Cloning and expression of the rabbit prostaglandin EP2 receptor

Guan, Youfei; Stillman, Brett A.; Zhang, Yahua; Schneider, André; Saito, Osamu; Davis, Linda S.; Redha, Reyadh; Breyer, Richard M.; Breyer, Matthew D.

doi:10.1186/1471-2210-2-14

Cited by 23 publications

(7 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…24 In the same study, it was reported that EP 2 receptor mRNA could be detected in cultured renal medullary interstitial cells. 24 In addition, in rat kidney, it has been shown that the EP 2 receptor is mainly expressed in the descending thin limb of the loop of Henle and the vasa recta of the outer medulla. 25 The described tissue expression is in line with the main function contributed to the EP 2 receptor, namely renal salt and water handling.…”

Section: Stimulation Of the Ep 2 Receptor Mitigates Fibrogenesis In H...mentioning

confidence: 89%

Activation of the prostaglandin E₂ EP₂ receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

et al. 2019

View full text Add to dashboard Cite

Aim Renal fibrosis plays a pivotal role in the development and progression of chronic kidney disease, which affects 10% of the adult population. Previously, it has been demonstrated that the cyclooxygenase‐2 (COX‐2)/prostaglandin (PG) system influences the progression of renal injury. Here, we evaluated the impact of butaprost, a selective EP2 receptor agonist, on renal fibrosis in several models of kidney injury, including human tissue slices. Methods We studied the anti‐fibrotic efficacy of butaprost using Madin‐Darby Canine Kidney (MDCK) cells, mice that underwent unilateral ureteral obstruction and human precision‐cut kidney slices. Fibrogenesis was evaluated on a gene and protein level by qPCR and Western blotting. Results Butaprost (50 μM) reduced TGF‐β‐induced fibronectin (FN) expression, Smad2 phosphorylation and epithelial‐mesenchymal transition in MDCK cells. In addition, treatment with 4 mg/kg/day butaprost attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery, as illustrated by a reduction in the gene and protein expression of α‐smooth muscle actin, FN and collagen 1A1. More importantly, a similar anti‐fibrotic effect of butaprost was observed in human precision‐cut kidney slices exposed to TGF‐β. The mechanism of action of butaprost appeared to be a direct effect on TGF‐β/Smad signalling, which was independent of the cAMP/PKA pathway. Conclusion In conclusion, this study demonstrates that stimulation of the EP2 receptor effectively mitigates renal fibrogenesis in various fibrosis models. These findings warrant further research into the clinical application of butaprost, or other EP2 agonists, for the inhibition of renal fibrosis.

show abstract

Section: Stimulation Of the Ep 2 Receptor Mitigates Fibrogenesis In H...mentioning

confidence: 89%

Activation of the prostaglandin E₂ EP₂ receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Indeed, mRNAs for the three PPAR isotypes have been found in the normal ureter and bladder (52). Recently, PPAR␥ has been reported to be highly expressed in human TCCs as well as in the T24 cell line (46).…”

Section: Discussionmentioning

confidence: 99%

Differential Regulation of Vascular Endothelial Growth Factor Expression by Peroxisome Proliferator-activated Receptors in Bladder Cancer Cells

Fauconnet¹,

Lascombe²,

Chabannes³

et al. 2002

Journal of Biological Chemistry

107

View full text Add to dashboard Cite

The growth of any solid tumor depends on angiogenesis. Vascular endothelial growth factor (VEGF) plays a prominent role in vesical tumor angiogenesis regulation. Previous studies have shown that the peroxisome proliferator-activated receptor ␥ (PPAR␥) was involved in the angiogenesis process. Here, we report for the first time that in two different human bladder cancer cell lines, RT4 (derived from grade I tumor) and T24 (derived from grade III tumor), VEGF (mRNA and protein) is differentially up-regulated by the three PPAR isotypes. Its expression is increased by PPAR␣, ␤, and ␥ in RT4 cells and only by PPAR␤ in T24 cells via a transcriptional activation of the VEGF promoter through an indirect mechanism. This effect is potentiated by an RXR (retinoid-X-receptor), selective retinoid LG10068 providing support for a PPAR agonist-specific action on VEGF expression. While investigating the downstream signaling pathways involved in PPAR agonist-mediated up-regulation of VEGF, we found that only the MEK inhibitor PD98059 reduced PPAR ligand-induced expression of VEGF. These data contribute to a better understanding of the mechanisms by which PPARs regulate VEGF expression. They may lead to a new therapeutic approach to human bladder cancer in which excessive angiogenesis is a negative prognostic factor.

show abstract

“…The EP2 receptor is detected in vascular smooth muscle cells and renal interstitial cells. Additionally, EP2 is also expressed in glomeruli, the descending thin limb of the loop of Henle, and cortical and outer medullary collecting ducts as shown by reverse transcription polymerase chain reaction and functional studies [ 47 – 50 ]. EP2 stimulates adenylate cyclase and signals via the secondary messenger cyclic adenosine monophosphate (cAMP) [ 26 ].…”

Section: Prostaglandin E2mentioning

confidence: 99%

Prostaglandin E2 receptors as therapeutic targets in renal fibrosis

Mutsaers

Nørregaard

2022

Kidney Res Clin Pract

View full text Add to dashboard Cite

Prostaglandin E2 (PGE2), a lipid mediator produced by the cyclooxygenase enzyme system, is the main prostaglandin in the kidney. PGE2 is involved in various physiological and pathophysiological processes in the kidney, including renal hemodynamics, water and salt balance, and renal fibrosis—a key pathological feature of progressive kidney diseases. PGE2 functions by binding to four G-protein-coupled EP receptors (EP1 to EP4), which stimulate different intracellular signaling pathways. The intrarenal distribution of the four EP receptors as well as the different downstream signaling pathways associated with each receptor give rise to the distinct functional consequence of activating each receptor subtype. This review summarizes the current data on the renal expression of the four EP receptors and delineates the role of each receptor in renal fibrosis.

show abstract

Cloning and expression of the rabbit prostaglandin EP2 receptor

Abstract: Background: Prostaglandin E 2 (PGE 2 ) has multiple physiologic roles mediated by G protein coupled receptors designated E-prostanoid, or "EP" receptors. Evidence supports an important role for the EP 2 receptor in regulating fertility, vascular tone and renal function.

Cited by 23 publications

References 48 publications

Activation of the prostaglandin E₂ EP₂ receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

Activation of the prostaglandin E₂ EP₂ receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

Differential Regulation of Vascular Endothelial Growth Factor Expression by Peroxisome Proliferator-activated Receptors in Bladder Cancer Cells

Prostaglandin E2 receptors as therapeutic targets in renal fibrosis

Contact Info

Product

Resources

About

Cloning and expression of the rabbit prostaglandin EP2 receptor

Abstract: Background: Prostaglandin E 2 (PGE 2 ) has multiple physiologic roles mediated by G protein coupled receptors designated E-prostanoid, or "EP" receptors. Evidence supports an important role for the EP 2 receptor in regulating fertility, vascular tone and renal function.

Cited by 23 publications

References 48 publications

Activation of the prostaglandin E2 EP2 receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

Activation of the prostaglandin E2 EP2 receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

Differential Regulation of Vascular Endothelial Growth Factor Expression by Peroxisome Proliferator-activated Receptors in Bladder Cancer Cells

Prostaglandin E2 receptors as therapeutic targets in renal fibrosis

Contact Info

Product

Resources

About

Activation of the prostaglandin E₂ EP₂ receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices

Activation of the prostaglandin E₂ EP₂ receptor attenuates renal fibrosis in unilateral ureteral obstructed mice and human kidney slices