Clonostachys viticola sp. nov., a novel species isolated from Vitis vinifera

Abstract

A collection of fungal isolates obtained from crop plants, specifically grapevine and blueberry, in Peru were characterised through morphological and DNA sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS), beta-tubulin (tub2) and translation elongation factor 1-alpha (tef-1α) regions. Isolates produced monomorphic and dimorphic conidiophores typical of members of the genus Clonostachys. Single-and multi-locus gene phylogenies confirmed the isolates as representing members of the genus Clonostachys, more closely related to species in the subgenus Bionectria. In phylogenetic analyses the isolates grouped in two separate clades, one corresponding to the species Clonostachys pseudochroleuca and the other one distinct from all known species of the genus Clonostachys. These isolates are recognized as representing a novel species species for which the name Clonostachys viticola is proposed. Clonostachys chloroleuca CBS 141588 Clonostachys chloroleuca CBS 141592 Clonostachys chloroleuca CBS 141589 Clonostachys rhizophaga CBS 361.77 Clonostachys rosea f. cantenulata CBS 154.27 Clonostachys rosea f. rosea CBS 710.86 Clonostachys wenpingii HMAS 172156 Clonostachys byssicola CBS 364.78 Clonostachys eriocamporesiana MFLUCC 17-2620 Clonostachys pseudochroleuca CBS 187.94 Clonostachys pseudochroleuca CML 1940 Clonostachys pseudochroleuca CML 2406 Clonostachys pseudochroleuca CAA 948 Clonostachys pseudochroleuca MUM 18.50/CAA 947 Clonostachys pseudochroleuca CML 1982 Clonostachys pseudochroleuca CBS 192.94 Clonostachys pseudochroleuca CML 1983 Clonostachys viticola CAA 946 Clonostachys viticola CAA 945 Clonostachys viticola MUM 18.51/CAA 944 Clonostachys rogersoniana CML 1923 Clonostachys rogersoniana CML 1926 Clonostachys buxi CBS 696.93 Clonostachys candelabrum CBS 504.67 Calonectria ilicicola CBS 190.50

Assessing the Biocontrol Potential of Clonostachys Species Isolated as Endophytes from Coffea Species and as Mycoparasites of Hemileia Rusts of Coffee in Africa

“…It revealed that the collection of Clonostachys isolates from coffee and Hemileia uredinia belonged to Clonostachys byssicola , C. rhizophaga and C. rosea . These are well-known taxa that have already been described and discussed in detail in the literature [ 29 , 45 , 46 ].…”

“…Clonostachys species have been isolated mainly from soil and litter, but there are also records from bryophytes, wood, bark, black pepper, grapevines, insects, nematodes and even wine [ 46 , 49 , 50 ]. Some Clonostachys species have also been reported as mycoparasites, including Botrytis cinerea , an important pathogen that causes gray mold on numerous crops [ 51 , 52 , 53 ].…”

Three New Species of Clonostachys (Hypocreales, Ascomycota) from China

“…Clonostachys Corda, typified by C. araucaria Corda, is characterized by solitary to gregarious, subglobose or globose to ovoid perithecia that are white, yellow, pale orange, tan, or brown; perithecial walls are KOH− and LA−; there are narrowly clavate to clavate asci containing eight ascospores; it produces penicillium-, verticillium-, gliocladium-, or acremonium-like conidiophores, cylindrical to narrowly flask-shaped phialides, and ellipsoidal to subfusiform conidia [ 1 ]. Members of the genus usually have a broad range of lifestyles and occur on the bark of recently dead trees, decaying leaves, and less frequently on other fungi, nematodes, and insects [ 1 , 2 , 3 ]. They are economically important in the fields of pharmaceutics and agriculture [ 4 ].…”

“…About 100 names have been created under the genus Clonostachys ( (accessed on 1 July 2022)), among which 65 species are commonly accepted [ 2 , 3 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. Twenty-four species are known from China [ 2 , 7 , 21 ].…”

Phylogeny and systematics of the genus Clonostachys

“…These known species are commonly found in soils, litter, and dead plant substrata as saprotrophs. They have also been reported as endophytes and epiphytes of living plants (Torcato et al, 2020). Another aspect of the biology of Clonostachys species is their unspecific parasitic ability.…”