Cloning and characterization of four novel SnRK2 genes from Triticum polonicum

Wang, Y.; Wang, X.; Gu, M.; Kang, Houyang; Zeng, Jian; Fan, Xiangtao; Li, Sha; Zhang, H.; Yu, Kangfu; Zhou, Yonghong

doi:10.1007/s10535-015-0501-6

Cited by 20 publications

(14 citation statements)

References 42 publications

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“…Total RNA was isolated from the stems of DPW and HPW under photoperiod treatments, and genomic DNA was removed using an RNase-free DNase set (Omega) according to the user manual. The qPCR and data analysis were performed as described by Wang et al 64 . Ten differentially expressed genes from RNA-Seq were validated, and their primers are listed in Additional file 4 : Supplementary Table 1.…”

Section: Methodsmentioning

confidence: 99%

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RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

Wang¹,

Xiao²,

Wang³

et al. 2016

Int. J. Biol. Sci.

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The dwarfing mechanism of Rht-dp in dwarf Polish wheat (DPW) is unknown. Each internode of DPW was significantly shorter than it in high Polish wheat (HPW), and the dwarfism was insensitive to photoperiod, abscisic acid (ABA), gibberellin (GA), cytokinin (CK), auxin and brassinolide (BR). To understand the mechanism, three sets of transcripts, DPW, HPW, and a chimeric set (a combination of DPW and HPW), were constructed using RNA sequencing (RNA-Seq). Based on the chimeric transcripts, 2,446 proteins were identified using isobaric tags for relative and absolute quantification (iTRAQ). A total of 108 unigenes and 12 proteins were considered as dwarfism-related differentially expressed genes (DEGs) and differentially expressed proteins (DEPs), respectively. Among of these DEGs and DEPs, 6 DEGs and 6 DEPs were found to be involved in flavonoid and S-adenosyl-methionine (SAM) metabolisms; 5 DEGs and 3 DEPs were involved in cellulose metabolism, cell wall plasticity and cell expansion; 2 DEGs were auxin transporters; 2 DEPs were histones; 1 DEP was a peroxidase. These DEGs and DEPs reduced lignin and cellulose contents, increased flavonoid content, possibly decreased S-adenosyl-methionine (SAM) and polyamine contents and increased S-adenosyl-L-homocysteine hydrolase (SAHH) content in DPW stems, which could limit auxin transport and reduce extensibility of the cell wall, finally limited cell expansion (the cell size of DPW was significantly smaller than HPW cells) and caused dwarfism in DPW.

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Section: Methodsmentioning

confidence: 99%

“…Ten differentially expressed genes from RNA-Seq were validated, and their primers are listed in Additional file 4 : Supplementary Table 1. Actin, as described by Wang et al 64 , was used as a reference gene to normalize gene expression.…”

Section: Methodsmentioning

confidence: 99%

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

Wang¹,

Xiao²,

Wang³

et al. 2016

Int. J. Biol. Sci.

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“…Quantitative real-time PCR with TpNRAMP3 -specific primers (forward: 5′-ACTCTGATGCTCCTGTTCCT-3′; reverse: 5′-GCCTCGCACAACTTCTGAA-3′) was performed as described by Wang et al (2015) with nine technological replicates of each sample. The actin gene ( Wang et al, 2015 ) was used as a reference gene to normalize relative expression level of TpNRAMP3 which was calculated by CFX Manager 3.1 (Bio-Rad, United States) using the 2 ΔΔ C t method.…”

Section: Methodsmentioning

confidence: 99%

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

et al. 2018

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Essential transition metals and non-essential metals often co-exist in arable soils. In plants, some transition metal transporters, such as the natural resistance-associated macrophage proteins (NRAMPs), poorly selectively transport metals with similar chemical properties whether they are essential or non-essential. In this study, a member of the NRAMP transporter family, TpNRAMP3, was identified from dwarf Polish wheat (Triticum polonicum L.). TpNRAMP3 encodes a plasma membrane-localized protein and was highly expressed in leaf blades and roots at the jointing and booting stages, and in the first nodes at the grain filling stage. Expression of TpNRAMP3 increased sensitivity to Cd and Co, but not Zn, and increased the Cd and Co concentrations in yeast. TpNRAMP3 expression in Arabidopsis increased concentrations of Cd, Co, and Mn, but not Fe or Zn, in roots, shoots, and whole plant. However, TpNRAMP3 did not affect translocation of Cd, Co, or Mn from roots to shoots. These results suggest that TpNRAMP3 is a transporter for Cd, Co, and Mn accumulation, but not for Fe or Zn. However, Cd and Co are non-essential toxic metals; selective genetic manipulation of TpNRAMP3 will help breed low Cd- and Co-accumulating cultivars.

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“…Quantitative real-time PCR was performed on the CFX-96 system as described by Wang et al using a pair of Rht-B1b-speci c primers (forward: 5′-GGCGGGAGATCGAAGTAC-3′; reverse: 5′-GACACCGTGCACTACAAC-3′) [34]. To normalize gene expression levels, the Actin gene was used as the reference gene [34]. Relative expression levels were calculated according to the 2 ΔΔCt method using the CFX Manager (version 3.1; Bio-Rad, Hercules, CA, USA).…”

Section: Expression Analysis Of Rht-b1bmentioning

confidence: 99%

The semi-dwarfing gene Rht-dp from dwarf Polish wheat (Triticum polonicum L.) is the “Green Revolution” gene Rht-B1b

Chai

Qin

Zhang

et al. 2020

Preprint

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Background The wheat dwarfing gene improves lodging resistance, and increases the grain number per spike and harvest index. Dwarf polish wheat (Triticum polonicum L., 2n = 4x = 28, AABB, DPW), initially collected from Tulufan, Xinjiang, China, carries a semi-dwarfing gene Rht-dp on chromosome 4BS. However, Rht-dp and its dwarfing mechanism are unknown. Results Homologous cloning and fine mapping revealed that Rht-dp is the ‘Green Revolution’ gene Rht-B1b. A haplotype analysis showed that Rht-B1b was only present in T. polonicum. Transcriptomic analysis of two pairs of near-isogenic lines (NILs) of DPW × TPW revealed 41 differentially expressed genes (DEGs) as potential dwarfism-related genes. Among them, 28 functionally annotated DEGs were classed into five sub-groups: hormone-related signalling transduction genes, transcription factor genes, cell wall structure-related genes, reactive oxygen-related genes, and nitrogen regulation-related genes. Conclusions These results indicated that Rht-dp is Rht-B1b, which regulates pathways related to hormones, reactive oxygen species, and nitrogen assimilation to modify the cell wall structure, and then limits cell wall loosening and inhibits cell elongation, thereby causing dwarfism in DPW.

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Cloning and characterization of four novel SnRK2 genes from Triticum polonicum

Cited by 20 publications

References 42 publications

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

The semi-dwarfing gene Rht-dp from dwarf Polish wheat (Triticum polonicum L.) is the “Green Revolution” gene Rht-B1b

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