Cochlear implantation for chronic electrical stimulation in the mouse

Irving, Samantha; Trotter, M I; Fallon, James B; Millard, Rodney; Shepherd, Robert K.; Wise, Andrew K.

doi:10.1016/j.heares.2013.09.005

Cited by 24 publications

(13 citation statements)

References 36 publications

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“…SGNs were identified within Rosenthal’s canal and counted. Only SGNs exhibiting a clear nucleus were counted using previously published techniques [32, 33, 51, 55, 56]. Data was collected from five non-continuous sections with a separation of 72 μm ensuring that no cell was counted more than once.…”

Section: Methodsmentioning

confidence: 99%

Improved Auditory Nerve Survival with Nanoengineered Supraparticles for Neurotrophin Delivery into the Deafened Cochlea

et al. 2016

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Cochlear implants electrically stimulate spiral ganglion neurons (SGNs) in order to provide speech cues to severe-profoundly deaf patients. In normal hearing cochleae the SGNs depend on endogenous neurotrophins secreted by sensory cells in the organ of Corti for survival. SGNs gradually degenerate following deafness and consequently there is considerable interest in developing clinically relevant strategies to provide exogenous neurotrophins to preserve SGN survival. The present study investigated the safety and efficacy of a drug delivery system for the cochlea using nanoengineered silica supraparticles. In the present study we delivered Brain-derived neurotrophic factor (BDNF) over a period of four weeks and evaluated SGN survival as a measure of efficacy. Supraparticles were bilaterally implanted into the basal turn of cochleae in profoundly deafened guinea pigs. One ear received BDNF-loaded supraparticles and the other ear control (unloaded) supraparticles. After one month of treatment the cochleae were examined histologically. There was significantly greater survival of SGNs in cochleae that received BDNF supraparticles compared to the contralateral control cochleae (repeated measures ANOVA, p = 0.009). SGN survival was observed over a wide extent of the cochlea. The supraparticles were well tolerated within the cochlea with a tissue response that was localised to the site of implantation in the cochlear base. Although mild, the tissue response was significantly greater in cochleae treated with BDNF supraparticles compared to the controls (repeated measures ANOVA, p = 0.003). These data support the clinical potential of this technology particularly as the supraparticles can be loaded with a variety of therapeutic drugs.

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Section: Methodsmentioning

confidence: 99%

Improved Auditory Nerve Survival with Nanoengineered Supraparticles for Neurotrophin Delivery into the Deafened Cochlea

et al. 2016

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“…Connect the head-stage and pre-amplifier to the auditory processor via the optic fiber port. 5. Check the impedance of the active and reference electrode.…”

Section: Pre-deafening Acoustic Auditory Brainstem Response (Aabr)mentioning

confidence: 99%

“…Previous studies have shown the feasibility of CI implantation in mice 5,6,7 . Irving et al demonstrated that chronic intracochlear electrical stimulation can be achieved for up to one month.…”

Section: Introductionmentioning

confidence: 99%

“…Acute stimulation was also performed but the recordings were not presented. They showed that cauterizing the stapedial artery had no significant effect on the hearing threshold or the number of spiral ganglion neurons and that topical application of the aminoglycoside neomycin, an ototoxic drug, was an effective deafening procedure in mice 5 . Soken et al described a modified dorsal approach to the mouse cochlea through the round window to better preserve hearing status 6 .…”

Section: Introductionmentioning

confidence: 99%

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Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice

Navntoft

Marozeau

Barkat³

2019

JoVE

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Cochlear implants (CIs) are neuroprosthetic devices that can provide a sense of hearing to deaf people. However, a CI cannot restore all aspects of hearing. Improvement of the implant technology is needed if CI users are to perceive music and perform in more natural environments, such as hearing out a voice with competing talkers, reflections, and other sounds. Such improvement requires experimental animals to better understand the mechanisms of electric stimulation in the cochlea and its responses in the whole auditory system. The mouse is an increasingly attractive model due to the many genetic models available. However, the limited use of this species as a CI model is mainly due to the difficulty of implanting small electrode arrays. More details about the surgical procedure are therefore of great interest to expand the use of mice in CI research. In this report, we describe in detail the protocol for acute deafening and cochlear implantation of an electrode array in the C57BL/6 mouse strain. We demonstrate the functional efficacy of this procedure with electrically-evoked auditory brainstem response (eABR) and show examples of facial nerve stimulation. Finally, we also discuss the importance of including a deafening procedure when using a normally hearing animal. This mouse model provides a powerful opportunity to study genetic and neurobiological mechanisms that would be of relevance for CI users.

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“…the models by Frijns et al (1995); Malherbe et al 2013and Wong et al (2016)). Cat is also a popular experimental animal model (Hatsushika et al 1990) for which a 3D model has recently been developed (Kang et al 2015), while other experimental animal cochlear models like mouse (Irving et al 2013),…”

Section: Animal Modelsmentioning

confidence: 99%

Three-dimensional models of cochlear implants: A review of their development and how they could support management and maintenance of cochlear implant performance

Hanekom

2016

Network: Computation in Neural Systems

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Three-dimensional (3D) computational modeling of the auditory periphery forms an integral part of modern-day research in cochlear implants (CIs). These models consist of a volume conduction description of implanted stimulation electrodes and the current distribution around these, coupled with auditory nerve fiber models. Cochlear neural activation patterns can then be predicted for a given input stimulus. The objective of this article is to present the context of 3D modeling within the field of CIs, the different models, and approaches to models that have been developed over the years, as well as the applications and potential applications of these models. The process of development of 3D models is discussed, and the article places specific emphasis on the complementary roles of generic models and user-specific models, as the latter is important for translation of these models into clinical application.

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Cochlear implantation for chronic electrical stimulation in the mouse

Cited by 24 publications

References 36 publications

Improved Auditory Nerve Survival with Nanoengineered Supraparticles for Neurotrophin Delivery into the Deafened Cochlea

Improved Auditory Nerve Survival with Nanoengineered Supraparticles for Neurotrophin Delivery into the Deafened Cochlea

Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice

Three-dimensional models of cochlear implants: A review of their development and how they could support management and maintenance of cochlear implant performance

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