Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of bisoprolol in human plasma

Giebułtowicz, Joanna; Kojro, Grzegorz; Buś-Kwaśnik, Katarzyna; Rudzki, Piotr J.; Marszałek, Ryszard; Leś, Andrzej; Wroczyński, Piotr

doi:10.1016/j.chroma.2015.10.076

Cited by 24 publications

(10 citation statements)

References 36 publications

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“…Despite that, some parameters in this procedure need to be optimized, including surfactant concentration, pH, extraction time, and temperature. Furthermore, it is believed that the CPE method can be extensively applied because of its simplicity, rapid execution speed, and least environmental impact (Giebultowicz et al, 2015;Giebultowicz, Kojro, Piotrowski, Kulakowski, & Wroczynski, 2016;Kojro, Rudzki, Pisklak, & Giebultowicz, 2019).…”

Section: Conventional Pretreatment Methodsmentioning

confidence: 99%

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Chen

Gao

Zhong

2020

Biomedical Chromatography

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Chromatographic method has long been recognized as the most widely used separation method in bioanalytical research. However, the relatively low sensitivity of existing chromatographic methods remains a significant challenge, as the requirements for experimental procedures become more demanding. This review discusses the main causes for the low sensitivity of chromatographic methods and aims to introduce different technologies for enhancing their sensitivity in the following aspects: (i) different pretreatment methods for improving clean‐up efficiency and recovery; (ii) derivatization step for altering the chromatographic behavior of analytes and enhancing MS ionization efficiency; (iii) optimal LC–MS conditions and appropriate separation mechanism; and (iv) applications of other chromatographic methods, including miniaturized LC, 2D‐LC, 2D‐GC, and supercritical fluid chromatography. Altogether, this review is devoted to summarizing the recent technologies reported in the literature and providing new strategies for the detection of bioanalytes.

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Section: Conventional Pretreatment Methodsmentioning

confidence: 99%

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Chen

Gao

Zhong

2020

Biomedical Chromatography

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“…A possible reason is the problem of ion-suppression by detergents used in this extraction method. However, our previous results showed that the matrix effect (ME) for bisoprolol was comparable when serum was extracted by LLE and CPE [11].…”

Section: Introductionmentioning

confidence: 92%

Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of antazoline in human plasma

Giebułtowicz

Kojro

Piotrowski

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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“…On the other hand, too high concentration of surfactant is associated with a high volume of the micelle-rich phase, and the unwanted dilution of the analyte [5,10,32]. The range of tested Triton X-114 concentrations, added to samples in multi-compound determination, varied from 1.5 to 9% (w/v) [1,2,4,5,8,32], with the most frequently used concentration being 4% [32]. In this study, two variants of Triton concentration (1.5 and 6%) were studied.…”

Section: The Effects Of Triton X-114mentioning

confidence: 99%

Determination of Antidepressants in Human Plasma by Modified Cloud-Point Extraction Coupled with Mass Spectrometry

et al. 2020

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Cloud-point extraction (CPE) is rarely combined with liquid chromatography coupled to mass spectrometry (LC–MS) in drug determination due to the matrix effect (ME). However, we have recently shown that ME is not a limiting factor in CPE. Low extraction efficiency may be improved by salt addition, but none of the salts used in CPE are suitable for LC–MS. It is the first time that the influences of a volatile salt—ammonium acetate (AA)—on the CPE extraction efficiency and ME have been studied. Our modification of CPE included also the use of ethanol instead of acetonitrile to reduce the sample viscosity and make the method more environmentally friendly. We developed and validated CPE–LC–MS for the simultaneous determination of 21 antidepressants in plasma that can be useful for clinical and forensic toxicology. The selected parameters included Triton X-114 concentration (1.5 and 6%, w/v), concentration of AA (0, 10, 20 and 30%, w/v), and pH (3.5, 6.8 and 10.2). The addition of 10% of AA increased recovery twice. For 20 and 30% (w/v) of AA, three phases were formed that prolonged the extraction process. The developed CPE method (6% Triton X-114, 10% AA, pH 10.2) was successfully validated through LC–MS/MS simultaneous determination of 21 antidepressants in human plasma. The linearity was in the range of 10–750 ng/mL (r2 > 0.990).

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Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of bisoprolol in human plasma

Cited by 24 publications

References 36 publications

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Technologies to improve the sensitivity of existing chromatographic methods used for bioanalytical studies

Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of antazoline in human plasma

Determination of Antidepressants in Human Plasma by Modified Cloud-Point Extraction Coupled with Mass Spectrometry

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