Cloning and characterization of two glutathione S-transferases from a DDT-resistant strain of <i>Anopheles gambiae</i>

Ranson, Hilary; Prapanthadara, La-aied; Hemingway, Janet

doi:10.1042/bj3240097

Cited by 128 publications

(125 citation statements)

References 30 publications

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“…GSTs have been associated with organochlorine and organophosphorous insecticide resistance both in the malaria mosquito (Anopheles gambiae) and in Drosophila (28)(29)(30)(31). We found five GST transcripts overexpressed in the DDT-resistant lines ( Fig.…”

Section: Resultsmentioning

confidence: 83%

Genome-wide transcription profile of field- and laboratory-selected dichlorodiphenyltrichloroethane (DDT)-resistant Drosophila

Pedra

McIntyre

Scharf

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

213

268

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Genome-wide microarray analysis (Affymetrix array) was used (i) to determine whether only one gene, the cytochrome P450 enzyme Cyp6g1, is differentially transcribed in dichlorodiphenyltrichloroethane (DDT)-resistant vs. -susceptible Drosophila; and (ii) to profile common genes differentially transcribed across a DDT-resistant field isolate [Rst(2)DDT Wisconsin ] and a laboratory DDT-selected population [Rst(2)DDT 91-R ]. Statistical analysis (ANOVA model) identified 158 probe sets that were differentially transcribed among Rst(2)DDT 91-R , Rst(2)DDT Wisconsin , and the DDT-susceptible genotype Canton-S (P < 0.01). The cytochrome P450 Cyp6a2 and the diazepam-binding inhibitor gene (Dbi) were over transcribed in the two DDT-resistant genotypes when compared to the wild-type Drosophila, and this difference was significant at the most stringent statistical level, a Bonferroni correction. The list of potential candidates differentially transcribed also includes 63 probe sets for which molecular function ontology annotation of the probe sets did not exist. A total of four genes (Cyp6a2, Dbi, Uhg1, and CG11176) were significantly different (P < 5.6 e ؊06 ) between Rst(2)DDT 91-R and Canton-S. Additionally, two probe sets encoding Cyp12d1 and Dbi were significantly different between Rst(2)DDT Wisconsin and Canton-S after a Bonferroni correction. Fifty-two probe sets, including those associated with pesticide detoxification, ion transport, signal transduction, RNA transcription, and lipid metabolism, were commonly expressed in both resistant lines but were differentially transcribed in Canton-S. Our results suggest that more than Cyp6g1 is overtranscribed in field and laboratory DDT-resistant genotypes, and the number of commonalities suggests that similar resistance mechanisms may exist between laboratory-and field-selected DDT-resistant fly lines.T he evolution of insecticide resistance is often, but not always, based on major effect alleles (1-4). It has been hypothesized that high selection pressure in the field will favor monogenic forms of pesticide resistance, and that selection for resistance in the laboratory will favor polygenic resistance (5-7). In early genetic studies in Drosophila, dichlorodiphenyltrichloroethane (DDT) resistance was mapped to multiple locations on chromosomes II and III (8-14). Subsequently, low-level DDT resistance was mapped to 64.5 Ϯ 2 centiMorgans on the second chromosome (15), a locus (loci) known as Rst(2)DDT.Recently, Daborn et al. (16) suggested that resistance to DDT in the field is monogenic and is due to the overexpression of a single P450 gene, Cyp6g1. Le Goff et al. (17) suggested that resistance in field isolates of both Drosophila melanogaster and Drosophila simulans is associated with overtranscription of Cyp6g1, whereas prolonged laboratory selection with DDT apparently coselects additional genes such as Cyp12d1 (18) To date, no genome-wide expression profile has been evaluated to investigate the extent to which gene transcription varies between genotypes that are resis...

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Section: Resultsmentioning

confidence: 83%

Genome-wide transcription profile of field- and laboratory-selected dichlorodiphenyltrichloroethane (DDT)-resistant Drosophila

Pedra

McIntyre

Scharf

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

213

268

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“…Exposure to anticancer agents may lead to the induction and expression of gene products that protect the cell. GSTs have been implicated in the development of resistance toward chemotherapy agents, insecticides, herbicides, and microbial antibiotics (Tew, 1994;McLellan and Wolf 1999;Tang and Tu, 1994;Ranson et al, 1997). It is plausible that GSTs serve two distinct roles in the development of drug resistance via direct detoxification as well as acting as an inhibitor of the MAP kinase pathway.…”

Section: Resistancementioning

confidence: 99%

The role of glutathione-S-transferase in anti-cancer drug resistance

2003

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“…GST implicated in DDT insecticide resistance exist as clusters of genes that have been further shuffled through the genome by recombination (40). A number of resistance GST genes, including multiple forms in the same insect, have been characterized in vectors (41)(42)(43).…”

Section: B Amentioning

confidence: 99%