Cloning and Characterization of Phosphomannomutase/Phosphoglucomutase (pmm/pgm) Gene of Vibrio anguillarum Related to Synthesis of LPS

Oh, Ryunkyoung; Moon, Soo Young; Cho, Hwa Jin; Jang, Won Je; Kim, Jang-Ho; Lee, Jong Min; Kong, In‐Soo

doi:10.4014/mbl.1607.07002

Cited by 2 publications

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“…Hence, considerable research has been undertaken to elucidate its underlying pathogenic virulence mechanisms and develop rapid detection techniques and effective disease prevention strategies. Despite these efforts, not much is known regarding V. anguillarum especially compared with that of human pathogenic Vibrio spp., including V. cholerae, V. parahaemolyticus, and V. vulni cus [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

Kim,

Park,

Rafquzzaman

et al. 2024

Preprint

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Vibrio anguillarum threatens fish and larval farming industries and human health worldwide. The fatty acids of cell membrane phospholipids are crucial to bacterial response to diverse environments and survival. Previously, short-chain dehydrogenase/reductase (SDR) was identified on chromosome II of the multichromosomal V. anguillarum. A comparison of SDR and the enzyme FabG-1b (encoded on chromosome I and responsible for the β-ketoacyl acyl carrier protein (ACP) reductase in fatty acid biosynthesis (FAS II)) showed that the amino acid sequence homology was only 33.2%; however, the core of functionality, which includes the NAD(P)-binding domain and the conserved region of the active site, the topologies predicted using sequence-based homology modeling, and the quaternary homotetramer-type structures showed a significant similarity. FabG-1b was specific to the substrates fluorinated and halogenated aliphatic ketones, aromatic ketones, and aromatic β-ketoesters and SDR toward non-fluorinated and non-halogenated aliphatic ketones, aromatic ketones, and non-aromatic β-ketoesters. This complementary catalytic efficiencies of the two enzymes on various substrates conclusively supports the hypothesis that the two enzymes are likely homologs. This is the first study to report potential paralogous enzymes FabG-1b and SDR in Vibrio. This information improves our understanding of bacterial FAS for establishing strategies to overcome infectious diseases caused by pathogenic strains and identify targets for developing new antibacterial agents.

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Section: Introductionmentioning

confidence: 99%

Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

Kim,

Park,

Rafquzzaman

et al. 2024

Preprint

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“…To date, we have attempted to expand our understanding of vibrio survival under extreme environmental stress through genetic and proteomic analyses of Vibrio anguillarum [19][20][21][22][23][24]. This study continues the search for speci c genetic resources from marine bacteria.…”

Section: Introductionmentioning

confidence: 99%

Genetic characteristics of novel extreme alkaline-inducible promoter located in five prime upstream region of peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum

Kim,

Oh,

Lee

et al. 2024

Preprint

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Vibrio has attracted attention as a promising genetic chassis in the field of synthetic biology. FK506 binding protein (FKBP)-type peptidyl-prolyl cis/trans isomerase (PPIase) is involved in protein folding. In this study, we report, for the first time, the promoter regions in Vibrio that drive increased FKBP expression in the extremely alkaline environment. Proteomic analysis of V. anguillarum NB10 showed that VaFKBP was significantly upregulated under extreme alkaline stress (pH 10) condition. Additionally, the putative core promoter-containing regions and a reporter gene coding a β-galactosidase were introduced into the Escherichia coli system, which showed β-galactosidase activity of 61.47 ± 2.91 and 95.83 ± 6.76 Miller unit (MU) at pH 9 and 10, respectively, after 4 h of stress. This outcome was 1.97- and 2.88-fold higher that that observed under normal conditions of 25°C and pH 7 (31.27 ± 1.15 MU). To the best of our knowledge, this is the first report of a promoter showing increased expression under extremely alkaline conditions. We believe that this is a useful chassis in promoter engineering and can be used as a powerful tool for activating transcriptionally silent biosynthetic gene clusters in specific environments.

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Cloning and Characterization of Phosphomannomutase/Phosphoglucomutase (pmm/pgm) Gene of Vibrio anguillarum Related to Synthesis of LPS

Cited by 2 publications

References 0 publications

Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

Homologous relationship between FabG involved in fatty acid biosynthesis and SDR on chromosome II in the multi-chromosome pathogen Vibrio anguillarum

Genetic characteristics of novel extreme alkaline-inducible promoter located in five prime upstream region of peptidyl-prolyl cis/trans isomerase from Vibrio anguillarum

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