Cloning and sequencing of 16S rDNA and 16S-23S rDNA internal spacer region (ISR) from urease-positive thermophilic Campylobacter (UPTC)

Miyajima, Michio; Matsuda, Motoo; Haga, Setsuko; Kagawa, Shizuko; Millar, B. Cherie; Moore, John E.

doi:10.1046/j.1472-765x.2002.01082.x

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…PCR amplification, cloning and sequencing of the nearly full-length 16S rDNA have also been described [ 15 ]. In relation to the PCR, at present, a primer pair set (fD1; 5'-GAATTTGATCCTGGCTCAG-3' and rTel; 5'-GGCTACCTTGTTACGACTT-3') was employed for amplification of the nearly full-length 16S rDNA from T. equigenitalis strains.…”

Section: Methodsmentioning

confidence: 99%

Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis

et al. 2006

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BackgroundAt present, six accessible sequences of 16S rDNA from Taylorella equigenitalis (T. equigenitalis) are available, whose sequence differences occur at a few nucleotide positions. Thus it is important to determine these sequences from additional strains in other countries, if possible, in order to clarify any anomalies regarding 16S rDNA sequence heterogeneity. Here, we clone and sequence the approximate full-length 16S rDNA from additional strains of T. equigenitalis isolated in Japan, Australia and France and compare these sequences to the existing published sequences.ResultsClarification of any anomalies regarding 16S rDNA sequence heterogeneity of T. equigenitalis was carried out. When cloning, sequencing and comparison of the approximate full-length 16S rDNA from 17 strains of T. equigenitalis isolated in Japan, Australia and France, nucleotide sequence differences were demonstrated at the six loci in the 1,469 nucleotide sequence. Moreover, 12 polymorphic sites occurred among 23 sequences of the 16S rDNA, including the six reference sequences.ConclusionHigh sequence similarity (99.5% or more) was observed throughout, except from nucleotide positions 138 to 501 where substitutions and deletions were noted.

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Section: Methodsmentioning

confidence: 99%

Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis

et al. 2006

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“…Consequently, to compare Arcobacter sequences with those of other Epsilonproteobacteria, it would be ideal to have an alternative support to 16S rRNA gene. Therefore, other target genes have been considered already: 23S rRNA gene (Miyajima et al, 2002;Dewhirst et al, 2005), (19 A. butzleri and 2 A. cryaerophilus) isolated from human clinical specimens, essentially feces, in 2003 and 2004 from all over France, and sent to the French National Reference Center for Campylobacter and Helicobacter. All strains were grown as previously reported (Prouzet-Mauléon et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence of thegyrAgene ofArcobacterspecies and characterization of human ciprofloxacin-resistant clinical isolates

Abdelbaqi¹,

Ménard

Prouzet‐Mauléon³

et al. 2007

FEMS Immunology &Amp; Medical Microbiology

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The nucleotide sequence of the gyrA gene of Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter cibarius, and Arcobacter skirrowii was determined. The deduced GyrA proteins are closely related to those of Wolinella succinogenes and Helicobacter pullorum, whereas those of Campylobacter species showed less sequence identity. The phylogenetic analysis of GyrA sequences provides a result similar to 16S rRNA gene sequence phylogenetic analysis and allows the discrimination among A. butzleri species. In addition, a Thr-->Ile mutation at amino acid 85 in the quinolone resistance-determining region was associated with ciprofloxacin resistance for two A. butzleri and one A. cryaerophilus ciprofloxacin-resistant strains.

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“…1). A novel primer pair (f-Cl23R1 and r-Cl23R1) was designed based on sequence information of the 16S-23S rDNA internal spacer region (ISR) from UPTC CF89-12 [29] and the rRNA operon A of C. jejuni TGH9011 (ATCC43431) [30]. Three other novel primer pairs (f-Cl23Rin and r-Cl23Rin; f-Cl23h25 and r-Cl23h25; f-Cl23h45 and r-Cl23h45) were also constructed based on the sequence information described above and the 23S RNA gene sequence information from C. jejuni TGH9011 (Z29326) and C. coli VC167 (U09611) ( Fig.…”

Section: Template Dna Preparation Primer Design and Pcr Amplificationmentioning

confidence: 99%

Demonstration of the absence of intervening sequences within 23S rRNA genes from Campylobacter lari

Tazumi¹,

Kakinuma²,

Moore³

et al. 2009

J. Basic Microbiol.

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Cloning, sequencing and characterization of nearly full-length 23S rRNA genes in 12 urease-positive thermophilic Campylobacter (UPTC) isolates were carried out using two novel PCR primer pairs. Nucleotide sequences of the 23S rRNA genes from the 12 isolates were first shown not to carry any intervening sequences (IVSs) in both the 25 and 45 helix regions. Then, two PCR primer sets were designed in silico for amplification of the helix 25 and 45 regions within 23S rRNA gene sequences from Campylobacter lari. No IVSs were identified within the 23S rRNA genes among a total of 53 isolates of C. lari, following PCR amplification, TA cloning and sequencing procedures. Intact 23S rRNA was identified in all 65 C. lari isolates, resulting in no production of the fragmented 23S rRNA. These data suggest that C. lari may not have any opportunity to interact with any other source of IVSs until now, or has been unable to integrate IVSs into their own genomes.

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Cloning and sequencing of 16S rDNA and 16S-23S rDNA internal spacer region (ISR) from urease-positive thermophilic Campylobacter (UPTC)

Cited by 9 publications

References 20 publications

Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis

Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis

Nucleotide sequence of thegyrAgene ofArcobacterspecies and characterization of human ciprofloxacin-resistant clinical isolates

Demonstration of the absence of intervening sequences within 23S rRNA genes from Campylobacter lari

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