publications

References 13 publications

Investigation of Red Blood Cell Carbonic Anhydrase, Glucose 6-Phosphate Dehydrogenase, Hexokinase Enzyme Activities, and Zinc Concentration in Patients With Hyperthyroid Diseases

Investigation of Red Blood Cell Carbonic Anhydrase, Glucose 6-Phosphate Dehydrogenase, Hexokinase Enzyme Activities, and Zinc Concentration in Patients With Hyperthyroid Diseases

Measurement of Red Blood Cell Zinc Concentration with Zn-Test Kit: Discrimination between Hyperthyroid Graves' Disease and Transient Thyrotoxicosis.

Calcium, magnesium, and zinc status in experimental hyperthyroidism

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