Coagulase gene polymorphism of Staphylococcus aureus isolated from clinical and sub-clinical bovine mastitis in Isfahan and Chaharmahal va Bakhtiari provinces of Iran

Abstract

Mastitis is a common disease in dairy cattle and is an inflammatory response of the breast tissue to bacterial attack to this tissue. Mastitis causes considerable loss to the dairy industry, among the several bacterial pathogens that can cause mastitis; Staphylococcus aureus is probably the most lethal agent because it causes chronic and deep infection in the mammary glands that is extremely difficult to cure. Several virulence factors including coagulase gene are produced by S. aureus and may contribute to its pathogenicity. This study was conducted to investigate the coagulase gene polymorphism of S. aureus isolated from clinical and sub-clinical bovine mastitis milk samples in Isfahan and Chaharmahal va Bakhtiari provinces of Iran. Amplification of the coagulase gene from 86 S. aureus strains isolates by specific primers showed 31 specimens contained 970 bp fragment, and 11 strains contained 730 bp fragment relevant to coa gene (coagulase) in PCR. After enzymatic digestion with AluI, 31 specimens contained three bands: 320, 490, and 160 bp (genotype I) and 11 specimens contained two bands: 490 and 240 bp (genotype VIII) in the RFLP.

Molecular typing of staphylococcus aureus strains from Iranian raw milk and dairy products by coagulase gene polymorphisms

“…The genotype I was the predominant genotype. These findings are in accordance with a previous study by Momtaz et al in this region [19]. In another report by Saei et al [20] in Tabriz and Urmia regions of Iran, similar results have been observed.…”

Typing of Staphylococcus aureus obtained from mastitic milk of cattle and buffalo on the basis of two virulence-associated genes (spa and clfA)

“…[ 6 ] recorded amplicon with a size of approximately 980 bp for all 34 S. aureus . Likewise, other workers [ 26 - 28 ] also found amplicons of same sizes with no polymorphism. Akineden et al .…”

PCR for detection of virulence and antibiotic resistance genes of coagulase positive staphylococcus aureus from clinical mastitis in Egypt

“…The high prevalence in the present study may be correlated to the transmission of infection by milker's hands, contaminated equipments and bad hygienic environment in small holder cows [25]. Several virulence factors were produced by S. aureus, including Coagulase protein, which is important in the pathogenicity [26] through turn fibrinogen to fibrin, then lead to abscess formation and persistence of microorganism in host tissue [27].Coagulase protein is considered to be a virulence factor in intrammmary infection and can be used as a simple and effective method for typing of S. aureus isolates from bovine mastitis. [28].…”

“…This finding agree with [30] achieved that S. aureus isolates give 5 different PCR products of Coa gene. Furthermore, [26] stated that coagulase gene had different PCR products indicating the polymorphism of the gene. Moreover [28]showed amplification of the coa gene of S. aureus isolates from mastitic cow produces 27 different PCR-products, which ranged from 579 to 1442 bp.…”