Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

Abstract

The present study analyzed the methanol extract and tincture obtained from the spontaneous Romanian Cichorium intybus species, in order to evaluate polyphenols content and some biological properties. Chromatographic and spectrophotometric methods were used for the analysis of polyphenols and the antioxidant capacity was assessed in vitro with DPPH● (2,2-diphenyl-picrylhydrazil) and FRAP (ferric-reducing antioxidant power) tests. The cardio-protective effects of Cichorii herba tincture on myocardial ischemia induced by isoprenaline and nephroprotection on renal failure induced by gentamicin were evaluated on rats. Also, aspartate aminotrasferase (AST), alanine aminotransferase (ALT), creatine kinase-MB (CK-MB) and creatinine clearance (CrCl) were measured. The antioxidant effect was evaluated by determining total oxidative stress (TOS), oxidative stress index (OSI, total antioxidant capacity (TAC), malondyaldehide (MDA), total thiols (SH) and total nitrites and nitrates (NOx). Cichoric acid was the main polyphenolic compound. The extracts had moderate in vitro antioxidant activity but the in vivo antioxidant and anti-inflammatory effects were significant and associated with myocardial and renal dysfunction improvement. The results were attributed to the content of polyphenols in the extracts, for which reason C. intybus may be considered an important raw material for pharmaceuticals formulations recommended in the prevention or treatment of heart or kidney diseases.

Possibilities of using leaves cultivated chicory (Cichorium intybus L.) as a medicinal plant material (review)

“…Выявлена способность исследуемых образцов цикория подавлять генерацию активных форм кислорода и перекисного окисления липидов, что характеризует их антиоксидантные свойства. Также проводились исследования по оценке антиоксидантной активности метанольного экстракта и настойки из травы цикория in vitro и in vivo для изучения их кардиозащитных и нефропротекторных свойств [28,[33][34][35][36].…”

Mechanistic Aspects of Apiaceae Family Spices in Ameliorating Alzheimer’s Disease

“…Plants are a rich source of bioactive compounds and display vast therapeutic properties ranging from antioxidative, anti-inflammatory, cardioprotective, nephron-protective, antidiabetic, antihypertensive, antimicrobial, etc. [14][15][16]. They have also been reported to exert neuroprotective properties by affecting multiple signaling pathways [17].…”

Spectrophotometric Evaluation of Flavonoids, Phenolcarboxylic Acids and Total Phenolic Contents of Several Indigenous Herbal Products With Potential Hepatoprotective Effect

“…Considering the scientific data, the aim of our paper was the evaluation of phenolic content of several indigenous herbal products -agrimony aerial parts (Agrimoniae herba), common cichory aerial parts (Cichorii herba), rosemary leaves (Rosmarini folium), dandelion aerial parts (Taraxaci herba) and artichoke leaves (Cynarae folium), in view of obtaining a phytomedicine with hepatoprotective effect. Indigenous herbal products were selected based on their chemical composition, rich in phenolic compounds [7,9,10,12,14].…”