Clonal Distribution and Virulence of<i>Campylobacter jejuni</i>Isolates in Blood

Feodoroff, Benjamin; Haan, C. P. A. de; Ellström, Patrik; Sarna, Seppo; Hänninen, Marja‐Liisa; Rautelin, Hilpi

doi:10.3201/eid1910.121537

Cited by 16 publications

(34 citation statements)

References 12 publications

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“…All except one of the 73 C. jejuni bacteraemia isolates had been earlier genotyped by MLST 17 . Among the isolates, the ST-677 CC was the most prevalent (35 isolates, 48%) followed by the ST-45 CC (12 isolates, 16%) and ST-21 CC (10 isolates, 14%).…”

Section: Methodsmentioning

confidence: 99%

“…We recently characterized these particular C. jejuni blood culture isolates with respect to their clonal distribution, by using multilocus sequence typing (MLST), and to their serum resistance. We found that nearly half of the isolates belonged to the otherwise uncommon sequence type (ST) 677 clonal complex (CC) (ST-677 CC) 17 . Isolates belonging to this particular CC were more resistant to NHS than the other isolates, but otherwise serum resistance was not a prerequisite for bacteraemic infection 17 .…”

Section: Introductionmentioning

confidence: 99%

“…We found that nearly half of the isolates belonged to the otherwise uncommon sequence type (ST) 677 clonal complex (CC) (ST-677 CC) 17 . Isolates belonging to this particular CC were more resistant to NHS than the other isolates, but otherwise serum resistance was not a prerequisite for bacteraemic infection 17 . In the present study, we further examined bacterial characteristics that might be important for the ability of C. jejuni to enter the bloodstream, and used PCR to screen for LOS locus class and for the presence of the putative virulence genes ceuE , ciaB , fucP , and virB11 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lipooligosaccharide locus class of Campylobacter jejuni: sialylation is not needed for invasive infection

Ellström

Feodoroff

Hänninen

et al. 2014

Clinical Microbiology and Infection

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Campylobacter jejuni is a highly diverse enteropathogen that is commonly detected worldwide. It can sometimes cause bacteraemia, but the bacterial characteristics facilitating bloodstream infection are not known. A total of 73 C. jejuni isolates, consecutively collected from blood-borne infections during a 10-year period all over Finland and for which detailed clinical information of the patients were available, were included. We screened the isolates by PCR for the lipooligosaccharide (LOS) locus class and for the presence of the putative virulence genes ceuE, ciaB, fucP, and virB11. The isolates were also tested for γ-glutamyl transpeptidase production. The results were analysed with respect to the clinical characteristics of the patients, and the multilocus sequence types (MLSTs) and serum resistance of the isolates. LOS locus classes A, B, and C, which carry genes for sialylation of LOS, were detected in only 23% of the isolates. These isolates were not more resistant to human serum than those with the genes of non-sialylated LOS locus classes, but were significantly more prevalent among patients with underlying diseases (p 0.02). The fucose permease gene fucP was quite uncommon, but was associated with the isolates with the potential to sialylate LOS (p <0.0001). LOS locus classes and some of the putative virulence factors were associated with MLST clonal complexes. Although some of the bacterial characteristics studied here have been suggested to be important for the invasiveness of C. jejuni, they did not explain why the clinical isolates in the present study were able to cause bacteraemia.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipooligosaccharide locus class of Campylobacter jejuni: sialylation is not needed for invasive infection

Ellström

Feodoroff

Hänninen

et al. 2014

Clinical Microbiology and Infection

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“…Although the outcome of Campylobacter gastroenteritis is typically good, infections have been occasionally linked to systemic invasive disease and postinfectious complications. On the basis of previous studies, the incidence of campylobacteraemia varies between 0.1% and 0.4% of the total incidence of Campylobacter infections [1][2][3]. However, clinical significance of campylobacteraemia is poorly understood, and it has been suspected to have been an underestimated event [1,4].…”

mentioning

confidence: 99%

“…Without a further study or change in routine reporting in Sweden, we will not be able to clarify who was affected, how severe these infections were and whether they were caused by a specific Campylobacter type. Interestingly, the most cases of campylobacteraemia in Finland have been shown to be caused by the single clonal complex ST-677 [3]. It would be important to consider typing of Campylobacter-positive blood isolates in Sweden to examine the prevalence of this potentially more blood-adapted type [5].…”

mentioning

confidence: 99%

Increased number of Campylobacter bacteraemia cases in Sweden, 2014

Harvala

Ydring

Brytting

et al. 2016

Clinical Microbiology and Infection

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Molecular epidemiology of Campylobacter jejuni infection in Israel—a nationwide study

Weinberger

Moran-Gilad

Rokney

et al. 2016

Clinical Microbiology and Infection

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The incidence of Campylobacter infection in Israel, particularly among children <2 years of age, has risen over the last decade and became one of the highest among industrialized countries. This study explored the molecular epidemiology of Campylobacter jejuni in Israel over a decade (2003-2012) using multilocus sequence typing (MLST) combined with demographic metadata. Representative clinical isolates (438) from a large national repository together with selected veterinary isolates (74) were subject to MLST. The distribution of age groups, ethnicity and clinical source across various genotypes was evaluated using Poisson modelling. The 512 studied isolates were assigned 126 distinct sequence types (STs) (18.8% novel STs) grouped into 21 clonal complexes (CCs). Most human, poultry and bovine STs clustered together in the leading CCs. Three dominant STs (ST21, ST6608, ST4766) were detected only since 2006. Patients infected with the leading CCs were similarly distributed along densely populated areas. The frequency of blood isolates was higher in patients infected with CC353 (relative rate (RR)=2.0, 95% CI 1.03-3.9, adjusted p value (adj.p) 0.047) and CC42 (RR=4.4, 95% CI 1.7-11.6, adj.p 0.018) and lower with CC257 (RR=0.3, 95% CI 0.1-0.9, adj. p 0.047). The distribution of age groups and ethnicity also varied across the leading CCs. In conclusion, C. jejuni isolates in a national sample appeared highly diverse with a high proportion of new STs. Phylogenic analysis was compatible with poultry and cattle as possible food sources of clinical infection. Demographic characteristics of the infected patients coupled with strain invasiveness across different genotypes revealed a complex epidemiology of C. jejuni transmission in Israel.

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Clonal Distribution and Virulence ofCampylobacter jejuniIsolates in Blood

Cited by 16 publications

References 12 publications

Lipooligosaccharide locus class of Campylobacter jejuni: sialylation is not needed for invasive infection

Lipooligosaccharide locus class of Campylobacter jejuni: sialylation is not needed for invasive infection

Increased number of Campylobacter bacteraemia cases in Sweden, 2014

Molecular epidemiology of Campylobacter jejuni infection in Israel—a nationwide study

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