Coaxial additive manufacture of biomaterial composite scaffolds for tissue engineering

Cornock, Rhys; Beirne, Stephen; Thompson, Brianna C.; Wallace, Gordon G.

doi:10.1088/1758-5082/6/2/025002

Cited by 38 publications

(35 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The shell has sufficient strength to serve as a scaffold and the core is soft which is beneficial for cell survival 28 . This superior biological structure lays the foundation for its applications, such in as tissue engineering and as drug release platforms 29 . Also, the thick and porous cell-laden structures are sufficient for supporting initial cell viability, as well as supporting functions, such as vascularization, differentiations and tissue heterogeneity 30–32 .…”

Section: Discussionmentioning

confidence: 99%

Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Dai

Liu

Ouyang

et al. 2017

Sci Rep

118

View full text Add to dashboard Cite

Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, some cell-cell interactions are limited by the existing hydrogel. To better mimic tumor microenvironment, self-assembled multicellular heterogeneous brain tumor fibers have been fabricated by a custom-made coaxial extrusion 3D bioprinting system, with high viability, proliferative activity and efficient tumor-stromal interactions. Therein, in order to further verify the sufficient interactions between tumor cells and stroma MSCs, CRE-LOXP switch gene system which contained GSCs transfected with “LOXP-STOP-LOXP-RFP” genes and MSCs transfected with “CRE recombinase” gene was used. Results showed that tumor-stroma cells interacted with each other and fused, the transcription of RFP was higher than that of 2D culture model and control group with cells mixed directly into alginate, respectively. RFP expression was observed only in the cell fibers but not in the control group under confocal microscope. In conclusion, coaxial 3D bioprinted multicellular self-assembled heterogeneous tumor tissue-like fibers provided preferable 3D models for studying tumor microenvironment in vitro, especially for tumor-stromal interactions.

show abstract

Section: Discussionmentioning

confidence: 99%

Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Dai

Liu

Ouyang

et al. 2017

Sci Rep

118

View full text Add to dashboard Cite

show abstract

“…From this basic set-up there are many interesting variations, for example coaxial needle design [37] or complex robotic joints to increase the degree of geometric freedom [38]. In biofabrication the resolution of Brought to you by | Freie Universität Berlin Authenticated Download Date | 7/6/15 4:02 PM this technique is mainly limited by requirement 1 mentioned in the chapter above.…”

Section: Extrusion Printingmentioning

confidence: 99%

Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

DeSimone

Schacht

Jüngst

et al. 2015

Pure and Applied Chemistry

View full text Add to dashboard Cite

Despite significant investment in tissue engineering over the past 20 years, few tissue engineered products have made it to market. One of the reasons is the poor control over the 3D arrangement of the scaffold's components. Biofabrication is a new field of research that exploits 3D printing technologies with high spatial resolution for the simultaneous processing of cells and biomaterials into 3D constructs suitable for tissue engineering. Cell-encapsulating biomaterials used in 3D bioprinting are referred to as bioinks. This review consists of: (1) an introduction of biofabrication, (2) an introduction of 3D bioprinting, (3) the requirements of bioinks, (4) existing bioinks, and (5) a specific example of a recombinant spider silk bioink. The recombinant spider silk bioink will be used as an example because its unmodified hydrogel format fits the basic requirements of bioinks: to be printable and at the same time cytocompatible. The bioink exhibited both cytocompatible (selfassembly, high cell viability) and printable (injectable, shear-thinning, high shape fidelity) qualities. Although improvements can be made, it is clear from this system that, with the appropriate bioink, many of the existing faults in tissue-like structures produced by 3D bioprinting can be minimized.

show abstract

“…During the past few years bioprinting has emerged as a class of enabling technologies towards the solution of such a challenge, due to their unparalleled versatility of depositing complex tissue patterns at high fidelity and reproducibility in an automated or semi-automated manner 31,32,33 . Sacrificial bioprinting 34,35,36,37,38 , embedded bioprinting 39,40,41 , and hollow structure bioprinting/biofabrication 42,43,44,45,46,47,48,49,50,51,52,53 have all demonstrated the feasibility of generating vascular or vascularized tissues.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Zhang¹,

Pi²,

Genderen³

2017

JoVE

View full text Add to dashboard Cite

Engineering vascularized tissue constructs and organoids has been historically challenging. Here we describe a novel method based on microfluidic bioprinting to generate a scaffold with multilayer interlacing hydrogel microfibers. To achieve smooth bioprinting, a core-sheath microfluidic printhead containing a composite bioink formulation extruded from the core flow and the crosslinking solution carried by the sheath flow, was designed and fitted onto the bioprinter. By blending gelatin methacryloyl (GelMA) with alginate, a polysaccharide that undergoes instantaneous ionic crosslinking in the presence of select divalent ions, followed by a secondary photocrosslinking of the GelMA component to achieve permanent stabilization, a microfibrous scaffold could be obtained using this bioprinting strategy. Importantly, the endothelial cells encapsulated inside the bioprinted microfibers can form the lumen-like structures resembling the vasculature over the course of culture for 16 days. The endothelialized microfibrous scaffold may be further used as a vascular bed to construct a vascularized tissue through subsequent seeding of the secondary cell type into the interstitial space of the microfibers. Microfluidic bioprinting provides a generalized strategy in convenient engineering of vascularized tissues at high fidelity.

show abstract

Coaxial additive manufacture of biomaterial composite scaffolds for tissue engineering

Cited by 38 publications

References 35 publications

Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Biofabrication of 3D constructs: fabrication technologies and spider silk proteins as bioinks

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Contact Info

Product

Resources

About