Cloning and functional analysis of a laccase gene during fruiting body formation in Hypsizygus marmoreus

Zhang, Jinjing; Chen, Hui; Chen, Mingjie; Ren, Ang; Huang, Jianchun; Wang, Hong; Zhao, Mingwen; Feng, Zhen

doi:10.1016/j.micres.2015.06.005

Cited by 53 publications

(42 citation statements)

References 36 publications

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“…A siRNA knockdown study demonstrates that Lcc2 in A. bisporus contributes to toxin metabolism and defense against green mold disease (Sjaarda et al, 2015). On the other hand, overexpression of Hypsizygus marmoreus Lcc1 facilitates mycelial growth and fruiting body initiation (Zhang et al, 2015). Indeed, laccase has been developed as a novel screening marker in mushroom breeding (Sun et al, 2014).…”

Section: Natural Laccase Producersmentioning

confidence: 99%

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

et al. 2017

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Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas. There have been over two dozen reviews on laccases since 2010 covering various aspects of this group of versatile enzymes, from their occurrence, biochemical properties, and expression to immobilization and applications. This review is not intended to be all-encompassing; instead, we highlighted some of the latest developments in basic and applied laccase research with an emphasis on laccase-mediated bioremediation of pharmaceuticals, especially antibiotics. Pharmaceuticals are a broad class of emerging organic contaminants that are recalcitrant and prevalent. The recent surge in the relevant literature justifies a short review on the topic. Since low laccase yields in natural and genetically modified hosts constitute a bottleneck to industrial-scale applications, we also accentuated a genus of laccase-producing white-rot fungi, Cerrena, and included a discussion with regards to regulation of laccase expression.

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Section: Natural Laccase Producersmentioning

confidence: 99%

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

et al. 2017

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“…Recently, the mechanisms of this process have been extensively studied. For instance, the studies on Pleurotus tuoliensis [3], Priurotus eryngii [4], Lentinula edodes [5], Schizophyllum commune [6], Flammulina velutipes [7], Ganoderma lucidum [8], Cordyceps militaris [9], Hypsizygus marmoreus [10,11], Termitomyces heimii [12], Coprinopsis cinerea [2,13], and Pleurotus ostreatus [14], found some key genes that regulated mushroom development, and demonstrated the underlying molecular mechanisms in this process. However, studies on the mechanisms of fruiting body development in Sparassis latifolia are still at a primary stage.…”

Section: Introductionmentioning

confidence: 99%

Integration of ATAC-Seq and RNA-Seq Identifies Key Genes in Light-Induced Primordia Formation of Sparassis latifolia

Yang

Xiao

et al. 2019

IJMS

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Light is an essential environmental factor for Sparassis latifolia primordia formation, but the molecular mechanism is still unclear. In this study, differential expression profiling of light-induced primordia formation (LIPF) was established by integrating the assay for transposase accessible chromatin by sequencing (ATAC-seq) and RNA-seq technology. The integrated results from the ATAC-seq and RNA-seq showed 13 down-regulated genes and 17 up-regulated genes in both the L vs. D and P vs. D groups, for both methods. According to the gene ontology (GO) annotation of these differentially expressed genes (DEGs), the top three biological process categories were cysteine biosynthetic process via cystathionine, vitamin B6 catabolic, and glycine metabolic; the top three molecular function categories were 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity, glycine binding, and pyridoxal phosphate binding; cellular component categories were significantly enriched in the glycine cleavage complex. The KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis revealed that these genes were associated with vitamin B6 metabolism; selenocompound metabolism; cysteine and methionine metabolism; glycine, serine, and threonine metabolism; and glyoxylate and dicarboxylate metabolism pathways. The expression of most of the DEGs was validated by qRT-PCR. To the best of our knowledge, this study is the first integrative analysis of ATAC-seq and RNA-seq for macro-fungi. These results provided a new perspective on the understanding of key pathways and hub genes in LIPF in S. latifolia. It will be helpful in understanding the primary environmental response, and provides new information to the existing models of primordia formation in edible and medicinal fungi.

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“…One enhancer‐like element involved in anoxic‐specific inducibility and one circadian cis ‐acting regulatory element involved in circadian control were identified, suggesting that transcription of the Fvsdh gene might be associated with F . velutipes development .…”

Section: Discussionmentioning

confidence: 99%

Overexpression of the saccharopine dehydrogenase gene improves lysine biosynthesis inFlammulina velutipes

Liu

Jiang

et al. 2019

J Basic Microbiol

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Saccharopine dehydrogenase (EC 1.5.1.7) regulates the last step of fungal lysine biosynthesis. The gene (Fvsdh) encoding saccharopine dehydrogenase was identified and cloned from the whole genome of Flammulina velutipes. The genomic DNA of Fvsdh is 1257 bp, comprising three introns and four exons. The full-length complementary DNA of Fvsdh comprises 1107 bp with a deduced amino acid sequence of 368 residues. A 1,000-bp promoter sequence containing the TATA box, CAAT box, and several putative cis-acting elements was also identified. The results of tissue expression analysis showed that the expression level of the Fvsdh gene was higher in the pileus than in the stipe whether in the elongation or maturation stage. Further research showed that the lysine contents were 3.03 and 2.95 mg/g in maturation-pileus and elongation-pileus, respectively. In contrast, the lysine contents were 2.49 and 2.07 mg/g in elongation-stipe and maturation-stipe, respectively. To study the function of Fvsdh, we overexpressed Fvsdh in F. velutipes and found that Fvsdh gene expression was increased from 1.1-to 3-fold in randomly selected transgenic strains. The lysine contents were also increased from 1.12-to 1.3-fold in these five transformants, except for strain T3, in which the lysine contents were the same as the control. These results indicate that the expression of the Fvsdh gene can affect the lysine content of F. velutipes. K E Y W O R D SFlammulina velutipes, lysine content, saccharopine dehydrogenase

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Cloning and functional analysis of a laccase gene during fruiting body formation in Hypsizygus marmoreus

Cited by 53 publications

References 36 publications

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Integration of ATAC-Seq and RNA-Seq Identifies Key Genes in Light-Induced Primordia Formation of Sparassis latifolia

Overexpression of the saccharopine dehydrogenase gene improves lysine biosynthesis inFlammulina velutipes

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