2022
DOI: 10.3389/fmicb.2022.967567
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CodY is modulated by YycF and affects biofilm formation in Staphylococcus aureus

Abstract: BackgroundStaphylococcus aureus (S. aureus) is the leading cause of various infective diseases, including topical soft tissue infections. The goals of this study were to investigate the roles of YycF and CodY in the regulation of biofilm formation and pathogenicity.MethodsElectrophoretic mobility shift assay (EMSA) was conducted to validate the bound promoter regions of YycF protein. We constructed the codY up-regulated or down-regulated S. aureus mutants. The biofilm biomass was determined by crystal violet m… Show more

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Cited by 3 publications
(3 citation statements)
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“…Therefore, without the inhibition of YycF on CodY, the repression effect of CodY will be augmented and the biofilm and other associated pathways will be significantly inhibited as another antibiofilm mechanism of our composite hydrogel. 82 With its sharp edge as a nanoknife, GO can mechanically shear the biofilm and cellular membrane, resulting in a physical antibacterial effect and enhanced transferring effectiveness. In addition, GO has a strong impact on the proliferation and differentiation of the implemented stem cell.…”
Section: ■ Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Therefore, without the inhibition of YycF on CodY, the repression effect of CodY will be augmented and the biofilm and other associated pathways will be significantly inhibited as another antibiofilm mechanism of our composite hydrogel. 82 With its sharp edge as a nanoknife, GO can mechanically shear the biofilm and cellular membrane, resulting in a physical antibacterial effect and enhanced transferring effectiveness. In addition, GO has a strong impact on the proliferation and differentiation of the implemented stem cell.…”
Section: ■ Discussionmentioning
confidence: 99%
“…CodY as a global repressor controls many metabolisms in bacteria including biofilm formation. Therefore, without the inhibition of YycF on CodY, the repression effect of CodY will be augmented and the biofilm and other associated pathways will be significantly inhibited as another antibiofilm mechanism of our composite hydrogel …”
Section: Discussionmentioning
confidence: 99%
“…For SEM analysis, the S. aureus strain of interest was cultured in 6-well cell culture according to previous studies [ 30 , 31 ]. After 24 h of incubation, the biofilms were washed twice with PBS to remove nonadherent bacteria and fixed with 2.5% glutaraldehyde at 4°C for 4 h. Then, the biofilm samples were dehydrated and dried in a critical point dryer.…”
Section: Methodsmentioning
confidence: 99%