Cloning, purification and characterization of novel Cu-containing nitrite reductase from the Bacillus firmus GY-49

Gao, Haofeng; Li, Caiqing; Bandikari, Ramesh; Hu, Nan

doi:10.1007/s11274-017-2383-6

Cited by 16 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…are rarely reported. Gao, et al [40] reported molecular and structural analyses of Cu-NIR in gram-positive bacteria of Bacillus firmus GY-49. The present study provides further evidence for the existence of nirK in Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Sang¹,

Fu²,

Guo³

et al. 2020

Water

View full text Add to dashboard Cite

Overloading of ammonia and nitrite nitrogen in aquaculture can result in toxicity to aquatic animals. In order to eliminate the hazardous substances, a highly efficient denitrifying bacterium, Bacillus sp. SC16, was identified in a fishery pond and isolated subsequently. The strain SC16 could remove nitrate up to 97%, ammonia up to 36.6%, and nitrite up to 99.99% when incubated with nitrate at an initial concentration of 306.9 mg·L−1 for 72 h, ammonia at 165.49 mg·L−1 for 48 h, and nitrite at 200 mg·L−1 for 24 h under aerobic conditions. The nitrite reductase gene was identified as the nirK gene. The transcriptional levels of the nirK gene in strain SC16 incubated with ammonia, nitrate, and nitrite showed similar expression patterns. When the strain SC16 was used to treat the aquaculture water, the concentration of ammonia decreased significantly, from 8.35 mg·L−1 to 4.56 mg·L−1, and there was almost no accumulation of nitrite by the end of experiment. Therefore, the results indicated that Bacillus sp. SC16 could be a promising candidate for aquaculture water treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Sang¹,

Fu²,

Guo³

et al. 2020

Water

View full text Add to dashboard Cite

show abstract

“…In addition, the accumulation of nitrite nitrogen was lower than that in the control treatment in the presence of 0.05 mg Cu 2+ L −1 . A Cu-containing nitrite reductase encoded by the nirK gene has been confirmed to be the key enzyme in the denitrification pathway, and the addition of Cu 2+ could increase the nitrite reductase (NiR) activity, which would result in less nitrite accumulation and improved denitrification efficiency [43,44].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying Arthrobacter nicotianae D51 Strain in the Presence of Copper

et al. 2019

Water

View full text Add to dashboard Cite

A heterotrophic nitrification and aerobic denitrification bacterium, strain D51, was identified as Arthrobacter nicotianae based on morphological, phospholipid fatty acids (PLFAs), and 16S rRNA gene sequence analyses. Further tests demonstrated that strain D51 had the capability to use nitrite, nitrate, or ammonium as the sole nitrogen source in the presence of Cu2+. The maximum removal efficiencies of nitrite, nitrate and ammonium were 68.97%, 78.32%, and 98.70%, respectively. Additionally, the maximum growth rate and denitrification capacity of this strain occurred in the presence of 0.05 mg·L−1 of Cu2+.However, the growth and aerobic denitrification capacity were intensively inhibited by Cu2+ at ≥0.1 mg·L−1. Moreover, gas chromatography indicated that a portion of the nitrogen was transformed into N2O when the nitrite, nitrate, and ammonium were separately used as the sole nitrogen source. This is the first study of the nitrification and denitrification ability of Arthrobacter nicotianae under aerobic conditions, and the first experiment to investigate the impact of Cu2+ concentration on the growth and denitrification ability of this bacteria. The results presented herein extend the known varieties of heterotrophic nitrifying–aerobic denitrifying bacteria and provide useful information regarding the specific bacteria for nitrogen bioremediation of industrial wastewater containing Cu2+.

show abstract

“…14 The reaction was carried out in a 0.5 mL closed vial containing 100 mM potassium phosphate buffer (pH 6.5), 50 mM methyl viologen and 2.5 M sodium nitrite, 1 M sodium chloride and 0.02 mL puried enzyme. 14 The reaction was initiated with the addition of 50 mL sodium dithionite (1 M) and the vial gently swirled. Aer incubation at 37 C for 10 min, the reaction was terminated by vigorous shaking for 3 min.…”

Section: In Vitro Analytical Methods For Detecting the Enzyme Activitymentioning

confidence: 99%

“…The heterogenous expression of recombinant LJ01-NIR in E. coli were puried by Ni 2+ -NTA affinity chromatography (GE, USA) and Superdex 200 gel ltration chromatography (GE, USA) using a fast protein liquid chromatography system (AKTA Purier Explorer, Pharmacia, Uppsala, Sweden), referring to Gao's method with slight modication. 14…”

Section: In Vitro Analytical Methods For Detecting the Enzyme Activitymentioning

confidence: 99%

Isolation, expression, and biochemical characterization: nitrite reductase from Bacillus cereus LJ01

et al. 2020

View full text Add to dashboard Cite

show abstract

Cloning, purification and characterization of novel Cu-containing nitrite reductase from the Bacillus firmus GY-49

Cited by 16 publications

References 44 publications

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Isolation and Characterization of an Aerobic Denitrifier Bacillus sp. SC16 from an Intensive Aquaculture Pond

Characteristics of Heterotrophic Nitrifying and Aerobic Denitrifying Arthrobacter nicotianae D51 Strain in the Presence of Copper

Isolation, expression, and biochemical characterization: nitrite reductase from Bacillus cereus LJ01

Contact Info

Product

Resources

About