Cloning of profilin (FcPFN) from the shrimpFenneropenaeus chinensis, a highly expressed protein in white spot syndrome virus (WSSV)-infected shrimp

Abstract

We isolated and characterized the profilin (FcPFN) cDNA from hemocytes of Fenneropenaeus chinensis, a unique shrimp species from the Yellow Sea. The FcPFN cDNA consists of 830 bp and encodes a polypeptide of 125 amino acids, having a predicted isoelectric point of 5.06. The deduced amino acid sequence of FcPFN shows 36% and 90% amino acid sequence identity to the profilin genes of Pacific white shrimp Litopenaeus vannamei and black tiger shrimp Penaeus monodon, respectively. The FcPFN mRNA was highly expressed in hemocytes and hepatopancreas and moderately in muscle of normal shrimp. The higher expression of FcPFN mRNA is observed in shrimp infected with the white spot syndrome virus (WSSV), which is a major concern in all shrimp-growing regions of the world. These results suggest a potential role for FcPFN in viral host defense mechanisms.

Molecular Cloning and Bioinformatics Analysis of Shrimp Lvprofilin Implicated in Muscle Formation and Muscle Specific Gene Regulation

“…Overall, LvProfilin is conserved both DNA (Figure 3a) and protein (Figure 3b) confirming the BLAST result, especially in the Nterminal locating of poly-proline binding site involving in profilin signaling of actin formation [8,9]. Besides the actin binding site is found in the middle and C-terminal (Figure 3b) [10], several other amino acids are conserved in all species examined thus far (dark shading). These conserved domains were examined for the specific function using several bioinformatics tools.…”