Cloning of α-β fusion gene from Clostridium perfringens and its expression

Bai, Jianing; Yan, Zhang; Zhao, Baisuo

doi:10.3748/wjg.v12.i8.1229

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…A study with the region CPB (143–311) , which was fused to the C -terminal part of CPI (CPI-C (466–665) ), showed that the resulting protein (called rCPIB) was able to protect 83% and 91% of mice challenged with 5 × LD 100 of CPI and CPB, respectively [70]. Similarly, Bai et al [73] produced a bivalent chimera that contained CPA and CPB (rCPAB), which was able to protect 100% (10/10) of mice challenged with 1 L+ of CPB. Another chimeric antigen with ETX and CPB (rETXCPB) was able to generate titers of 6 and 10 IU/mL against the respective toxins in rabbits [71].…”

Section: Beta Toxin (Cpb)mentioning

confidence: 99%

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Ferreira

Moreira

Cunha

et al. 2016

Toxins

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Clostridium perfringens is a spore-forming, commensal, ubiquitous bacterium that is present in the gastrointestinal tract of healthy humans and animals. This bacterium produces up to 18 toxins. The species is classified into five toxinotypes (A–E) according to the toxins that the bacterium produces: alpha, beta, epsilon, or iota. Each of these toxinotypes is associated with myriad different, frequently fatal, illnesses that affect a range of farm animals and humans. Alpha, beta, and epsilon toxins are the main causes of disease. Vaccinations that generate neutralizing antibodies are the most common prophylactic measures that are currently in use. These vaccines consist of toxoids that are obtained from C. perfringens cultures. Recombinant vaccines offer several advantages over conventional toxoids, especially in terms of the production process. As such, they are steadily gaining ground as a promising vaccination solution. This review discusses the main strategies that are currently used to produce recombinant vaccines containing alpha, beta, and epsilon toxins of C. perfringens, as well as the potential application of these molecules as vaccines for mammalian livestock animals.

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Section: Beta Toxin (Cpb)mentioning

confidence: 99%

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Ferreira

Moreira

Cunha

et al. 2016

Toxins

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show abstract

“…It may be possible to create targeted vaccine(s) composed of non‐toxic peptides. That seems feasible, as all C perfringens toxins genes have been cloned and expressed as recombinant proteins (α: Bennett and others 1999, Schoepe and others 2006 ; β: Steinthorsdottir and others 1995 ; α‐β: Bai and others 2006 ; β‐β2: Xu and others 2005 ; ϵ: Goswami and others 1996, Oyston and others 1998 ; ι: Sirard and others 1997 ; enterotoxin: Belyi and Varfolomeeva 2003 ; β2, Gibert and others 1997, Lebrun and others 2007). Another way would be to select strains producing naturally protective immunogenic but non‐toxic variants of the toxins of interest, such as the 1121A/19 strain of C perfringens , which produces a non‐toxic variant of a toxin that induces a protective response against different toxic variants of α (Schoepe and others 2001).…”

Section: Prophylaxismentioning

confidence: 99%

Cattle enterotoxaemia andClostridium perfringens: description, diagnosis and prophylaxis

Lebrun¹,

Mainil

Lindén

2010

Veterinary Record

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“…When these histidines are replaced by other amino acid residues, such as glycine, the hemolytic activity and lethality of the α-toxin are significantly reduced or even eliminated. Nonetheless, its antigenicity can be retained [12][13][14], pointing to a promising strategy for the development of a subunit vaccine against C. perfringens α-toxin [15,16].…”

Section: Introductionmentioning

confidence: 99%

Oral immunization of mice with a probioticLactobacillus caseiconstitutively expressing the α-toxoid induces protective immunity againstClostridium perfringensα-toxin

Gao

Wang

et al. 2019

Virulence

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Clostridium perfringens α-toxin is one of the major virulence factors during C. perfringens infection, causing hemolysis of erythrocytes in various species. Here, genetically engineered Lactobacillus casei (pPG-α/L. casei 393) constitutively expressing the toxoid of C. perfringens α-toxin was generated and its immunogenicity in mice for induction of protective immunity against the αtoxin was evaluated via oral immunization. The α-toxoid was constitutively expressed by pPG-α/ L. casei 393 without a specific inducer, as confirmed by western blotting, laser confocal microscopy, and flow cytometry. In an experiment on BALB/c mice to evaluate the oral immunogenicity of pPG-α/L. casei 393, significant levels of a specific secretory IgA (sIgA) antibody in the intestinal mucus and feces and an IgG antibody in the serum of the probiotic vaccine group were detected after booster immunization (p < 0.05) as compared with the pPG/L. casei 393 and PBS control groups. These antibodies effectively neutralized C. perfringens natural α-toxin. Moreover, significantly higher levels of cytokines IL-2, IL-4, IL-10, IL-12, IL-17, and interferon (IFN) γ in the serum and increased proliferation of spleen lymphocytes obtained from mice orally immunized with pPG-α/L. casei 393 were detected. With a commercial C. perfringens type A inactivated vaccine as a control, immune protection provided by the probiotic vaccine against C. perfringens α-toxin was evaluated, and 90% and 80% protection rates were observed, respectively. Therefore, strain pPGα/L. casei 393 effectively elicited mucosal, humoral, and cellular immunity, suggesting that pPG-α/ L. casei 393 is a promising candidate for development of a vaccine against C. perfringens α-toxin.

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Cloning of α-β fusion gene from Clostridium perfringens and its expression

Cited by 9 publications

References 17 publications

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Cattle enterotoxaemia andClostridium perfringens: description, diagnosis and prophylaxis

Oral immunization of mice with a probioticLactobacillus caseiconstitutively expressing the α-toxoid induces protective immunity againstClostridium perfringensα-toxin

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