Clinical value of serum miR‐320‐3p expression in predicting the prognosis of sepsis‐induced acute kidney injury

Abstract

Background For investigating the expression of miR‐320‐3p in children with sepsis‐induced acute kidney injury (AKI) and its prognostic value. Methods A total of 142 patients were grouped into a survival group (n = 95) and death group (n = 47), which was based on their 28‐day survival. Serum degrees of miR‐320‐3p, neutrophil gelatinase‐associated lipid carrier protein (NGAL) and kidney injury molecule‐1 (KIM‐1) were detected. The Acute Physiology and Chronic Health scoring system Ⅱ (APACHE Ⅱ) marks were recorded. Target gene forecast and functional enrichment discussion of miR‐320‐3p were performed, and a protein–protein interaction (PPI) network diagram was plotted by applying bioinformatics methods. Multivariate logistic regression, ROC curve and Pearson correlation analysis were applied. Results The death group showed greatly higher serum levels of miR‐320‐3p, KIM‐1 and APACHE Ⅱ scores than the survival group (p < 0.01). Multivariate logistic regression analysis showed that levels of miR‐320‐3p, NGAL, KIM‐1 and APACHE Ⅱ scores were independent risk elements for death in sepsis children with AKI (p < 0.01). According to ROC curve analysis, the region under the curve (0.963, 95% CI: 0.908–0.996) of miR‐320‐3p, NGAL, KIM‐1 levels and APACHE Ⅱ scores combined to forecast the death of kids suffering from sepsis and AKI were the biggest. According to correlation analysis, the expression degree of serum miR‐320‐3p in the death group was positively correlated with NGAL, KIM‐1 and APACHE Ⅱ scores (all p < 0.01). Conclusions The expression level of serum miR‐320‐3p in children with sepsis‐induced AKI was significantly increased, and the combination of NGAL, KIM‐1 and APACHE Ⅱ scores has good value for prognosis prediction in children.

Identification of hub genes associated with acute kidney injury induced by renal ischemia–reperfusion injury in mice

“…Bioinformatics analyses have enabled us to understand the molecular mechanisms of AKI occurrence and development and have been used to explore potential targets for AKI diagnosis and treatment. Previous microarray studies have often focused on the role of a single molecule and are largely based on a sepsis-induced AKI model ( Tang et al, 2021 ; Ji et al, 2022 ). However, the vast majority of AKI in clinical work is associated with ischemic–reperfusion injury, and there have been some studies on the gene expression of IRI-AKI ( Wu et al, 2016 ; Wu et al, 2019 ).…”

MicroRNAs in septic acute kidney injury

“…Serum levels of miR-21-3p, in combination with Scr, Cys-C and kidney injury molecule 1 (KIM-1), were significantly increased in children with sepsis and could predict AKI onset with a sensitivity of 97% and specificity of 91.4% [ 47 ]. Another clinical study revealed that serum miR-320-3p levels were greatly enhanced in children with sepsis-induced AKI, and combining NGAL, KIM-1 and acute physiology and chronic health evaluation (APACHE) II scores could be effective for predicting prognosis in children [ 24 ]. Serum and urinary miR-452 levels increased early after LPS or cecal ligation puncture (CLP) in septic mice, occurring earlier than detectable renal dysfunction or tissue damage.…”

“…Clinical trials and animal experiments have demonstrated that modulating miRNA expression is beneficial in several diseases [ 20–23 ]. Some miRNAs are increased in the urine and plasma of patients with septic AKI, suggesting that they might be valuable diagnostic biomarkers [ 24–29 ]. Increasing evidence indicates that miRNAs could be therapeutic targets against septic AKI [ 30–35 ].…”