Cloning of an Enzyme That Synthesizes a Key Nucleotide-Sugar Precursor of Hemicellulose Biosynthesis from Soybean:UDP-Glucose Dehydrogenase

Tenhaken, Raimund; Thulke, Oliver

doi:10.1104/pp.112.3.1127

Cited by 114 publications

(103 citation statements)

References 30 publications

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“…1). In the more prominent pathway, UDP-glucose is oxidized directly into UDP-GlcA by the enzyme UDP-glucose dehydrogenase (UGD; Tenhaken and Thulke 1996). This pathway is often the only one mentioned in plant biochemistry textbooks.…”

Section: Introductionmentioning

confidence: 99%

The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides

Kanter

Usadel

Guérineau

et al. 2005

Planta

137

141

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The nucleotide sugar UDP-glucuronic acid (UDP-GlcA) is the principal precursor for galacturonic acid, xylose, apiose and arabinose residues of the plant cell-wall polymers. UDP-GlcA can be synthesized by two different functional pathways in Arabidopsis involving either UDP-glucose dehydrogenase or inositol oxygenase as the initial enzyme reaction to channel carbohydrates into a pool of UDP sugars used for cellwall biosynthesis. The genes for the enzyme myo-inositol oxygenase (MIOX) were analyzed in Arabidopsis. They represent a small gene family containing four members. The transcription of all those members indicates a transient and organ-specific gene expression pattern in growing plant tissues as analyzed by RT-PCR and in promoter::GUS reporter gene lines. Two isoforms (MIOX1, MIOX2) are expressed in almost all tissues of the plant, whereas the expression of MIOX4 and MIOX5 is largely restricted to flowers, particularly maturing pollen. T-DNA insertion lines in MIOX genes were isolated; however, single knock-outs show growth phenotypes similar to the wild type. The monosaccharide composition of the cell wall in these mutants is not significantly changed compared to wild type plants. However, the incorporation of 3 H-inositol into wall polymers of seedlings is greatly impaired in the mutant lines DMIOX1 and DMIOX2, which are the only isoforms that are expressed in seedlings.

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Section: Introductionmentioning

confidence: 99%

The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides

Kanter

Usadel

Guérineau

et al. 2005

Planta

137

141

View full text Add to dashboard Cite

show abstract

“…Phe may be transported from phloem or be synthesized in the cells. Precursors for hemicelluloses are derived from UDP-Glc (UDPG) through oxidation by UDPGlc dehydrogenase (UDPG-DH; Tenhaken and Thulke, 1996). aa, Amino acids; DHAP, 3-deoxy-Darabino-heptulosonate 7-phosphate; E4P, erythrose 4-P; G6P, Glc-6-P; OPP, oxidative pentose phosphate; PEP, phosphoenolpyruvate.…”

Section: Transcription Factors and Lignin Biosynthesismentioning

confidence: 99%

Lignin and Biomass: A Negative Correlation for Wood Formation and Lignin Content in Trees

et al. 2010

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“…The gene for UDP-glucose epimerase has been isolated from Arabidopsis (Dormann and Benning, 1996), and the UDP-glucose dehydrogenase gene has been isolated from soybean (Glycine max (L.) Merr.) (Tenhaken and Thulke, 1996). These enzymes convert UDP-glucose to UDP-galactose and UDP-glucuronic acid, respectively.…”

Section: Other Cell Wall Polysaccharidesmentioning

confidence: 99%

Genetic Modification of Herbaceous Plants for Feed and Fuel

Vogel

Jung

2001

Critical Reviews in Plant Sciences

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Abstract:Much of the research on the genetic modification of herbaceous plant cell walls has been conducted to improve the utilization of forages by ruminant livestock. The rumen of these animals is basically an anaerobic fermentation vat in which the microflora break down the complex polysaccharides of plant cell walls into simpler compounds that can be further digested and absorbed by the mammalian digestive system. Research on improving the forage digestibility of switchgrass, Panicum virgatum L., and other herbaceous species has demonstrated that genetic improvements can be made in forage quality that can have significant economic value. To meet future energy needs, herbaceous biomass will need to be converted into a liquid fuel, probably ethanol, via conversion technologies still under development. If feedstock quality can be genetically improved, the economics and efficiency of the conversion processes could be significantly enhanced. Improving an agricultural product for improved end product use via genetic modification requires knowledge of desired quality attributes, the relative economic value of the quality parameters in relation to yield, genetic variation for the desired traits, or for molecular breeding, knowledge of genes to suppress or add, and knowledge of any associated negative consequences of genetic manipulation. Because conversion technology is still under development, desirable plant feedstock characteristics have not been completely delineated. Some traits such as cellulose and lignin concentration will undoubtably be important. Once traits that affect biomass feedstock conversion are identified, it will be highly feasible to genetically modify the feedstock quality of herbaceous plants using both conventional and molecular breeding techniques. The use of molecular markers and transformation technology will greatly enhance the capability of breeders to modify the morphologic structure and cell walls of herbaceous species. It will be necessary to monitor gene flow to remnant wild populations of biomass plants and have strategies available to curtail gene flow if it becomes a potential problem. It will also be necessary to monitor plant survival and longterm productivity as affected by these genetic changes to herbaceous species.

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Cloning of an Enzyme That Synthesizes a Key Nucleotide-Sugar Precursor of Hemicellulose Biosynthesis from Soybean:UDP-Glucose Dehydrogenase

Cited by 114 publications

References 30 publications

The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides

The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides

Lignin and Biomass: A Negative Correlation for Wood Formation and Lignin Content in Trees

Genetic Modification of Herbaceous Plants for Feed and Fuel

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