Cloning and Characterization of the Promoter of the 9-<i>cis</i>-Epoxycarotenoid Dioxygenase Gene in<i>Arachis hypogaea</i>L.

Liang, Jianhua; Yang, Lixia; Xiong, Chen; Li, Ling; Guo, Dongliang; Li, Haihang; Zhang, Biyu

doi:10.1271/bbb.90133

Cited by 20 publications

(17 citation statements)

References 26 publications

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“…It has been reported that the NCED gene is induced by ABA application in Arabidopsis (Xiong et al 2002;Cheng et al 2002) and peanut (Wan and Li 2006;Liang et al 2009). The effect of exogenous ABA on AO expression has been reported in Arabidopsis (Cheng et al 2002;Barrero et al 2006) and tomato (Yesbergenova et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

et al. 2010

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An aldehyde oxidase gene, designated as aldehyde oxidase2 (AhAO2), was cloned from Arachis hypogaea L. The gene contained a 4,050-bp open reading frame that encoded a putative protein of 1,350 amino acids. The deduced amino acid sequence showed high identity with other plant AOs. The organ-specific expression pattern of AhAO2 has been examined, which indicates its dominant expression in leaves of peanut. The AhAO2 transcript level in leaves was greatly increased under exogenous ABA treatment for 1 h. Over-expression of AhAO2 in Arabidopsis led to an increase in ABA level and enhanced drought tolerance after drought treatment. These results indicated the possible involvement of AhAO2 in ABA synthesis and drought tolerance.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

et al. 2010

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“…ABRE is a conserved cis-regulatory element frequently found in promoters of genes involved in ABA responses, desiccation, and osmotic stress in a number of species (Liang et al, 2009;YamaguchiShinozaki and Shinozaki, 2005). We find it mainly in clusters of genes upregulated by singlet oxygen (Table 4), although it shows also a slight enrichment in clusters for other types of ROS.…”

Section: Distribution Of Known Cis-regulatory Elementsmentioning

confidence: 99%

Identification of cis-regulatory elements specific for different types of reactive oxygen species in Arabidopsis thaliana

Petrov

Vermeirssen

Clercq

et al. 2012

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“…Furthermore, the promoter region of AhNCED1 contains many abscisic acid-responsive elements (ABREs). Site-directed mutagenesis of two of these ABREs diminished AhNCED1 promoter activity [19], suggesting that ABA signaling pathways directly regulate AhNCED1 . The ABA-responsive element binding protein (AREB) gene AhAREB1 was cloned from peanut and overexpressed in Arabidopsis [16].…”

Section: Introductionmentioning

confidence: 99%

Identification of rapidly induced genes in the response of peanut (Arachis hypogaea) to water deficit and abscisic acid

Lü

Liu

et al. 2014

BMC Biotechnol

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BackgroundPeanut (Arachis hypogaea) is an important crop, but droughts often affect peanut production. There is a lack of genomic information available for peanut; therefore, little is known about the molecular basis of its drought stress response.ResultsPreviously, we found that peanut stomata close rapidly during water deficit and in response to abscisic acid (ABA) treatment, and many genes show changes in their expression levels. To screen for candidate genes involved in the water deficit response, we used the Illumina HiSeq2000/MiSeq sequencing platform to conduct a global transcriptome analysis of peanut seedlings under water deficit with or without an ABA pretreatment. Three peanut tissues (leaves, roots, and stems) collected at each of three developmental stages (four-leaf, flowering, and podding stages) were used to construct sequence libraries. Then, 4.96 × 107 raw sequence reads were generated and the high quality reads were assembled into 47,842 unigenes. We analyzed these sequence libraries to identify differentially expressed genes (DEGs) under water deficit with or without ABA pretreatment. In total, 621 genes were induced rapidly (≥1.5 fold change compared with control) under water deficit, 2,665 genes were induced rapidly under water deficit + ABA pretreatment, and 279 genes overlapped between water deficit and water deficit + ABA pretreatment. Of the 279 overlapping genes, 264 showed the same expression pattern and 15 showed opposite expression patterns. Among the DEGs, 257 were highly induced (>5 fold) by water deficit + ABA pretreatment, while 19 were highly induced (>5 fold) by water deficit alone. The genes induced under water deficit + ABA pretreatment included 100 putative transcription factor (TF) genes, while those induced under water deficit alone included only 22 putative TF genes. To validate the transcriptome results, we conducted quantitative PCR (qPCR) analyses to quantify the transcript levels of nine candidate genes.ConclusionsThe DEGs results show that many genes are rapidly induced in peanut in response to water deficit without or with ABA pretreatment. The results indicate that the main drought response mechanisms in peanut function through an ABA-dependent pathway. Our data provide a comprehensive sequence resource for molecular genetics research on peanut stress responses.

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Cloning and Characterization of the Promoter of the 9-cis-Epoxycarotenoid Dioxygenase Gene inArachis hypogaeaL.

Cited by 20 publications

References 26 publications

Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

Isolation and Characterization of the Aldehyde Oxidase2 Gene from Arachis hypogaea L.

Identification of cis-regulatory elements specific for different types of reactive oxygen species in Arabidopsis thaliana

Identification of rapidly induced genes in the response of peanut (Arachis hypogaea) to water deficit and abscisic acid

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