CNN3 in glioma

Abstract

Background: Gliomas are the most intrinsic type of primary intracranial tumors. The protein encoded by The calponin 3 (CNN3) has been proven to be a member of the calponin family. Its relationships with cervical cancer, colorectal cancer, gastric cancer, and colon cancer have been emphasized by several studies. Our research aims to explore the prognosis value and immunotherapeutic targetability of CNN3 in glioma patients using bioinformatics approach.Methods: CNN3 expression in glioma was analyzed based on GEO and TCGA datasets. Gene expression profiling with clinical information was employed to investigate the correlation between clinicopathological features of glioma patients and relative CNN3 expression levels. Survival analysis was conducted using Kaplan-Meier analysis and the Cox proportional-hazards regression model. Gene set enrichment analysis was conducted to select the pathways significantly enriched for CNN3 associations. Correlations between inflammatory activities, immune checkpoint molecules and CNN3 were probed by gene set variation analysis, correlograms, and correlation analysis.Results: CNN3 was enriched in gliomas, and high expression of CNN3 correlated with worse clinicopathological features and prognosis. Associations between CNN3 and several immune-related pathways were confirmed using a bioinformatics approach. Correlation analysis revealed that CNN3 was associated with inflammatory and immune activities, tumor microenvironment, and immune checkpoint molecules. Conclusion:Our results indicate that high CNN3 expression levels predict poor prognosis, and CNN3 may be a promising immunotherapy target.Abbreviations: CI = confidence interval, CNN3 = The calponin 3, CTLA-4 = cytotoxic T-lymphocyte-associated antigen 4, FDR q-val = false discovery rate q-value, GSEA = gene set enrichment analysis, GSVA = gene set variation analysis, HR = hazard rate, IDH mutations = isocitrate dehydrogenase mutations, IDO = indoleamine 2,3-dioxygenase, KPS = Karnofsky performance score, LGG = low-grade glioma, MDSCs = myeloid-derived suppressor cells, NES = normalized enrichment score, NK = natural killer cells, NOM pval = normalized P-value, OR = odds ratio, PD-1 = programmed cell death protein 1, TAMs = tumor-associated macrophages, TCGA = the cancer genome atlas, Tregs = regulatory T cells.