Co-expression of glucose-6-phosphate dehydrogenase and acyl-CoA binding protein enhances lipid accumulation in the yeast Yarrowia lipolytica

Yuzbasheva, Evgeniya Y.; Mostova, E. B.; Андреева, Н. И.; Yuzbashev, Tigran V.; Laptev, Ivan A.; Sobolevskaya, Irina Nikolaevna; Sineoky, S. P.

doi:10.1016/j.nbt.2017.05.008

Cited by 38 publications

(22 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The regions for integration were 1007 bp and 1074 bp. The ACC1 gene was amplified using primers ACC1 ‐ Aar I‐F and ACC1 ‐ Xma JI‐R, digested with Aar I and Xma JI, and introduced into the Bpi I and Xma JI sites in plasmid php4d‐uno (Yuzbasheva et al, ) to generate php4d‐uno‐ ACC1 .…”

Section: Methodsmentioning

confidence: 99%

“…PCR using primers GUT2 ‐chr‐F and GUT2 ‐chr‐R was used to verify chromosomal integration of the cassette, and a verified transformant was designated as W29 (Δ gpd1 Δ gut2 ). The PEX10 gene knock‐down was performed in the strain W29 (Δ gpd1 Δ gut2 Ura‐) as described previously (Yuzbasheva et al, ). The expression plasmid php4d‐uno‐ ACC1 was digested with Sda I and Nde I, and the released cassette was electroporated into the spontaneous mutant S14G19O.…”

Section: Methodsmentioning

confidence: 99%

“…Yeast strains were transformed by electroporation (Yuzbasheva et al, 2015). Auxotrophic derivatives of constructed recombinant strains were obtained using the Cre-lox66/71 recombination system as described previously (Yuzbasheva et al, 2017). The disruption cassette flGPD1-URA3 was released from plasmid pflGPD1-URA3 using Eco72I and transformed into the W29 (Ura-) strain (Yuzbasheva et al, 2017).…”

Section: Construction Of Plasmids and Recombinant Strainsmentioning

confidence: 99%

See 2 more Smart Citations

A metabolic engineering strategy for producing free fatty acids by the Yarrowia lipolytica yeast based on impairment of glycerol metabolism

Yuzbasheva

Mostova

Андреева

et al. 2017

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

In recent years, bio-based production of free fatty acids from renewable resources has attracted attention for their potential as precursors for the production of biofuels and biochemicals. In this study, the oleaginous yeast Yarrowia lipolytica was engineered to produce free fatty acids by eliminating glycerol metabolism. Free fatty acid production was monitored under lipogenic conditions with glycerol as a limiting factor. Firstly, the strain W29 (Δgpd1), which is deficient in glycerol synthesis, was obtained. However, W29 (Δgpd1) showed decreased biomass accumulation and glucose consumption in lipogenic medium containing a limiting supply of glycerol. Analysis of substrate utilization from a mixture of glucose and glycerol by the parental strain W29 revealed that glycerol was metabolized first and glucose utilization was suppressed. Thus, the Δgpd1Δgut2 double mutant, which is deficient also in glycerol catabolism, was constructed. In this genetic background, growth was repressed by glycerol. Oleate toxicity was observed in the Δgpd1Δgut2Δpex10 triple mutant strain which is deficient additionally in peroxisome biogenesis. Consequently, two consecutive rounds of selection of spontaneous mutants were performed. A mutant released from growth repression by glycerol was able to produce 136.8 mg L of free fatty acids in a test tube, whereas the wild type accumulated only 30.2 mg L . Next, an isolated oleate-resistant strain produced 382.8 mg L of free fatty acids. Finely, acyl-CoA carboxylase gene (ACC1) over-expression resulted to production of 1436.7 mg L of free fatty acids. The addition of dodecane promoted free fatty acid secretion and enhanced the level of free fatty acids up to 2033.8 mg L during test tube cultivation.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Construction Of Plasmids and Recombinant Strainsmentioning

confidence: 99%

See 1 more Smart Citation

A metabolic engineering strategy for producing free fatty acids by the Yarrowia lipolytica yeast based on impairment of glycerol metabolism

Yuzbasheva

Mostova

Андреева

et al. 2017

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is speculated that overexpression of ACBP led to a large amount of acyl-CoA synthesis in the cells, leading to the oxidation of some NADPH, thus alleviating the imbalance of the NADPH/NADP + content produced by ZWF1 overexpression. Therefore, ACBP is an important protein for intracellular lipid synthesis that can regulate the production and degradation of intracellular NADPH [61].…”

Section: Acbp Can Regulate the Synthesis And Degradation Of Nadph In mentioning

confidence: 99%

Advances in Understanding the Acyl-CoA-Binding Protein in Plants, Mammals, Yeast, and Filamentous Fungi

Qiu

Zeng

2020

JoF

View full text Add to dashboard Cite

Acyl-CoA-binding protein (ACBP) is an important protein with a size of about 10 kDa. It has a high binding affinity for C12–C22 acyl-CoA esters and participates in lipid metabolism. ACBP and its family of proteins have been found in all eukaryotes and some prokaryotes. Studies have described the function and structure of ACBP family proteins in mammals (such as humans and mice), plants (such as Oryza sativa, Arabidopsis thaliana, and Hevea brasiliensis) and yeast. However, little information on the structure and function of the proteins in filamentous fungi has been reported. This article concentrates on recent advances in the research of the ACBP family proteins in plants and mammals, especially in yeast, filamentous fungi (such as Monascus ruber and Aspergillus oryzae), and fungal pathogens (Aspergillus flavus, Cryptococcus neoformans). Furthermore, we discuss some problems in the field, summarize the binding characteristics of the ACBP family proteins in filamentous fungi and yeast, and consider the future of ACBP development.

show abstract

“…Multicopy of DGAT genes DGA1 or DGA2 overexpression affects the lipid body size and improves lipid accumulation in Y. lipolytica cells. During the -oxidation process, six peroxisomal acyl-CoA oxidases (AOX1-6) encoded by Pox1-6 genes are identified as chainlength-specific for fatty acid degradation [106][107][108][109]. Previous studies demonstrated that the Y. lipolytica strains with Pox1-6 gene mutations impaired fatty acid degrading ability resulting in the increase of high-lipid content in biomass [86,[110][111][112][113].…”

Section: Hydrophobic Substratesmentioning

confidence: 99%

Cellular and metabolic engineering of oleaginous yeastYarrowia lipolyticafor bioconversion of hydrophobic substrates into high‐value products

Soong

Liu

Yoon

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

The non‐conventional oleaginous yeast Yarrowia lipolytica is able to utilize both hydrophilic and hydrophobic carbon sources as substrates and convert them into value‐added bioproducts such as organic acids, extracellular proteins, wax esters, long‐chain diacids, fatty acid ethyl esters, carotenoids and omega‐3 fatty acids. Metabolic pathway analysis and previous research results show that hydrophobic substrates are potentially more preferred by Y. lipolytica than hydrophilic substrates to make high‐value products at higher productivity, titer, rate, and yield. Hence, Y. lipolytica is becoming an efficient and promising biomanufacturing platform due to its capabilities in biosynthesis of extracellular lipases and directly converting the extracellular triacylglycerol oils and fats into high‐value products. It is believed that the cell size and morphology of the Y. lipolytica is related to the cell growth, nutrient uptake, and product formation. Dimorphic Y. lipolytica demonstrates the yeast‐to‐hypha transition in response to the extracellular environments and genetic background. Yeast‐to‐hyphal transition regulating genes, such as YlBEM1, YlMHY1 and YlZNC1 and so forth, have been identified to involve as major transcriptional factors that control morphology transition in Y. lipolytica. The connection of the cell polarization including cell cycle and the dimorphic transition with the cell size and morphology in Y. lipolytica adapting to new growth are reviewed and discussed. This review also summarizes the general and advanced genetic tools that are used to build a Y. lipolytica biomanufacturing platform.

show abstract

Co-expression of glucose-6-phosphate dehydrogenase and acyl-CoA binding protein enhances lipid accumulation in the yeast Yarrowia lipolytica

Cited by 38 publications

References 27 publications

A metabolic engineering strategy for producing free fatty acids by the Yarrowia lipolytica yeast based on impairment of glycerol metabolism

A metabolic engineering strategy for producing free fatty acids by the Yarrowia lipolytica yeast based on impairment of glycerol metabolism

Advances in Understanding the Acyl-CoA-Binding Protein in Plants, Mammals, Yeast, and Filamentous Fungi

Cellular and metabolic engineering of oleaginous yeastYarrowia lipolyticafor bioconversion of hydrophobic substrates into high‐value products

Contact Info

Product

Resources

About