ClpP is involved in the stress response and degradation of misfolded proteins in Salmonella enterica serovar Typhimurium

Abstract

Components of the ATP-dependent Clp protease complex are found in a wide range of prokaryotic cells and they are often expressed as part of the cellular stress response. To investigate the physiological role of the proteolytic subunit, ClpP, in Salmonella enterica serovar Typhimurium (S. typhimurium) an in-frame deletion of the clpP gene was constructed. Growth experiments revealed that clpP is important for the ability of S. typhimurium to grow under various stressful conditions, such as low pH, elevated temperature and high salt concentrations. Since the stationary-phase sigma factor, RpoS, is a target of the Clp proteolytic complex, the effect of the clpP deletion in the absence of RpoS was examined ; it was observed that growth of the S. typhimurium clpP mutant is affected in both an RpoS-dependent and an RpoS-independent manner. Analysis of the degradation of abnormal puromycyl-containing polypeptides showed that ClpP participates in the proteolysis of such proteins in S. typhimurium. These findings prompted an investigation of the growth of an Escherichia coli clpP mutant under various stress conditions. The growth of this E. coli mutant was affected by heat, salt and low pH, although not to the same extent as observed for the S. typhimurium clpP mutant. The results of this study indicate that the S. typhimurium clpP mutant is generally more sensitive to environmental stress than the E. coli clpP mutant and it is proposed that this is due to a reduced ability to degrade misfolded proteins generated under these conditions.

Isolation of Zn-responsive genes from two accessions of the hyperaccumulator plant Thlaspi caerulescens

“…However, in E. coli a clp mutant was found to be more sensitive to environmental stresses, possibly due to a reduced ability to degrade misfolded proteins (Thomsen et al 2002). If ClpP1 is related to stress responses, its induction by Zn in LC roots may imply an increased need for protein turnover at very high external Zn concentrations.…”

Self‐compartmentalized bacterial proteases and pathogenesis

“…Like other clpP mutants, S. Typhimurium clpP mutants are more susceptible to several in vitro stresses such as heat, acidic pH and high osmolarity (Thomsen et al, 2002). Because clpP is involved in the stress response of S. Typhimurium, it is also possible that the bacteria encounter one or more forms of stress in the host that results in attenuation.…”

Salmonella Flagellin Is Not a Dominant Protective Antigen in Oral Immunization with Attenuated Live Vaccine Strains

“…The serine protease ClpP is normally associated with ClpX, ClpA, or both, which act as molecular chaperones (2,12). In Salmonella enterica serovar Typhimurium, ClpXP protease is also involved in the stress response and degradation of misfolded proteins (15). It was previously reported that the ClpXP protease-depleted mutant of serovar Typhimurium loses virulence and persistently resides in BALB/c mice for long periods after either intraperitoneal (18) or oral (10) infection without causing an overwhelming systemic infection.…”