One of the alternative sources for clean energy is biogas. Generating energy from agrowaste and effluents can lessen environmental impacts. This study was conducted to determine the production of biogas from six potential substrates, i.e. goat dung (GD), chicken dung (CD), fish waste (FW), rice waste (RW), palm oil mill effluent (POME) and sewage sludge (SS) using industrial inoculum as catalysts. Biophysical characteristics were assessed using laboratory-based analyses, whereas the Bio-Methane Potential (BMP) assay was used to measure anaerobic biogas production in mesophilic condition for 20 consecutive days. Results revealed that rice waste with industrial inoculum produces the highest amount of biogas, followed by goat dung, chicken dung, fish waste, palm oil mill effluent and sewage sludge with total amount of 3508.9 mL, 2141 mL, 1885.7 mL, 1546.0 mL, 743.8 mL and 547.7 mL, respectively. In conclusion, all substrates used in this study can be combined with industrial inoculum would serve producing energy in a small-scale industry.