Clonal propagation and production of cichoric acid in three species of Echinaceae

Butiuc-Keul, Anca; Vlase, Laurian; Crăciunaş, C.

doi:10.1007/s11627-012-9435-2

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…The lower limit of quantification was established at 0.75 µg/mL. At quantification limit, the method precision (expressed as coefficient of variation (CV) %) was 6.8%, and accuracy (expressed as relative difference between obtained and theoretical concentration, bias %) was 5.3%, respectively (R 2 > 0.999) [90].…”

Section: Hplc-uv-ms Separationmentioning

confidence: 99%

Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

Epure

Pârvu

Vlase

et al. 2020

Plants

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The present study analyzed the methanol extract and tincture obtained from the spontaneous Romanian Cichorium intybus species, in order to evaluate polyphenols content and some biological properties. Chromatographic and spectrophotometric methods were used for the analysis of polyphenols and the antioxidant capacity was assessed in vitro with DPPH● (2,2-diphenyl-picrylhydrazil) and FRAP (ferric-reducing antioxidant power) tests. The cardio-protective effects of Cichorii herba tincture on myocardial ischemia induced by isoprenaline and nephroprotection on renal failure induced by gentamicin were evaluated on rats. Also, aspartate aminotrasferase (AST), alanine aminotransferase (ALT), creatine kinase-MB (CK-MB) and creatinine clearance (CrCl) were measured. The antioxidant effect was evaluated by determining total oxidative stress (TOS), oxidative stress index (OSI, total antioxidant capacity (TAC), malondyaldehide (MDA), total thiols (SH) and total nitrites and nitrates (NOx). Cichoric acid was the main polyphenolic compound. The extracts had moderate in vitro antioxidant activity but the in vivo antioxidant and anti-inflammatory effects were significant and associated with myocardial and renal dysfunction improvement. The results were attributed to the content of polyphenols in the extracts, for which reason C. intybus may be considered an important raw material for pharmaceuticals formulations recommended in the prevention or treatment of heart or kidney diseases.

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Section: Hplc-uv-ms Separationmentioning

confidence: 99%

Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

Epure

Pârvu

Vlase

et al. 2020

Plants

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“…The advantage was a high throughput determination, having a major edge of rapid analysis m/z 293 + m/z 311, which is specific to cichoric acid. The QuantAnalysis 1.7 software (Brucker Daltonics, Darmstadt, Germany) instrumental data system was employed for the quantification of cichoric acid using peak area and the external standard method (R 2 > 0.999) [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

New Approaches on the Anti-Inflammatory and Cardioprotective Properties of Taraxacum officinale Tincture

et al. 2023

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The present research investigated the in vivo anti-inflammatory and cardioprotective activities, as well as the antioxidant potential of Taraxacum officinale tincture (TOT), in relation to the polyphenolic composition. Chromatographic and spectrophotometric techniques were used to determine the polyphenolic profile of TOT and the antioxidant activity was preliminarily assessed in vitro by DPPH• and FRAP spectrophotometric methods. The in vivo anti-inflammatory and cardioprotective activities were studied in rat turpentine-induced inflammation and in rat isoprenaline-induced myocardial infarction (MI) models. The main polyphenolic compound identified in TOT was cichoric acid. The oxidative stress determinations showed the capacity of the dandelion tincture not only to decrease the total oxidative stress (TOS), the oxidative stress index (OSI), and the total antioxidant capacity (TAC), but also the malondialdehide (MDA), thiols (SH), and nitrites/nitrates (NOx) levels both in inflammation and MI models. In addition, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatin kinase-MB (CK-MB), and nuclear factor kappa B (NF-κB) parameters were decreased by the administration of the tincture. The results show that T. officinale could be considered a valuable source of natural compounds with important benefits in pathologies linked to oxidative stress.

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“…Calibration curves in the 0.5 -50 µL/mL concentration range (R2 > 0.999) were used [29]. Cichoric acid was quantified using a newly optimised method of LC-MS previously described [10]. Cichoric acid was identified based on the retention time and the MS spectra compared to the standard.…”

Section: Methodsmentioning

confidence: 99%

Polyphenolic Compounds, Antioxidant Activity and Nephroprotective Properties of Romanian Taraxacum Officinale

Epure¹

2022

FARMACIA

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Taraxacum officinale (L.) Weber ex.F.H.Wigg. (dandelion) is a medicinal herb from the Asteraceae family, with a complex chemical composition. The purpose of this research was to determine the polyphenolic profile of Taraxaci herba tincture and to evaluate the antioxidant capacity and the nephroprotective activity. The polyphenolic compounds: total polyphenols content (TPC), total flavonoids content (TFC) and total caffeic acid derivates content (TCAD) were analysed by spectrophotometric and chromatographic methods. Some polyphenols were identified and quantified by HPLC-MS method, with cichoric acid as major compound. The evaluation of the in vitro antioxidant activity was performed using the DPPH• and FRAP methods. The nephroprotective activity of T. herba tincture was evaluated on a rat model with acute kidney injury (AKI) induced by gentamicin. Serum oxidative parameters (total oxidative stress -TOS, oxidative stress index -OSI, total antioxidant capacity -TAC, total nitrites and nitrates -NOx, malondyaldehide -MDA and total thiols -SH), kidney functional parameters (serum creatinine -SCr, serum urea -SU, urinary creatinine -Ucr, urinary urea -UU and creatinine clearance -ClCr) and the transcription factor (Nuclear Factor Kappa B) NF-kB were determined. Although the in vitro test results were modest, in vivo experiments showed a pronounced antioxidant capacity, associated with an important nephroprotective activity. RezumatTaraxacum officinale L. (păpădie) este o plantă medicinală din familia Asteraceae, cu o compoziție chimică foarte complexă. Scopul prezentei cercetări a fost determinarea profilului polifenolic al tincturii obținută din Taraxaci herba, precum și evaluarea capacității antioxidante și a activității nefroprotectoare. Compușii polifenolici -conținutul de polifenoli totali (TPC), conținutul de flavonoide totale (TFC) și conținutul de derivați de acid cafeic (TCAD) au fost analizați prin metode spectrofotometrice și cromatografice. Prin HPLC-MS s-au identificat și cuantificat compuși polifenolici, acidul cicoric fiind compusul majoritar. Evaluarea activității antioxidante in vitro s-a realizat prin metodele DPPH• și FRAP. Activitatea nefroprotectoare a tincturii de T. herba s-a evaluat pe șobolani de laborator cu insuficiență renală acută (AKI) indusă cu gentamicină. S-au evaluat parametri ai stresului oxidativ (status oxidativ total -TOS, index al stresului oxidativ -OSI, capacitatea antioxidantă totală -TAC, nivelul de nitriți și nitrați -NOx, malondialdehida -MDA, tioli -SH), parametri funcționali renali (creatinina serică -SCr, urea serică -SU, creatinina urinară -UCr, urea urinară -UU și clearance-ul creatininic -ClCr) și factorul de transcripție NF-kB. Deși rezultatele testărilor in vitro au fost modeste, experimentele in vivo au arătat o acțiune antioxidantă bună, asociată cu o importantă activitate nefroprotectoare.

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Clonal propagation and production of cichoric acid in three species of Echinaceae

Cited by 10 publications

References 31 publications

Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

New Approaches on the Anti-Inflammatory and Cardioprotective Properties of Taraxacum officinale Tincture

Polyphenolic Compounds, Antioxidant Activity and Nephroprotective Properties of Romanian Taraxacum Officinale

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